| Citation: |
ZHAO Tianzeng, YANG Jinhua, LIU Xiangqian, XU Mengbo. miR-145 Attenuates Migration and Invasion of Non-small Cell Lung Cancer A549 Cells by Targeting AP4[J]. Cancer Research on Prevention and Treatment, 2019, 46(2): 110-114. DOI: 10.3971/j.issn.1000-8578.2019.18.1160
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To investigate the effect of miR-145 on the migration and invasion of non-small cell lung cancer (NSCLC) cells and possible mechanism.
The expression of miR-145 and activating enhancer binding protein 4 (AP4) mRNA in NSCLC tissues were detected by qRT-PCR. After the transfection with miR-145 mimic or AP4 siRNA, the migration and invasion of A549 cells were examined by wound healing and Transwell assay, respectively; The mRNA and protein expressions of AP4 were detected by qRT-PCR and Western blot, respectively. The relationship between AP4 mRNA and miR-145 was detected by double luciferase reporter gene method.
Compared with adjacent normal tissues, the level of miR-145 was significantly decreased in NSCLC tissues (P < 0.05), however, the mRNA and protein levels of AP4 were significantly increased (P < 0.05). Compared with control group, the level of miR-145 was significantly increased (P < 0.05), the mRNA and protein levels of AP4 were significantly decreased (P < 0.05), and cells migration and invasion were significantly decreased (P < 0.05) in A549 cells after the transfection with miR-145 mimic. Moreover, AP4 was a direct target of miR-145. After the transfection with AP4 siRNA, the migration and invasion of A549 cells were significantly decreased (P < 0.05).
miR-145 upregulation could efficiently attenuate the migration and invasion of A549 cells via inhibiting target gene AP4 expression.
| [1] |
Ferlay J, Soerjomataram I, Dikshit R, et al.Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012[J].Int J Cancer, 2015, 136(5):E359-86. doi: 10.1002/ijc.29210
|
| [2] |
Balça-Silva J, Sousa Neves S, Gonçalves AC, et al.Effect of miR-34b overexpression on the radiosensitivity of non-small cell lung cancer cell lines[J].Anticancer Res, 2012, 32(5):1603-9. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=9e553c61fbb76e70300e33fa0770d47b
|
| [3] |
Ostenfeld MS, Bramsen JB, Lamy P, et al.miR-145 induces caspase-dependent and independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Tabladder tumors[J].Oncologene, 2010, 29(7):1073-84. http://onlinelibrary.wiley.com/resolve/reference/PMED?id=19915607
|
| [4] |
孙嘉伟, 刘凤霞, 刘文雅, 等.MicroRNA-145对食管鳞状细胞癌细胞增殖的影响[J].新疆医科大学学报, 2013, 36(6):756-8, 762. http://d.old.wanfangdata.com.cn/Periodical/xjykdxxb201306009
Sun JW, Liu FX, Liu WY, et al.The effect of microRNA-145 on proliferation of esophageal squamous carcinoma cells[J].Xinjiang Yi Ke Da Xue Xue Bao, 2013, 36(6):756-8, 762. http://d.old.wanfangdata.com.cn/Periodical/xjykdxxb201306009
|
| [5] |
Ding W, Tan H, Zhao C, et al.MiR-145 suppresses cell proliferation and motility by inhibiting ROCK1 in hepatocellular carcinoma[J].Tumour Biol, 2016, 37(5):6255-60. doi: 10.1007/s13277-015-4462-3
|
| [6] |
Gao P, Xing AY, Zhou GY, et al.The molecular mechanism of microRNA-145 to suppress invasion-metastasis cascade in gastric cancer[J].Oncogene, 2013, 32(4):491-501. doi: 10.1038/onc.2012.61
|
| [7] |
Sachdeva M, Mo YY.MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1[J].Cancer Res, 2010, 70(1):378-87. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=bfdcdb00c6ee02b3195275e1c346be0f
|
| [8] |
王寰昱, 王亚峰, 张昆松, 等.上调miRNA-145对肝癌细胞的作用及其机制研究[J].中国病理生理杂志, 2015, 31(6):1019-25. http://www.cnki.com.cn/Article/CJFDTotal-ZBLS201506011.htm
Wang HY, Wang YF, Zhang KS, et al.Role of up-regulated microRNA145 in viability, apoptosis, invasion and metastasis of hepatoma cells[J].Zhongguo Bing Li Sheng Li Za Zhi, 2015, 31(6):1019-25. http://www.cnki.com.cn/Article/CJFDTotal-ZBLS201506011.htm
|
| [9] |
孙政, 曹杰, 杨平, 等.转录因子激活蛋白-4在结直肠癌中的表达及与细胞凋亡的相关性[J].中华实验外科杂志, 2008, 25(7):891-3. doi: 10.3321/j.issn:1001-9030.2008.07.028
Sun Z, Cao J, Yang P, et al.Expression of activator protein-4 in colorectal carcinoma and its relationship with apoptosis[J].Zhonghua Shi Yan Wai Ke Za Zhi, 2008, 25(7):891-3. doi: 10.3321/j.issn:1001-9030.2008.07.028
|
| [10] |
Jung P, Hermeking H.The c-MYC-AP4-p21 cascade[J].Cell Cycle, 2009, 8(7):982-9. doi: 10.4161/cc.8.7.7949
|
| [11] |
Hu X, Guo W, Chen S, et al.Silencing of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human lung cancer cells[J].Oncol Lett, 2016, 11(6):3735-42. doi: 10.3892/ol.2016.4451
|
| [12] |
Xinghua L, Bo Z, Yan G, et al.The overexpression of AP-4 as a prognostic indicator for gastric carcinoma[J].Med Oncol, 2012, 29(2):871-7. doi: 10.1007/s12032-011-9845-8
|
| [13] |
Hu BS, Zhao G, Yu HF, et al.High expression of AP-4 predicts poor prognosis for hepatocellular carcinoma after curative hepatectomy[J].Tumour Biol, 2013, 34(1):271-6. doi: 10.1007/s13277-012-0547-4
|
| [14] |
王小康, 解绪红, 陈文全, 等.转录因子激活蛋白4、血管内皮生长因子C在甲状腺乳头癌中的表达及意义[J].中华普通外科学文献(电子版), 2013, 7(1):36-9. http://d.old.wanfangdata.com.cn/Periodical/zhptwkxwx201301009
Wang XK, Xie XH, Chen WQ, et al.Expression and clinical values of AP-4 and VEGF-C in papillary carcinoma of thyroid[J].Zhonghua Pu Tong Wai Ke Xue Wen Xian (Dian Zi Ban), 2013, 7(1):36-9. http://d.old.wanfangdata.com.cn/Periodical/zhptwkxwx201301009
|
| [15] |
别延红, 李纳, 吴又明, 等.转录因子增强结合蛋白-4基因沉默对子宫内膜癌细胞凋亡的影响[J].山东医药, 2017, 57(2):5-9. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=shandyy201702002
Bie YH, Li N, Wu YM, et al.Effects of silencing transcription factor AP-4 gene on apoptosis of endometrial cancer cells[J].Shandong Yi Yao, 2017, 57(2):5-9. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=shandyy201702002
|
| [16] |
Chen S, Chiu SK.AP4 activates cell migration and EMT mediated by p53 in MDA-MB-231 breast carcinoma cells[J].Mol Cell Biochem, 2015, 407(1-2):57-68. doi: 10.1007/s11010-015-2454-7
|
| [17] |
Jackstadt R, Röh S, Neumann J, et al.AP4 is a mediator of epithelial-mesenchymal transition and metastasis in colorectal cancer[J].J Exp Med, 2013, 210(7):1331-50. doi: 10.1084/jem.20120812
|
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