Correlation of mTOR and MRP1 Expression in Patients with Diffuse Large B-cell Lymphoma

JIA Cundong; LI Xiaobo; LI Yanhong; LIANG Liping; BAI Jingping

doi:10.3971/j.issn.1000-8578.2015.09.009

Cancer Research on Prevention and Treatment > 2015 > 42(09): 900-904. > DOI: 10.3971/j.issn.1000-8578.2015.09.009

JIA Cundong, LI Xiaobo, LI Yanhong, LIANG Liping, BAI Jingping. Correlation of mTOR and MRP1 Expression in Patients with Diffuse Large B-cell Lymphoma[J]. Cancer Research on Prevention and Treatment, 2015, 42(09): 900-904. DOI: 10.3971/j.issn.1000-8578.2015.09.009

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Correlation of mTOR and MRP1 Expression in Patients with Diffuse Large B-cell Lymphoma

1.Department of Medical Oncology, Affiliated Tumor Hospital, Xinjiang Medical University,Urumqi 830011, China; 2. Xinjiang Technical Institute of Physics&Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; 3. Department of Pathology, Affiliated Tumor Hospital,Xinjiang Medical University, Urumqi 830011, China; 4. Department of Bone Oncology, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China

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Received Date: September 18, 2014
Revised Date: January 22, 2015

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Abstract

Objective To investigate the expression and significance of the mammalian target of rapamycin(mTOR) and multidrug resistance protein 1(MRP1)gene in patients with diffuse large B-cell lymphoma(DLBCL). Methods A total of 109 patients with DLBCL diagnosed from Jan., 2010 to Jun., 2013 were collected from the Affiliated Tumor Hospital, Xinjiang Medical University. The expression of phosphorylated mTOR(p-mTOR) protein and MRP1 mRNA were examined by Western blot and RT-PCR, respectively. Kaplan-Meier method was used for survival analysis. Results The expression of p-mTOR and MRP1 mRNA were 58.7% and 56.9%, respectively(P=0.003). The expression of p-mTOR and MRP1 mRNA in non-germinal center B-cell like(non-GCB) subgroups were significantly higher than those in GCB subgroups(P<0.05). At a median follow-up of 18 months (range 2-52 months), the 3-year progression-free survival (PFS) of patients with positive and negative p-mTOR expression were 23% and 69%(P=0.003). The 3-year PFS of patients with positive and negative MRP1 expression were 39% and 62%(P=0.042). Conclusion Activation of mTOR signaling pathway and MRP1 overexpression play important roles in the development of DLBCL, moreover, MRP1 expression is closely related to mTOR expression.
- Diffuse large B-cell lymphoma,
- Mammalian target of rapamycin(mTOR),
- Multidrug resistance protein 1(MRP1)

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