Advanced Search
LI Xiaojie, YIN Guilin, DONG Yongqiang, ZHU Shuibo, ZHANG Xiaoming, JI Tao, XU Jiahang. Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 320-323. DOI: 10.3971/j.issn.1000-8578.2017.05.002
Citation: LI Xiaojie, YIN Guilin, DONG Yongqiang, ZHU Shuibo, ZHANG Xiaoming, JI Tao, XU Jiahang. Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 320-323. DOI: 10.3971/j.issn.1000-8578.2017.05.002

Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance

More Information
  • Corresponding author:

    YIN Guilin, E-mail: yingl1952@163.com

  • Received Date: October 16, 2016
  • Revised Date: January 12, 2017
  • Available Online: January 12, 2024
  • Objective 

    To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues.

    Methods 

    There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry.

    Results 

    SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P < 0.01). The positive expression rate of SFRP1 protein in tumor tissues was significantly lower than that in adjacent tissues (38.3%(23/60) vs. 83.3%(25/30), (P < 0.001)). SFRP1 protein expression was correlated with differentiation degree, clinical stage and lymph node metastasis (P < 0.05). SFRP1 gene methylation was closely correlated with SFRP1 protein expression deficiency in lung adenocarcinoma tissues (r=-0.585, P < 0.001).

    Conclusion 

    The expression of SFRP1 gene methylation and protein are associated with the degree of differentiation and lymph node metastasis. The decrease of SFRP1 protein expression may be due to SFRP1 gene methylation.

  • [1]
    Fossat N, Jones V, Khoo PL, et al.Stringent requirement of a proper level of canonical Wnt signalling activity for head formation in mouse embryo[J].Development, 2011, 138(4): 667-76. doi: 10.1242/dev.052803
    [2]
    朱智杰, 阮君山, 李尧, 等.Wnt信号通路诱导肿瘤细胞上皮-间质转化的研究进展[J].中国药理学通报, 2012, 28(7): 904-7. http://www.cnki.com.cn/Article/CJFDTOTAL-YAOL201207006.htm

    Zhu ZJ, Ruan JS, Li X, et al.Research progress of Wnt signaling pathway induced EMT in tumor cells[J].Zhongguo Yao Li Xue Tong Bao, 2012, 28(7): 904-7. http://www.cnki.com.cn/Article/CJFDTOTAL-YAOL201207006.htm
    [3]
    Rawson JB, Manno M, Mrkonjic M, et al.Promoter methylation of Wnt antagonists DKK1 and SFRP1 is associated with opposing tumor subtypes in two large populations of colorectal cancer patients[J].Carcinogenesis, 2011, 32(5): 741-7. doi: 10.1093/carcin/bgr020
    [4]
    Jeong YJ, Jeong HY, Bong JG, et al.Low methylation levels of the SFRP1 gene are associated with the basal-ike subtype of breast cancer[J].Oncol Rep, 2013, 29(5): 1946-54. doi: 10.3892/or.2013.2335/download
    [5]
    Lin YW, Chung MT, Lai HC, et al.Methylation analysis of SFRP genes family in cervical adenocarcinoma[J].J Cancer Res Clin Oncol, 2009, 135(12): 1665-74. doi: 10.1007/s00432-009-0613-5
    [6]
    Ghoshal A, Ghosh SS.Expression, purification, and therapeutic implications of recombinant SFRP1[J].Appl Biochem Biotechnol, 2015, 175(4): 2087-103. doi: 10.1007/s12010-014-1354-8
    [7]
    Zhang H, Zhu YQ, Wu YQ, et al.Detection of promoter hypermethylation of Wnt antagonist genes in fecal samples for diagnosis of early colorectalcan[J].World J Gastroenterol, 2014, 20(20): 6329-35. doi: 10.3748/wjg.v20.i20.6329
    [8]
    Ricketts CJ, Hill VK, Linehan WM.Tumor-specific hypermethylation of epigenetic biomarkers, including SFRP1, predicts for poorer survival in patients from the TCGA Kidney Renal Clear Cell Carcinoma (KIRC) project[J].PLoS One, 2014, 9(1): e85621. doi: 10.1371/journal.pone.0085621
    [9]
    Licchesi JD, Westra WH, Hooker CM, et al.Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung[J].Carcinogenesis, 2008, 29(5): 895-904. doi: 10.1093/carcin/bgn017
    [10]
    方汉林, 于在诚, 祝会斌.SFRP1基因甲基化及其蛋白在非小细胞肺癌组织中的表达[J].中国肿瘤外科杂志, 2012, 4(2): 95-8. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLWK201202010.htm

    Fang HL, Yu ZC, Zhu HB.Expression of SFRPl genemethylation and SFRPl protein in non-small cell lung cancer[J].Zhongguo Zhong Liu Wai Ke Za Zhi, 2012, 4(2): 95-8. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLWK201202010.htm
    [11]
    Bremnes RM, Veve R, Gabrielson E, et al.High-throughput tissue micro-array analysis used to evaluate biology and prognostic significance of the E-cadherin pathway in non-small-cell lung cancer[J].J Clin Oncol, 2002, 20(10): 2417-28. doi: 10.1200/JCO.2002.08.159
    [12]
    Pirinen RT, Hirvikoski P, Johansson RT, et al.Reduced expression of alpha-catenin, beta-catenin, and gamma-catenin is associated with high cell proliferative activity and poor differentiation in non-small cell lung cancer[J].J Clin Pathol, 2001, 54(5): 391-5. doi: 10.1136/jcp.54.5.391
    [13]
    Taquchi YH, Iwadate M, Umeyama H.SFRP1 is a possible candidate for epigenetic therapy in non-small cell lung cancer[J].BMC Med Genomics, 2016, 9 Suppl 1: 28. https://www.researchgate.net/profile/Y_H_Taguchi/publication/306084951_SFRP1_is_a_possible_candidate_for_epigenetic_therapy_in_non-small_cell_lung_cancer/links/57b003ad08ae0101f1776d7f.pdf?origin=publication_list
  • Related Articles

    [1]XIAO Jincheng, GUO Leiming, KANG Xinxin, BAI Qiwen, ZHANG Junling, LI Jing. Comparison of Efficiency and Safety Between Radiofrequency Ablation and Stereotactic Body Radiotherapy on Primary Small Hepatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2017, 44(12): 831-835. DOI: 10.3971/j.issn.1000-8578.2017.17.0613
    [2]LU Sicong, LU Haiming, LIU Lei, JIANG Shuiming, ZHANG Qishun. Emergency Transcatheter Arterial Embolization Combined with Two-stage Liver Resection for Spontaneous Ruptured Hepatocellular Carcinoma: A Systematic Review[J]. Cancer Research on Prevention and Treatment, 2015, 42(10): 997-1000. DOI: 10.3971/j.issn.1000-8578.2015.10.010
    [3]LU Jingning, YANG Hong, PENG Minhao, XIAO Kaiyin, PENG Tao, PENG Jinbo. Percutaneous Ultrasound-guided Radiofrequency Ablation with Artificial Ascites for Hepatocellular Carcinoma in Hepatic Dome[J]. Cancer Research on Prevention and Treatment, 2015, 42(05): 493-497. DOI: 10.3971/j.issn.1000-8578.2015.05.016
    [4]Shen Xinying, Zhang Yanfang, Kong Jian, Dou Yongchong, Li Yong, Cai Jinzhong, He Fan, Chen Xudong. Therapeutic Value of Radiofrequency Ablation for Treatment of Hepatocellular Carcinoma with Arterioportal Shunts[J]. Cancer Research on Prevention and Treatment, 2012, 39(09): 1120-1124. DOI: 10.3971/j.issn.1000-8578.2012.09.016
    [5]PENG Qi-rong, XIAO Bi, ZHANG Jian, CHENG Tao, CHEN Guo-qiang. Percutaneous Microwave Coagultion Therapy Influence on Hepatofibrosis of Primary Hepatocellular Carcinoma Serum[J]. Cancer Research on Prevention and Treatment, 2011, 38(03): 322-323. DOI: 10.3971/j.issn.1000-8578.2011.03.022
    [6]HUANG Jin-qiu, PENG Min-hao, ZOU Quan-qing, YANG Ding-hua, CHEN Bin, XIAO Kai-yin. Cox Model Analysis of Prognostic Factors after Radical Hepatectomy for Primary Hepatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2009, 36(02): 137-139. DOI: 10.3971/j.issn.1000-8578.2009.02.015
    [8]MA Guoan. RFA Combined with TACE for Primary Hepatic Carcinoma[J]. Cancer Research on Prevention and Treatment, 2006, 33(12): 905-907. DOI: 10.3971/j.issn.1000-8578.3352
    [9]SONG Bin, WANG Wei-xing, ZHANG Wen, et al. Effect of the different combined therapy methods of transcatheter arterial chemoembolization (TACE) and percutaneous radio-frequency ablation(RF) on the elder patients with unresectable hepatocellular carcinoma(HCC)[J]. Cancer Research on Prevention and Treatment, 2003, 30(06): 496-497. DOI: 10.3971/j.issn.1000-8578.2920
    [10]Jiang Xiao-Qing, Yin Guang-fu, Su Ka-qing, Wang Hao. Study on apoptosis of hepatocellular carcinoma cells after intrahepatic arterial angiotensin Ⅱ infusion induced hypertension and chemoembolization (IHCE )[J]. Cancer Research on Prevention and Treatment, 1996, 23(6): 350-351.

Catalog

    Article views PDF downloads Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return