Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu

HAN Zheng; HUANG Xiaodong; LIU Meng; ZHU Qingxi; TAN Jie; LIU Weijie; CHEN Wei; ZOU Yanli; CAI Yishan; HUANG Shasha; TIAN Xia

doi:10.3971/j.issn.1000-8578.2019.18.1927

Cancer Research on Prevention and Treatment > 2019 > 46(8): 696-701. > DOI: 10.3971/j.issn.1000-8578.2019.18.1927

HAN Zheng, HUANG Xiaodong, LIU Meng, ZHU Qingxi, TAN Jie, LIU Weijie, CHEN Wei, ZOU Yanli, CAI Yishan, HUANG Shasha, TIAN Xia. Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu[J]. Cancer Research on Prevention and Treatment, 2019, 46(8): 696-701. DOI: 10.3971/j.issn.1000-8578.2019.18.1927

Citation:

PDF (9786 KB)

Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu

Department of Gastroenterology, The Third Hospital of Wuhan, Wuhan 430060, China Corresponding Author: TIAN Xia, E-mail: hcwy100@163. com

More Information

Corresponding author:
TIAN Xia, E-mail: hcwy100@163.com
Received Date: December 10, 2018
Revised Date: April 02, 2019
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of Noscapine(Nos) on drug resistance of 5-Fu-resistant human colon cancer cell lines and its mechanism.
Methods
Drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu were established. MTT assay was used to test IC₅₀ and cell morphology was observed. Cell cycle and apoptosis were determined by flow cytometry. RT-qPCR and Western blot were used to detect the expression and phosphorylation of P38 and the expression of drug resistance-related proteins.
Results
The drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu were successfully constructed. Compared with control group, cell cycle of colon cancer drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu after Nos intervention was significantly blocked at G₀/G₁ phase (P < 0.01), the apoptosis rate was increased, the expression of P38 and phosphorylation were significantly inhibited (P < 0.01), and the expression of MRP, LRP and P-glycoprotein were significantly decreased (P < 0.01).
Conclusion
Noscapine has a certain toxic effect on 5-Fu-resistant human colon cancer cells and reduces drug resistance. The mechanism may be related to the inhibition of P38 expression and phosphorylation.
- Noscapine,
- Colon cancer,
- Drug resistant cell,
- P38,
- Resistance-related protein

FullText(HTML)

References (15)

References

[1]	Miller KD, Siegel RL, Lin CC, et al. Cancer treatment and survivorship statistics, 2016[J]. CA Cancer J Clin, 2016, 66(4): 271-289. doi: 10.3322/caac.21349
[2]	Zhu J, Tan Z, Hollis-Hansen K, et al. Epidemiological Trends in Colorectal Cancer in China: An Ecological Study[J]. Dig Dis Sci, 2017, 62(1): 235-243. doi: 10.1007/s10620-016-4362-4
[3]	Gao Y, Xiao X, Zhang C, et al. Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways[J]. J Pineal Res, 2016, 62(2). http://cn.bing.com/academic/profile?id=322cf8bccb826961b0ce4938bb264b4e&encoded=0&v=paper_preview&mkt=zh-cn
[4]	韩峥, 黄晓东, 刘蒙, 等.人结肠癌那可丁耐药细胞株HT-29/Nos、LoVo/Nos、SW480/Nos的构建及其生物特性探究[J].广东医学, 2018, 39(2): 176-180. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gdyx201802004 Han Z, Huang XD, Liu M, et al. The establishment and biological characteristics of noscapine resistant human colon carcinoma cell line HT-29/Nos, LoVo/Nos and SW480/Nos[J]. Guangdong Yi Xue, 2018, 39(2): 176-180. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gdyx201802004
[5]	Li Y, Smolke CD. Engineering biosynthesis of the anticancer alkaloid noscapine in yeast[J]. Nat Commun, 2016, 7: 12137. doi: 10.1038/ncomms12137
[6]	Tomar V, Kukreti S, Prakash S, et al. Noscapine and its Analogs as Chemotherapeutic Agent: Current updates[J]. Curr Top Med Chem, 2017, 17(2): 174-188. http://cn.bing.com/academic/profile?id=6a2aa6b5a392f335ee3030ade16fbb05&encoded=0&v=paper_preview&mkt=zh-cn
[7]	Zhang C, Ma Q, Shi Y, et al. A novel 5-fluorouracil-resistant human esophageal squamous cell carcinoma cell line Eca-109/5-FU with significant drug resistance-related characteristics[J]. Oncol Rep, 2017, 37(5): 2942-2954. doi: 10.3892/or.2017.5539
[8]	Chen NN, Wei F, Wang L, et al. Tumor Necrosis Factor Alpha Induces Neural Stem Cell Apoptosis Through Activating p38 MAPK Pathway[J]. Neurochem Res, 2016, 41(11): 3052-3062. doi: 10.1007/s11064-016-2024-8
[9]	Turjanski AG, Hummer G, Gutkind JS. How mitogen-activated protein kinases recognize and phosphorylate their targets: A QM/MM study[J]. J Am Chem Soc, 2009, 131(17): 6141-6148. doi: 10.1021/ja8071995
[10]	Cordero-Herrera I, Martín MA, Bravo L, et al. Epicatechin gallate induces cell death via p53 activation and stimulation of p38 and JNK in human colon cancer SW480 cells[J]. Nutr Cancer, 2013, 65(5): 718-728. doi: 10.1080/01635581.2013.795981
[11]	Koul HK, Pal M, Koul S. Role of p38 MAP Kinase Signal Transduction in Solid Tumors[J]. Genes Cancer, 2013, 4(9-10): 342-359. doi: 10.1177/1947601913507951
[12]	Shi X, Wu S, Yang Y, et al. AQP5 silencing suppresses p38 MAPK signaling and improves drug resistance in colon cancer cells[J]. Tumor Biol, 2014, 35(7): 7035-7045. doi: 10.1007/s13277-014-1956-3
[13]	Yang AK, Zhou ZW, Wei MQ, et al. Modulators of multidrug resistance associated proteins in the management of anticancer and antimicrobial drug resistance and the treatment of inflammatory diseases[J]. Curr Top Med Chem, 2010, 10(17): 1732-1756. doi: 10.2174/156802610792928040
[14]	Cai B, Miao Y, Liu Y, et al. Nuclear multidrug-resistance related protein 1 contributes to multidrug-resistance of mucoepidermoid carcinoma mainly via regulating multidrug-resistance protein 1: a human mucoepidermoid carcinoma cells model and spearman's rank correlation analysis[J]. PLoS One, 2013, 8(8): e69611. doi: 10.1371/journal.pone.0069611
[15]	孔易, 胡占峰, 徐树雷, 等.多药耐药相关蛋白和肺耐药蛋白在大肠癌中的表达及其意义[J].广东医学, 2014, 35(2): 255-257. http://d.old.wanfangdata.com.cn/Periodical/gdyx201402031 Kong Y, Hu ZF, Xu SL, et al. The significance of the expression of multidrug resistance related proteins and lung resistance proteins in colorectal cancer[J]. Guangdong Yi Xue, 2014, 35(2): 255-257. http://d.old.wanfangdata.com.cn/Periodical/gdyx201402031

[1]	DAI Nan, ZHAO Xiaolong, DAI Xiaoyan, LI Mengxia. Effect of Exosomal APE1 on Sensitivity of NSCLC A549 Cells to Cisplatin[J]. Cancer Research on Prevention and Treatment, 2020, 47(7): 492-497. DOI: 10.3971/j.issn.1000-8578.2020.19.1609
[2]	HUANG Zejian, FANG Chang, YU Baodan, CHENG Qing, LYU Ping. 3E10 Targeting CD24 Enhances Chemotherapy Sensitivity of Hepatocellular Carcinoma HuH-7 Cells[J]. Cancer Research on Prevention and Treatment, 2018, 45(8): 540-544. DOI: 10.3971/j.issn.1000-8578.2018.17.1484
[3]	CAI Rui, CHEN Qiuqiu, JIANG Wei. 5-azacytidine Increases Radiation Sensitivity of Nasopharyngeal Carcinoma Cell Line C666-1[J]. Cancer Research on Prevention and Treatment, 2017, 44(2): 94-97. DOI: 10.3971/j.issn.1000-8578.2017.02.003
[4]	ZHANG Juping, SHI Yehui, JIA Yongsheng, ZHOU Liyan, TONG Zhongsheng. GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP[J]. Cancer Research on Prevention and Treatment, 2016, 43(6): 459-462. DOI: 10.3971/j.issn.1000-8578.2016.06.005
[5]	LI Wei, PENG Junqin, LI Jiansheng, TANG Rijie. MR Apparent Diffusion Coefficient Predicts Sensitivity of Nasopharyngeal Carcinoma to Radiotherapy and Related Factors[J]. Cancer Research on Prevention and Treatment, 2015, 42(12): 1221-1226. DOI: 10.3971/j.issn.1000-8578.2015.12.011
[6]	HU Lili, YIN Yanjun, ZHONG Wenjuan, QIU Feng. miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003
[7]	CAO Xinmei, ZHANG Daiquan, XIA Jiyi, WANG Xu, GAO Yan, XIONG Wei. Effects of HER2 shRNA on Chemotherapy Sensitivity of Mouse Lewis Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 366-368. DOI: 10.3971/j.issn.1000-8578.2014.05.004
[8]	Yang Qingshan, Liu Yuanyuan, Jiang Lipeng. Effect of Expression Vector of Human BAG-1 Gene on Radio-sensitivity of Lung Adenocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2012, 39(02): 127-129. DOI: 10.3971/j.issn.1000-8578.2012.02.002
[9]	FANG Chuan, TAN Yan-li, WANG Jia-liang, SHI Yan-fang, SHAN Xiao-song, LI Wei. Primary Culture and Drug Sensitivity of Human Glioma Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(12): 1380-1382. DOI: 10.3971/j.issn.1000-8578.2010.12.012
[10]	ZHANG Wei, GU Min. Geldanamycin Sensitizes Human Breast Cancer Cells to Adriamycin both in vitro and in vivo[J]. Cancer Research on Prevention and Treatment, 2010, 37(10): 1109-1112. DOI: 10.3971/j.issn.1000-8578.2010.10.004

Cited By

Cited by

Periodical cited type(9)

1.	成俊，徐雪峰，李伟. D-CBCT在肺癌容积旋转调强计划精准治疗中的临床应用. 中国CT和MRI杂志. 2024(04): 35-37 .
2.	蒋浩，曹新超，吴桐，郭志斌. 低剂量CT联合外周血循环肿瘤细胞在肺癌早期诊断中的价值研究. 中国CT和MRI杂志. 2024(06): 57-60 .
3.	王晶，翟成凯. 肿瘤自身抗体及CT人工智能在NSCLC早期诊断中的应用研究进展. 解放军医学杂志. 2024(07): 848-854 .
4.	李雪娇. 多排螺旋CT低剂量胸部扫描在肺癌早期筛查中的应用价值分析. 医药前沿. 2024(15): 79-81 .
5.	易梅玲，易芳玲. 双层探测器光谱CT智能剂量调控技术在低剂量胸部体检中的应用价值. 中国医疗器械信息. 2024(22): 8-10+81 .
6.	杏子，彭源. 低剂量CT在肺癌筛查中的价值. 中国防痨杂志. 2024(S2): 234-236 .
7.	陈蔚，蒋伟. 低剂量螺旋CT扫描用于肺癌筛查及鉴别的效果评估. 深圳中西医结合杂志. 2023(01): 71-73 .
8.	慕珂珂，肖凌云，董炎红. 早期肺癌患者血清和胸腔积液中CYFRA211、NSE及SCC水平变化及意义. 中国实用医刊. 2023(09): 34-37 .
9.	陈彪. 低剂量螺旋CT联合血清肿瘤标志物在肺癌诊断中的临床应用价值. 影像研究与医学应用. 2023(22): 24-26 .

Other cited types(1)

Get Citation

PDF

XML

Article views (1656) PDF downloads (644) Cited by(10)

Turn off MathJax

Article Contents

Abstract

References

Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu