| Citation: |
SHAN Wulin, DENG Fang, ZHANG Xiaolei, ZHANG Jing, HAN Dandan, WAN Lingling, LI Ming. Impact of miRNA-200c on Methotrexate Resistance of Non-small Cell Lung Cancer Cells A549[J]. Cancer Research on Prevention and Treatment, 2016, 43(5): 321-325. DOI: 10.3971/j.issn.1000-8578.2016.05.001
|
To explore the effect of microRNA-200c (miR-200c) on methotrexate (MTX) resistance of non-small cell lung cancer cells A549 (A549/MTX) and elucidate its related mechanism.
Quantitative real-time PCR (qRT-PCR) was used to detect the miR-200c expression in A549 cells, A549/MTX cells transfected with miR-200c mimic (A549/MTX-M) and A549/MTX cells transfected with miR-negative control (A549/MTX-N). MTT assay, Trypan blue staining and flow cytometry analysis were sequentially performed to detect the sensitivity of A549, A549/MTX-M and A549/MTX-N cells to MTX, cell proliferation and apoptosis. qRT-PCR was used to detect the gene expressions of P53 and P21 in these cells.
The miR-200c expression in A549 cells was significantly higher than that in A549/MTX-N cells. The miR-200c expression in A549/MTX-M cells was significantly higher than that in A549/MTX-N cells. Compared with A549/MTX-N cells, the proliferation inhibition and apoptosis of A549/MTX-M cells were increased after treated with MTX in a concentration-dependent manner, with significant difference. Furthermore, the up-regulated P53 and P21 expression were observed in A549/MTX-M cells, with significant difference compared with A549/MTX-N cells(P=0.023, P=0.015).
miR-200c could reduce the resistance of A549/MTX cells to MTX with the possible mechanism of inducing the apoptosis through the P53/P21 pathway.
| [1] |
Maheswaran S, Sequist LV, Nagrath S, et al. Detection of mutations in EGFR in circulating lung-cancer cells[J]. N Engl J Med, 2008, 359(4): 366-77.
|
| [1] |
Maheswaran S, Sequist LV, Nagrath S, et al. Detection of mutations in EGFR in circulating lung-cancer cells[J]. N Engl J Med, 2008, 35 9(4): 366-77.
|
| [2] |
Fiorucci G, Chiantore MV, Mangino G, et al. Cancer Regulator MicroRNA: Potential Relevance in Diagnosis, Prognosis and Treatment of Cancer[J]. Curr Med Chem, 2012, 19(4): 461-74.
|
| [2] |
Fiorucci G, Chiantore MV, Mangino G, et al. Cancer Regulator MicroRNA: Potential Relevance in Diagnosis, Prognosis and Treatment of Cancer[J]. Curr Med Chem, 2012, 19(4): 461-74.
|
| [3] |
Li Y, VandenBoom TG 2nd, Kong D, et al. Up-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cells[J]. Cancer Res, 2009, 69(16): 6704-12.
|
| [3] |
Li Y, VandenBoom TG 2nd, Kong D, et al. Up-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cells[J]. Cancer Res, 2009, 69(16): 6704-12.
|
| [4] |
Ceppi P, Mudduluru G, Kumarswamy R, et al. Loss of miR-200c expression induces an aggressive, invasive, and chemoresistant phenotype in non-small cell lung cancer[J]. Mol Cancer Res, 20 10, 8(9): 1207-16.
|
| [4] |
Ceppi P, Mudduluru G, Kumarswamy R, et al. Loss of miR-200c expression induces an aggressive, invasive, and chemoresistant phenotype in non-small cell lung cancer[J]. Mol Cancer Res, 2010, 8(9): 1207-16.
|
| [5] |
Chen J, Tian W, Cai H, et al. Down-regulation of microRNA-200c is associated with drug resistance in human breast cancer[J]. Med Oncol, 2012, 29(4): 2527-34.
|
| [5] |
Chen J, Tian W, Cai H, et al. Down-regulation of microRNA-200c is associated with drug resistance in human breast cancer[J]. Med Oncol, 2012, 29(4): 2527-34.
|
| [6] |
Ru P, Steele R, Hsueh EC, et al. Anti-miR-203 upregulates SOCS3 expression in breast cancer cells and enhances cisplatin chemosensitivity[J]. Genes Cancer, 2011, 2(7): 720-7.
|
| [6] |
Ru P, Steele R, Hsueh EC, et al. Anti-miR-203 upregulates SOCS3 expression in breast cancer cells and enhances cisplatin chemosensitivity[J]. Genes Cancer, 2011, 2(7): 720-7.
|
| [7] |
Li Z, Hu S, Wang J, et al. MiR-27a modulates MDR1/P-glycoprotein expression by targeting HIPK2 in human ovarian cancer cells[J]. Gynecol Oncol, 2010, 119(1): 125-30.
|
| [7] |
Li Z, Hu S, Wang J, et al. MiR-27a modulates MDR1/P-glycoprotein expression by targeting HIPK2 in human ovarian cancer cells[J]. Gynecol Oncol, 2010, 119(1): 125-30.
|
| [8] |
Chen Y, Zuo J, Liu Y, et al. Reversion of the resistance of gastric cancer SGC7901/DDP cells by miRNA-200c and its related mechanism[J]. Zhong Liu, 2010, 30(8): 646-50. [陈勇, 左静, 刘 颖, 等. miRNA-200c逆转胃癌SGC7901/DDP细胞对顺铂的耐 药性及其相关机制[J]. 肿瘤, 2010, 30(8): 646-50.]
|
| [8] |
陈勇, 左静, 刘颖, 等. miRNA-200c逆转胃癌SGC7901/DDP细胞对顺铂的耐药性及其相关机制[J]. 肿瘤, 2010, 30(8): 646-50.
Chen Y, Zuo J, Liu Y, et al. Reversion of the resistance of gastric cancer SGC7901/DDP cells by miRNA-200c and its related mechanism[J]. Zhong Liu, 2010, 30(8): 646-50.
|
| [9] |
Cochrane DR, Spoelstra NS, Howe EN, et al. MicroRNA-200c mitigates invasiveness and restores sensitivity to microtubule-targeting chemotherapeutic agents[J]. Mol Cancer Ther, 2009, 8(5): 1055-66.
|
| [9] |
Cochrane DR, Spoelstra NS, Howe EN, et al. MicroRNA-200c mitigates invasiveness and restores sensitivity to microtubuletargeting chemotherapeutic agents[J]. Mol Cancer Ther, 2009, 8( 5): 1055-66.
|
| [10] |
Cochrane DR, Howe EN, Spoelstra NS, et al. Loss of miR- 20 0c: a marker of aggressiveness and chemoresistance in female reproductive cancers[J]. J Oncol, 2010, 2010: 821717.
|
| [10] |
Cochrane DR, Howe EN, Spoelstra NS, et al. Loss of miR-200c: a marker of aggressiveness and chemoresistance in female reproductive cancers[J]. J Oncol, 2010, 2010: 821717.
|
| [11] |
Leskelä S, Leandro-García LJ, Mendiola M, et al. The miR-200 family controls β-tubulin Ⅲ expression and is associated with paclitaxel-based treatment response and progression-free survival in ovarian cancer patients[J]. Endocr Relat Cancer, 2010, 18(1): 85-95.
|
| [11] |
Leskelä S, Leandro-García LJ, Mendiola M, et al. The miR-200 family controls β-tubulin Ⅲ expression and is associated with paclitaxel-based treatment response and progression-free survival in ovarian cancer patients[J]. Endocr Relat Cancer, 2010, 18(1): 85-95.
|
| [12] |
Magenta A, Cencioni C, Fasanaro P, et al. miR-200c is upregulated by oxidative stress and induces endothelial cell apoptosis and senescence via ZEB1 inhibition[J]. Cell Death Differ, 2011, 18(10): 1628-39.
|
| [12] |
Magenta A, Cencioni C, Fasanaro P, et al. miR-200c is upregulated by oxidative stress and induces endothelial cell apoptosis and senescence via ZEB1 inhibition[J]. Cell Death Differ, 2011, 18 (10): 1628-39.
|
| [13] |
Mencia N, Selga E, Noé V, et al. Underexpression of miR-224 in methotrexate resistant human colon cancer cells[J]. Biochem Pharmacol, 2011, 82(11): 1572-82.
|
| [13] |
Mencia N, Selga E, Noé V, et al. Underexpression of miR-224 in methotrexate resistant human colon cancer cells[J]. Biochem Pharmacol, 2011, 82(11): 1572-82.
|
| [14] |
Huang WY, Yang PM, Chang YF, et al. Methotrexate induces apoptosis through p53/p21-dependent pathway and increases E-cadherin expression through downregulation of HDAC/EZH2[J]. Biochem Pharmacol, 2011, 81(4): 510-7.
|
| [14] |
Huang WY, Yang PM, Chang YF, et al. Methotrexate induces apoptosis through p53/p21-dependent pathway and increases E-cadherin expression through downregulation of HDAC/ EZH2[J]. Biochem Pharmacol, 2011, 81(4): 510-7.
|
| [15] |
Song B, Wang Y, Kudo K, et al. miR-192 Regulates Dihydrofolate Reductase and Cellular Proliferation Through the p53-miRNA Circuit[J]. Clin Cancer Res, 2008, 14(24): 8080-6.
|
| [15] |
Song B, Wang Y, Kudo K, et al. miR-192 Regulates Dihydrofolate Reductase and Cellular Proliferation Through the p53-miRNA Circuit[J]. Clin Cancer Res, 2008, 14(24): 8080-6.
|
| [16] |
Mishra PJ, Song B, Mishra PJ, et al. MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism[J]. PLoS One, 2009, 4(12): e8445.
|
| [16] |
Mishra PJ, Song B, Mishra PJ, et al. MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism[J]. PLoS One, 2009, 4(12): e8445.
|
| [17] |
Song B, Wang Y, Titmus MA, et al. Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells[J]. Mol Cancer, 2010, 9: 96.
|
| [17] |
Song B, Wang Y, Titmus MA, et al. Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells[J]. Mol Cancer, 2010, 9: 96.
|
This work is licensed under a Creative Commons Attribution 3.0 License.
Copyright © Editorial Department of Cancer Prevention Research 鄂公网安备 42011102005013号 鄂ICP备2022015867号
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: