| Citation: |
DU Xiaoqin. Expression and Clinical Significance of LncRNA KCNQ1OT1 in Ovarian Cancer Tissues[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004
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To explore the expression of LncRNA KCNQ1 overlapping transcript 1 (KCNQ1OT1) in ovarian cancer tissues and its relationship with clinicopathological features.
The expression levels of LncRNA KCNQ1OT1 in ovarian cancer tissues, adjacent normal tissues and ovarian cancer cell lines were assessed by quantitative real-time PCR.Then, the associations of LncRNA KCNQ1OT1 expression levels with clinicopathological features and prognosis were evaluated. In addition, LncRNA KCNQ1OT1 functions in tumor progression and invasion were further determined by siRNA-induced LncRNA KCNQ1OT1 silencing in vitro.
The expression levels of LncRNA KCNQ1OT1 in ovarian cancer tissues and cell lines were significantly higher than those in adjacent non-tumor tissues and normal ovarian epithelial cells. Higher LncRNA KCNQ1OT1 expression levels were associated with a shorter overall survival (P=0.006) and disease-free survival (P=0.001) in ovarian cancer patients. In addition, LncRNA KCNQ1OT1 expression was positively correlated with FIGO stage (P=0.002), tumor grade (P=0.006) and distant metastasis (P < 0.001). Moreover, LncRNA KCNQ1OT1 knockdown in ovarian cancer cells markedly suppressed cell proliferation and invasion.
The LncRNA KCNQ1OT1 is a novel factor involved in ovarian cancer progression, and constitutes a potential prognostic biomarker and therapeutic target for the patients with ovarian cancer.
| [1] |
Chai Y, Liu J, Zhang Z, et al.HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer[J].Cancer Med, 2016, 5(7): 1588-98. doi: 10.1002/cam4.2016.5.issue-7
|
| [2] |
Chen Q, Liu X, Xu L, et al.Long non-coding RNA BACE1-AS is a novel target for anisomycin-mediated suppression of ovarian cancer stem cell proliferation and invasion[J].Oncol Rep, 2016, 35(4): 1916-24. https://www.ncbi.nlm.nih.gov/gtr/genes/100379571/
|
| [3] |
Ren P, Yang XQ, Zhai XL, et al.Overexpression of Rab27B is correlated with distant metastasis and poor prognosis in ovarian cancer[J].Oncol Lett, 2016, 12(2): 1539-45. https://www.researchgate.net/publication/304608555_Overexpression_of_Rab27B_is_correlated_with_distant_metastasis_and_poor_prognosis_in_ovarian_cancer
|
| [4] |
Hong HH, Hou LK, Pan X, et al.Long non-coding RNA UCA1 is a predictive biomarker of cancer[J].Oncotarget, 2016, 7(28): 44442-7. https://www.researchgate.net/publication/304326313_Long_non-coding_RNA_UCA1_is_a_predictive_biomarker_of_cancer
|
| [5] |
Hu L, Chen SH, Lv QL, et al.Clinical Significance of Long Non-Coding RNA CASC8 rs10505477 Polymorphism in Lung Cancer Susceptibility, Platinum-Based Chemotherapy Response, and Toxicity[J].Int J Environ Res Public Health, 2016, 13(6): pii:E545. doi: 10.3390/ijerph13060545
|
| [6] |
Nakano S, Murakami K, Meguro M, et al.Expression profile of LIT1/KCNQ1OT1 and epigenetic status at the KvDMR1 in colorectal cancers[J].Cancer Sci, 2006, 97(11): 1147-54. doi: 10.1111/cas.2006.97.issue-11
|
| [7] |
Soejima H, Nakagawachi T, Zhao W, et al.Silencing of imprinted CDKN1C gene expression is associated with loss of CpG and histone H3 lysine 9 methylation at DMR-LIT1 in esophageal cancer[J].Oncogene, 2004, 23(25): 4380-8. doi: 10.1038/sj.onc.1207576
|
| [8] |
Wan J, Huang M, Zhao H, et al.A novel tetranucleotide repeat polymorphism within KCNQ1OT1 confers risk for hepatocellular carcinoma[J].DNA Cell Biol, 2013, 32(11): 628-34. doi: 10.1089/dna.2013.2118
|
| [9] |
Meryet-Figuiere M, Lambert B, Gauduchon P, et al.An overview of long non-coding RNAs in ovarian cancers[J].Oncotarget, 2016, 7(28): 44719-34. https://www.researchgate.net/publication/298908621_An_overview_of_long_non-coding_RNAs_in_ovarian_cancers
|
| [10] |
Araujo ES, Vasques LR, Stabellini R, et al.Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line[J].Braz J Med Biol Res, 2014, 47(12): 1029-35. doi: 10.1590/1414-431X20144058
|
| [11] |
Wang SH, Ma F, Tang ZH, et al.Long non-coding RNA H19 regulates FOXM1 expression by competitively binding endogenous miR-342-3p in gallbladder cancer[J].J Exp Clin Cancer Res, 2016, 35(1): 160. doi: 10.1186/s13046-016-0436-6
|
| [12] |
Hu L, Lv QL, Chen SH, et al.Up-Regulation of Long Non-Coding RNA AB073614 Predicts a Poor Prognosis in Patients with Glioma[J].Int J Environ Res Public Health, 2016, 13(4): 433. doi: 10.3390/ijerph13040433
|
| [13] |
Huang S, Qing C, Huang Z, et al.The long non-coding RNA CCAT2 is up-regulated in ovarian cancer and associated with poor prognosis[J].Diagn Pathol, 2016, 11(1): 49. doi: 10.1186/s13000-016-0499-x
|
| [14] |
Jiang P, Wu X, Wang X, et al.NEAT1 upregulates EGCG-induced CTR1 to enhance cisplatin sensitivity in lung cancer cells[J].Diagn Pathol, 2016, 7(28): 43337-51. https://www.researchgate.net/publication/303877326_NEAT1_upregulates_EGCG-induced_CTR1_to_enhance_cisplatin_sensitivity_in_lung_cancer_cells
|
| [15] |
Ozes AR, Miller DF, Ozes ON, et al.NF-kappaB-HOTAIR axis links DNA damage response, chemoresistance and cellular senescence in ovarian cancer[J].Oncogene, 2016, 35(41): 5350-61. doi: 10.1038/onc.2016.75
|
| [16] |
Yang P, Chen T, Xu Z, et al.Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO[J].Oncotarget, 2016, 7(27): 42194. https://www.researchgate.net/publication/303798131_Long_noncoding_RNA_GAPLINC_promotes_invasion_in_colorectal_cancer_by_targeting_SNAI2_through_binding_with_PSF_and_NONO
|
| [17] |
XianGuo C, ZongYao H, Jun Z, et al.Promoting progression and clinicopathological significance of NEAT1 over-expression in bladder cancer[J].Oncotarget, 2016, [Epub ahead of print].
|
| [18] |
Liu T, Zhang X, Yang YM, et al.Increased expression of the long noncoding RNA CRNDE-h indicates a poor prognosis in colorectal cancer, and is positively correlated with IRX5 mRNA expression[J].Onco Targets Ther, 2016, 9: 1437-48. https://www.ncbi.nlm.nih.gov/pmc/?term=incrna+cancer&cmd=DetailsSearch
|
| [19] |
Huang NS, Chi YY, Xue JY, et al.Long non-coding RNA metastasis associated in lung adenocarcinoma transcript 1(MALAT1) interacts with estrogen receptor and predicted poor survival in breast cancer[J].Oncotarget, 2016, 7(25): 37957-65. https://www.researchgate.net/profile/Naisi_Huang/publication/303403296_Long_non-coding_RNA_metastasis_associated_in_lung_adenocarcinoma_transcript_1_MALAT1_interacts_with_estrogen_receptor_and_predicted_poor_survival_in_breast_cancer/links/5749a9c808ae5bf2e63f1564.pdf?origin=publication_detail
|
| [20] |
Zhou C, Ye L, Jiang C, et al.Long noncoding RNA HOTAIR, a hypoxia-inducible factor-1alpha activated driver of malignancy, enhances hypoxic cancer cell proliferation, migration, and invasion in non-small cell lung cancer[J].Tumour Biol, 2015, 36(12): 9179-88. doi: 10.1007/s13277-015-3453-8
|
| [21] |
Qiu M, Xu Y, Yang X, et al.CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer[J].Tumour Biol, 2014, 35(6): 5375-80. doi: 10.1007/s13277-014-1700-z
|
| [22] |
Shi X, Sun M, Liu H, et al.A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer[J].Mol Carcinog, 2015, 54 Suppl 1: E1-E12. https://www.sigmaaldrich.com/catalog/papers/24357161
|
| [23] |
Qiu H, Wang X, Guo R, et al.HOTAIR rs920778 polymorphism is associated with ovarian cancer susceptibility and poor prognosis in a Chinese population[J].Future Oncol, 2016, 13(4): 347-55. doi: 10.2217/pme-2016-0014
|
| [24] |
Zhang X, Li S, Dong C, et al.Knockdown of long noncoding RNA NR_026689 inhibits proliferation and invasion and increases apoptosis in ovarian carcinoma HO-8910PM cells[J].Oncol Res, 2017, 25(2): 259-65. doi: 10.3727/096504016X14732503870766
|
| [25] |
Zou A, Liu R, Wu X.Long non-coding RNA MALAT1 is up-regulated in ovarian cancer tissue and promotes SK-OV-3 cell proliferation and invasion[J].Neoplasma, 2016, 63(6): 865-72. doi: 10.4149/neo_2016_605
|
| [26] |
Li J, Yu H, Xi M, et al.Long noncoding RNA C17orf91 is a potential prognostic marker and functions as an oncogene in ovarian cancer[J].J Ovarian Res, 2016, 9(1): 49. doi: 10.1186/s13048-016-0258-3
|
| [27] |
Liu E, Liu Z, Zhou Y, et al.Overexpression of long non-coding RNA PVT1 in ovarian cancer cells promotes cisplatin resistance by regulating apoptotic pathways[J].Int J Clin Exp Med, 2015, 8(11): 20565-72. doi: 10.1007/s13277-016-5125-8
|
| [28] |
Schultz BM, Gallicio GA, Cesaroni M, et al.Enhancers compete with a long non-coding RNA for regulation of the Kcnq1 domain[J].Nucleic Acids Res, 2015, 43(2): 745-59. doi: 10.1093/nar/gku1324
|
| [29] |
Sunamura N, Ohira T, Kataoka M, et al.Regulation of functional KCNQ1OT1 lncRNA by beta-catenin[J].Sci Rep, 2016, 6: 20690. doi: 10.1038/srep20690
|
| [30] |
Bliek J, Maas SM, Ruijter JM, et al.Increased tumour risk for BWS patients correlates with aberrant H19 and not KCNQ1OT1 methylation: occurrence of KCNQ1OT1 hypomethylation in familial cases of BWS[J].Hum Mol Genet, 2001, 10(5): 467-76. doi: 10.1093/hmg/10.5.467
|
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