Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line

CHEN Panli; TANG Jianrong; ZHANG Yiqiang

doi:10.3971/j.issn.1000-8578.2019.18.1867

Cancer Research on Prevention and Treatment > 2019 > 46(8): 702-706. > DOI: 10.3971/j.issn.1000-8578.2019.18.1867

CHEN Panli, TANG Jianrong, ZHANG Yiqiang. Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line[J]. Cancer Research on Prevention and Treatment, 2019, 46(8): 702-706. DOI: 10.3971/j.issn.1000-8578.2019.18.1867

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Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line

Department of Gastroenterology, Zhumadian Central Hospital, Zhumadian 463000, China

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Received Date: December 04, 2018
Revised Date: March 14, 2019
Available Online: January 14, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of astragaloside A on the proliferation and migration of human colon cancer SW480 cell line.
Methods
The effect of astragaloside A on the viability of SW480 cells was detected by drug cytotoxicity test. The half inhibitory concentration was 168 μmol/L. Cell proliferation rate was detected by CCK-8. The expression levels of Ki67, PCNA, bcl-2, bax, cleaved caspase-3 and cleaved caspase-9 were detected by Western blot. The apoptosis of SW480 cell was detected by Hoechst staining.
Results
The viability of SW480 cell was significantly decreased while the concentration of astragaloside was greater than 20 μmol/L. Compared with the Control group, the proliferation rate was significantly decreased after SW480 cells were treated with astragaloside A. There was no significant change in the expression level of Ki67 in the low-dose astragaloside A group. The expression levels of Ki67 and PCNA in the middle-dose and high-dose astragaloside A groups were significantly decreased. After the intervention of astragaloside A, the ratio of apoptotic cells was significantly increased, the expression level of bcl-2 was significantly decreased, the expression levels of bax, cleaved caspase-3 and cleaved caspase-9 were significantly increased, and the effect was more obvious with the increase of astragaloside A concentration.
Conclusion
Astragaloside A could inhibit the proliferation and induce the apoptosis of human colon cancer SW480 cell line.
- Colon cancer,
- Astragaloside A,
- Proliferation,
- Apoptosis

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References (22)

References

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Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line