Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer

ZHANG Meng; MA Dong; YUAN Teng; FAN Shaobei; GUO Ying; LIU Jia; LI Ou

doi:10.3971/j.issn.1000-8578.2019.18.1040

Cancer Research on Prevention and Treatment > 2019 > 46(4): 338-344. > DOI: 10.3971/j.issn.1000-8578.2019.18.1040

ZHANG Meng, MA Dong, YUAN Teng, FAN Shaobei, GUO Ying, LIU Jia, LI Ou. Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer[J]. Cancer Research on Prevention and Treatment, 2019, 46(4): 338-344. DOI: 10.3971/j.issn.1000-8578.2019.18.1040

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Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer

1.
Graduate School of North China University of Science and Technology, Tangshan 063210, China
2.
Public Health School of North China University of Science and Technology, Tangshan 063210, China
3.
Medical Experiment Center Jitang College of North China University of Science and Technology, Tangshan 063210, China
4.
Department of Obstetrics and Gynecology, Tangshan Gongren Hospital, Tangshan 063000, China

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Corresponding author:
LI Ou, E-mail: tsliou@163.com
Received Date: August 21, 2018
Revised Date: November 28, 2018
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the expressions of miR-135a-5p, GATA3 and STAT3 in different pathological types of cervical tissues and cervical cancer cell lines and their correlation in cervical cancer.
Methods
Real-time PCR was performed to determine the expression of miR-135a-5p in cervical carcinoma tissue and cell lines. Immunohistochemistry SP and Western blot methods were used to determine the expression of GATA3 and STAT3 in cervical tissues and cells. The effect of miR-135a-5p on GATA3 3'UTR was verified by dual-luciferase reporter assay system. The correlation of miR-135a-5p and GATA3 mRNA expression with the clinical pathological parameters of cervical squamous cell carcinoma patients was analyzed.
Results
The miR-135a-5p expression was up-regulated in CSCC tissues and cell lines(all P < 0.05). With the increasing stage of carcinoma, the mRNA levels of GATA3 was reduced(P < 0.05), whereas the mRNA levels of STAT3 was increased(P < 0.05). miR-135a-5p could target GATA3 3'UTR. The expression of GATA3 mRNA was negatively correlated with STAT3 mRNA expression(r=-0.4534) and miR-135a-5p expression(r=-0.6656). Moreover, the miR-135a-5p expression was related to clinical stages and lymph node metastasis(P < 0.05), and the GATA3 mRNA expression was related to clinical stage, depth of invasion and lymph node metastasis in cervical squamous cell carcinoma(P < 0.05).
Conclusion
In cervical cancer, the expression of miR-135a-5p and STAT3 mRNA are up-regulated, the expression of GATA3 mRNA is down-regulated, suggesting that they may be involved in the occurrence and development of cervical cancer through interaction.
- Cervical cancer,
- MicroRNA-135a-5p,
- GATA-binding protein 3,
- Signal transduction and transcription 3

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References (19)

References

[1]	Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015[J]. CA Cancer J Clin, 2015, 65(1): 5-29. doi: 10.3322/caac.21254
[2]	González-Quintana V, Palma-Berré L, Campos-Parra AD, et al. MicroRNAs are involved in cervical cancer development, progression, clinical outcome and improvement treatment response (Review)[J]. Oncol Rep, 2016, 35(1): 3-12. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=b14c76d7fe337e27c3f2bb2de3c36d6d
[3]	Zhou H, Guo W, Zhao Y, et al. MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer[J]. Cancer Sci, 2014, 105(8): 956-65. doi: 10.1111/cas.2014.105.issue-8
[4]	Müller S, Nowak K. Exploring the miRNA-mRNA Regulatory Network in Clear Cell Renal Cell Carcinomas by Next-Generation Sequencing Expression Profiles[J]. Biomed Res Int, 2014, 2014: 948408.
[5]	Guo LM, Ding GF, Xu WC, et al. MiR-135a-5p represses proliferation of HNSCC by targeting HOXA10[J]. Cancer Biol Ther, 2018, 1-28. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1080/15384047.2018.1450112
[6]	Yang Z, He L, Lin K, et al. The KMT1A-GATA3-STAT3 circuit is a novel self-renewal signaling of human bladder cancer stem cells[J]. Clin Cancer Res, 2017, 23(21): 6673-85. doi: 10.1158/1078-0432.CCR-17-0882
[7]	Yao S, Tian C, Ding Y, et al. Down-regulation of Krüppel-like factor-4 by microRNA-135a-5p promotes proliferation and metastasis in hepatocellular carcinoma by transforming growth factor-β1[J]. Oncotarget, 2016, 7(27): 42566-78.
[8]	Pan YM, Wang CG, Zhu M, et al. STAT3 signaling drives EZH2 transcriptional activation and mediates poor prognosis in gastric cancer[J]. Mol Cancer, 2016, 15(1): 79. doi: 10.1186/s12943-016-0561-z
[9]	Yuan J, Zhang F, Niu R. Multiple regulation pathways and pivotal biological functions of STAT3 in cancer[J]. Sci Rep, 2015, 5: 17663.
[10]	Pedroza-Torres A, López-Urrutia E, García-Castillo V, et al. MicroRNAs in cervical cancer: evidences for a miRNA profile deregulated by HPV and its impact on radio-resistance[J]. Molecules, 2014, 19(5): 6263-81. doi: 10.3390/molecules19056263
[11]	Kouros-Mehr H, Bechis SK, Slorach EM, et al. GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model[J]. Cancer Cell, 2008, 13(2): 141-52. doi: 10.1016/j.ccr.2008.01.011
[12]	Dydensborg AB, Rose AA, Wilson BJ, et al. GATA3 inhibits breast cancer growth and pulmonary breast cancer metastasis[J]. Oncogene, 2009, 28(29): 2634-42. doi: 10.1038/onc.2009.126
[13]	Chou J, Provot S, Werb Z. GATA3 in Development and Cancer Differentiation: Cells GATA Have It![J]. J Cell Physiol, 2010, 222(1): 42-9. doi: 10.1002/jcp.v222:1
[14]	Yoon NK, Maresh EL, Shen D, et al. Higher Levels of GATA3 Predict Better Survival in Women with Breast Cancer[J]. Hum Pathol, 2010, 41(12): 1794-801. doi: 10.1016/j.humpath.2010.06.010
[15]	Li Y, Ishiguro H, Kawahara T, et al. Loss of GATA3 in bladder cancer promotes cell migration and invasion[J]. Cancer Biol Ther, 2014, 15(4): 428-35. doi: 10.4161/cbt.27631
[16]	Yu H, Lee H, Herrmann A, et al. Revisiting STAT3 signalling in cancer: new and unexpected biological functions[J]. Nat Rev Cancer, 2014, 14(11): 736-46. doi: 10.1038/nrc3818
[17]	Geiger JL, Grandis JR, Bauman JE. The STAT3 pathway as a therapeutic target in head and neck cancer: Barriers and innovations[J]. Oral Oncol, 2016, 56(28): 84-92. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1177/0272989X080280010103
[18]	Mumm JB, Emmerich J, Zhang X, et al. IL-10 elicits IFNγ-dependent tumor immune surveillance[J]. Cancer Cell, 2011, 20(6): 781-96. doi: 10.1016/j.ccr.2011.11.003
[19]	Ma D, Jiang C, Hu X, et al. Methylation Patterns of the IFN-γ Gene in Cervical Cancer Tissues[J]. Sci Rep, 2014, 4(7545): 6331.

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Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer