Abstract: Objective To explore the relationship between vascular endothelial growth factor(VEGF) gene promoter region-460 T/C single nucleotide polymorphisms(SNP) and nasopharyngeal carcinoma(NPC) susceptibility in Guangxi population. Methods We analyzed SNP of VEGF-460 T/C in 240 patients with NPC and 245 healthy controls, using polymerase chain reaction(PCR) sequence specific primers method. The association of the polymorphism with NPC susceptibility was determined by multivariate logistic regression analysis after mixed factors revision. Results Genotypic distributions of VEGF-460 T/C polymorphisms were in Hardy-Weinberg equilibrium. The distribution of VEGF-460 T/T(52.9%) and T/C+C/C(47.1%) differed from those(63.3%,36.7%) in control group(χ2=5.335，P=0.021). Compared with control group(20.6%), the frequency of C allele in NPC group(27.5%) was significantly higher(χ2=6.303，P=0.012). Compared with T allele, the C allele might increase the risk of nasopharyngeal carcinoma (OR=1.458，95%CI:1.115~1.952). Furthermore, compared with genotype T/T, the genotype T/C+C/C containing C allele increased the risk of lymphatic metastasis （OR=2.214，P=0.038）. Conclusion There is significant correlation between VEGF-460 T/C polymorphism and NPC susceptibility.