| Citation: |
CHEN Panli, TANG Jianrong, ZHANG Yiqiang. Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line[J]. Cancer Research on Prevention and Treatment, 2019, 46(8): 702-706. DOI: 10.3971/j.issn.1000-8578.2019.18.1867
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To investigate the effect of astragaloside A on the proliferation and migration of human colon cancer SW480 cell line.
The effect of astragaloside A on the viability of SW480 cells was detected by drug cytotoxicity test. The half inhibitory concentration was 168 μmol/L. Cell proliferation rate was detected by CCK-8. The expression levels of Ki67, PCNA, bcl-2, bax, cleaved caspase-3 and cleaved caspase-9 were detected by Western blot. The apoptosis of SW480 cell was detected by Hoechst staining.
The viability of SW480 cell was significantly decreased while the concentration of astragaloside was greater than 20 μmol/L. Compared with the Control group, the proliferation rate was significantly decreased after SW480 cells were treated with astragaloside A. There was no significant change in the expression level of Ki67 in the low-dose astragaloside A group. The expression levels of Ki67 and PCNA in the middle-dose and high-dose astragaloside A groups were significantly decreased. After the intervention of astragaloside A, the ratio of apoptotic cells was significantly increased, the expression level of bcl-2 was significantly decreased, the expression levels of bax, cleaved caspase-3 and cleaved caspase-9 were significantly increased, and the effect was more obvious with the increase of astragaloside A concentration.
Astragaloside A could inhibit the proliferation and induce the apoptosis of human colon cancer SW480 cell line.
| [1] |
Siegel RL, Miller KD, Fedewa SA, et al. Colorectal cancer statistics, 2017[J]. CA Cancer J Clin, 2017, 67(3): 117-193. http://d.old.wanfangdata.com.cn/Periodical/zhlxbx201701012
|
| [2] |
Afrin S, Giampieri F, Gasparrini M, et al. Chemopreventive and Therapeutic Effects of Edible Berries: A Focus on Colon Cancer Prevention and Treatment[J]. Molecules, 2016, 21(2): 169. doi: 10.3390/molecules21020169
|
| [3] |
Stein KB, Snyder CF, Barone BB, et al. Colorectal cancer outcomes, recurrence, and complications in persons with and without diabetes mellitus: a systematic review and meta-analysis [J]. Dig Dis Sci, 2010, 55(7): 1839-1851. doi: 10.1007/s10620-009-0944-8
|
| [4] |
André T, Boni C, Navarro M, et al. Improved overall survival with oxaliplatin, fluorouracil, and leucovorin as adjuvant treatment in stage Ⅱ or Ⅲ colon cancer in the mosaic trial [J]. J Clin Oncol, 2009, 27(19): 3109-3116. doi: 10.1200/JCO.2008.20.6771
|
| [5] |
杨庆珍, 王增绘, 付娟, 等.黄芪化学成分与生态因子的相关性[J].应用生态学报, 2015, 26(3): 732-738. http://d.old.wanfangdata.com.cn/Periodical/yystxb201503012
Yang QZ, Wang ZH, Fu J, et al. Correlation between chemical constituents and ecological factors of astragalus membranaceus var.mongholicus[J]. Ying Yong Sheng Tai Xue Bao, 2015, 26(3): 732-738. http://d.old.wanfangdata.com.cn/Periodical/yystxb201503012
|
| [6] |
Du M, Wu XJ, Ding J, et al. Astragaloside Ⅳ and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors[J]. Biotechnol Lett, 2003, 25(21): 1853-1856. doi: 10.1023/A:1026233728375
|
| [7] |
Ren S, Zhang H, Mu Y, et al. Pharmacological effects of Astragaloside Ⅳ: a literature review[J]. J Tradit Chin Med, 2013, 33(3): 413-416. doi: 10.1016/S0254-6272(13)60189-2
|
| [8] |
Gui D, Guo Y, Feng W, et al. Astragaloside Ⅳ, a novel antioxidant, prevents glucose-induced podocyte apoptosis in vitro and in vivo[J]. PLoS One, 2012, 7(6): e39824. doi: 10.1371/journal.pone.0039824
|
| [9] |
Xie T, Li Y, Li SL, et al. Astragaloside Ⅳ enhances cisplatin chemosensitivity in human colorectal cancer via regulating NOTCH3[J]. Oncol Res, 2016, 24(6): 447-453. doi: 10.3727/096504016X14685034103590
|
| [10] |
庞歆桥, 赵鹏飞, 杨凡, 等.黄芪甲苷联合西妥昔单抗对人结肠癌细胞系RKO自噬调节与增殖的抑制作用[J].实用医学杂志, 2016, 32(18): 2992-2995. doi: 10.3969/j.issn.1006-5725.2016.18.014
Pang XQ, Zhao PF, Yang F, et al. Astragaloside combine with Cetuximab inhibits proliferation and regulates autophagy of human colon cancer cell RKO[J]. Shi Yong Yi Xue Za Zhi, 2016, 32(18): 2992-2995. doi: 10.3969/j.issn.1006-5725.2016.18.014
|
| [11] |
Lin R, Zhang Z, Chen L, et al. Dihydroartemisinin (dha) induces ferroptosis and causes cell cycle arrest in head and neck carcinoma cells[J]. Cancer Lett, 2016, 381(1): 165-175. doi: 10.1016/j.canlet.2016.07.033
|
| [12] |
喻春皓, 王冲之, 袁钧苏.西洋参抗癌研究进展——以结直肠癌为研究范例[J].药学学报, 2013, 48(7): 986-992. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yxxb201307005
Yu CH, Wang CZ, Yuan CS. Progress in anti-cancer research of american ginseng: With an example of colorectal cancer[J]. Yao Xue Xue Bao, 2013, 48(7): 986-992. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yxxb201307005
|
| [13] |
Zhang A, Zheng Y, Que Z, et al. Astragaloside Ⅳ inhibits progression of lung cancer by mediating immune function of Tregs and CTLs by interfering with IDO[J]. J Cancer Res Clin Oncol, 2014, 140(11): 1883-1890. doi: 10.1007/s00432-014-1744-x
|
| [14] |
Jiang K, Lu Q, Li Q, et al. Astragaloside Ⅳ inhibits breast cancer cell invasion by suppressing vav3 mediated rac1/mapk signaling [J]. Int Immunopharmacol, 2017, 42: 195-202. doi: 10.1016/j.intimp.2016.10.001
|
| [15] |
Ou Yang Y, Huang J, Ouyang Z, et al. Enrichment and purification process of astragalosides and their anti-human gastric cancer MKN-74 cell proliferation effect[J]. Afr Health Sci, 2014, 14(1): 22-27. doi: 10.4314/ahs.v14i1.5
|
| [16] |
马鹏飞, 阮柏, 王德盛, 等.黄芪甲苷对肝癌细胞增殖和侵袭能力的影响及其机制研究[J].现代肿瘤医学, 2014, 22(5): 1013-1015. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sxzlyx201405010
The anti-proliferation and anti-invasion effect of astragaloside Ⅳ on liver cancer cell line in vitro and its mechanism[J]. Xian Dai Zhong Liu Yi Xue, 2014, 22(5): 1013-1015. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sxzlyx201405010
|
| [17] |
邓樱, 陈红风.黄芪注射液及其有效成分对乳腺癌细胞增殖和Akt磷酸化的影响[J].中西医结合学报, 2009, 7(12): 1174-1180. http://d.old.wanfangdata.com.cn/Periodical/zxyjhxb200912016
Deng Y, Chen HF. Effects of Astragalus injection and its ingredients on proliferation and Akt phosphorylation of breast cancer cell lines[J]. Zhong Xi Yi Jie He Xue Bao, 2009, 7(12): 1174-1180. http://d.old.wanfangdata.com.cn/Periodical/zxyjhxb200912016
|
| [18] |
Juríková M, Danihel Ľ, Polák Š, et al. Ki67, PCNA, and MCM proteins: markers of proliferation in the diagnosis of breast cancer [J]. Acta Histochem, 2016, 118(5): 544-552. doi: 10.1016/j.acthis.2016.05.002
|
| [19] |
Strzalka W, Ziemienowicz A. Proliferating cell nuclear antigen (pcna): A key factor in DNA replication and cell cycle regulation [J]. Ann Bot, 2011, 107(7): 1127-1140. doi: 10.1093/aob/mcq243
|
| [20] |
Yan L, Yu HH, Liu YS, et al. Esculetin enhances the inhibitory effect of 5-fluorouracil on the proliferation, migration and epithelial-mesenchymal transition of colorectal cancer[J]. Cancer Biomark, 2019, 24(2): 231-240. doi: 10.3233/CBM-181764
|
| [21] |
Mirzayans R, Andrais B, Kumar P, et al. The growing complexity of cancer cell response to DNA-Damaging agents: Caspase 3 mediates cell death or survival?[J]. Int J Mol Sci, 2016, 17(5). pii: E708. doi: 10.3390/ijms17050708
|
| [22] |
杨雨, 徐文娟, 彭康, 等.穗花杉双黄酮通过影响caspase-3和β-catenin表达诱导结肠癌细胞SW480凋亡[J].南方医科大学学报, 2014, 34(7): 1035-1038. doi: 10.3969/j.issn.1673-4254.2014.07.24
Yang Y, Xu WJ, Peng K, et al. Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulating β-catenin and caspase-3 expressions[J]. Nan Fang Yi Ke Da Xue Xue Bao, 2014, 34(7): 1035-1038. doi: 10.3969/j.issn.1673-4254.2014.07.24
|
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