| Citation: |
HAN Zheng, HUANG Xiaodong, LIU Meng, ZHU Qingxi, TAN Jie, LIU Weijie, CHEN Wei, ZOU Yanli, CAI Yishan, HUANG Shasha, TIAN Xia. Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu[J]. Cancer Research on Prevention and Treatment, 2019, 46(8): 696-701. DOI: 10.3971/j.issn.1000-8578.2019.18.1927
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To investigate the effect of Noscapine(Nos) on drug resistance of 5-Fu-resistant human colon cancer cell lines and its mechanism.
Drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu were established. MTT assay was used to test IC50 and cell morphology was observed. Cell cycle and apoptosis were determined by flow cytometry. RT-qPCR and Western blot were used to detect the expression and phosphorylation of P38 and the expression of drug resistance-related proteins.
The drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu were successfully constructed. Compared with control group, cell cycle of colon cancer drug-resistant cell lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu after Nos intervention was significantly blocked at G0/G1 phase (P < 0.01), the apoptosis rate was increased, the expression of P38 and phosphorylation were significantly inhibited (P < 0.01), and the expression of MRP, LRP and P-glycoprotein were significantly decreased (P < 0.01).
Noscapine has a certain toxic effect on 5-Fu-resistant human colon cancer cells and reduces drug resistance. The mechanism may be related to the inhibition of P38 expression and phosphorylation.
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