Inhibitive Effect of Artemether on Brain Glioma Angiogenesis in SD Rat

To investigate inhibitory effect of Artmeter （Artm）on brain glioma angiogenesis in SD rat. Methods MTT (Methy thiazolyl tetrazolium) assay was used to evaluate the inhibitory effect of Artemether treatment on C6 glioma cells. Forty (20 male and 20 female) SD rats which was subcutaneous implantation with SD rat C6 glioma cell (1×10⁶/μl) in cerebral cortex were divided into 5 groups randomly, each group had 8 rats.All were taken orally.After 3 day of implantation the treatment was started and continued until 10^th day, and the rat killed 20^th day. Whole brain was fixed and length-path (a mm) and short- path (b mm) of tumors were measured. Volume of tumor was calculated following formula: V (mm3) = a²bπ∕6. Micro-vascular dense (MVD) was observed under the microscopy by immunohistochemistry. Results The value of Micro-vascular dense in different therapy groups was groupⅠ（39±4）,group Ⅱ（29±6），group Ⅲ （12±8）and group Ⅳ（10±5）, normal saline control group is （52±7）,respectively,Micro-vascular dense (MVD) in different therapy groups was significant lower than that in control group (P<0.05, P<0.01 respectively) and volume of brain glioma in different therapy groups is significant smaller than that in control group (P<0.05, P<0.01 respectively). Conclusion There is significant inhibitory effect of Artmeter on brain glioma angiogenesis in SD rat in some arrange dosage. The mechanism that Artemether inhibits brain glioma growth and metastasis may be penetrating the blood-brain barrier by inhibiting angiogenesis.