| Citation: |
ZHANG Lin'gang, ZHANG Tianyu, TAN Ning, YAO Shihong, ZHANG Jianhua. Combined Application of Vitamin C and Arsenic Trioxide in Inhibiting Bladder Cancer Cell Lines T-24 in vitro[J]. Cancer Research on Prevention and Treatment, 2017, 44(2): 103-107. DOI: 10.3971/j.issn.1000-8578.2017.02.005
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To evaluate the effects of vitamin C and arsenic trioxide (As2O3) on bladder cancer T-24 cells and its mechanism.
T-24 cells cultured in vitro were divided into 6 groups randomly, First,the control group was given no especial treatment.Second,the different concentrations of As2O3 group were treated with As2O3 at the concentration of 0.4, 4, 40μg/ml. Third, the vitamin C group was treated with vitamin C of 400μg/ml, the combined group was treated with vitamin C of 400μg/ml and 0.4μg/ml As2O3. The cell proliferation ability was assayed with cell growth curve. The cell cycle and apoptosis ratio of T-24 cells were analyzed with FCM. The expressions of caspase-3 and survivin mRNA of T-24 cells were detected by RT-PCR, while expressions of caspase-3 and survivin proteins were detected by Western blot.
The combined group, vitamin C group (400μg/ml), As2O3 group (4μg/ml) and As2O33 group (40μg/ml) significantly inhibited the growth of T-24 cell lines. The cells major stagnation was stayed in the G0/G1 in the combined group, while apoptosis rate was significantly higher than the other groups. The expression of caspase-3 mRNA and protein were significantly up-regulated in vitamin C group (400μg/ml) and the combined group, while down-regulated the expression of survivin mRNA and protein.
Vitamin C joint As2O3 can inhibit the proliferation of bladder cancer T-24 cells and promote cell apoptosis. The mechanism might be related to elevate the expression of caspase-3 mRNA and protein, reduce the expression of survivin mRNA and protein.
| [1] |
张明, 吴风鹏, 石金苓, 等. 条件复制腺病毒Ad-MK联合丝裂霉素对膀胱癌细胞的作用[J]. 中华实验外科杂志, 2012, 29(8): 1633.
Zhang M, Wu FP, Shi JC, et al. Combined application of replication adenovirus AD-Mk and mitomycin in bladder cancer cell[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2012, 29(8): 1633.
|
| [2] |
张勇, 江文凛, 王平. 保留膀胱切除术联合紫杉醇介入治疗局部浸润性膀胱癌临床疗效[J]. 中国生化药物杂志, 2012, 33(5): 650-1. http://www.cnki.com.cn/Article/CJFDTOTAL-SHYW201205040.htm
Zhang Y, Jiang WB, Wang P. Clinical effect of bladder preserving resection and paclitaxel interventional treatment of invasive bladder cancer[J]. Zhonghua Sheng Hau Yao Wu Za Zhi, 2012, 33(5): 650-1. http://www.cnki.com.cn/Article/CJFDTOTAL-SHYW201205040.htm
|
| [3] |
李海燕, 曹励民, 王娟. 三氧化二砷对人肝癌细胞SMMC-7721增殖、迁移及侵袭能力的影响[J]. 中国老年学杂志, 2013, 33(11): 2572-4. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLXZ201311042.htm
Li HY, Cao LM, Wang J. Inhibition on proliferation, migration and invasion in human hepatocellular carcinoma SMMC-7721 cells by arsenic trioxide[J]. Zhongguo Lao Nian Xue Za Zhi, 2013, 33(11): 2572-4. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLXZ201311042.htm
|
| [4] |
张小坤, 罗建民, 孙杰. 三氧化二砷联合5-杂氮-2′-脱氧胞苷对U937细胞SHP-1、JAK3、TYK2基因表达的影响[J]. 肿瘤防治研究, 2014, 41(7): 728-33. http://www.zlfzyj.com/CN/article/searchArticleResult.do#
Zhang XK, Luo JM, Sun J. Effects of 5-Aza-2′deoxycytidine.Combined with Arsenic Trioxide on SHP-1, JAK3, TYK2 Expression in U937 Cells[J]. Zhong Liu Fang Zhi Yan Jiu, 2014, 41(7): 728-33. http://www.zlfzyj.com/CN/article/searchArticleResult.do#
|
| [5] |
Blasiak J, Kowalik J. Protective action of vitamin C against DNA damage induced by selenium-cisplatin conjugate[J]. Acta biochim Pol, 2001, 48(1): 233-40.
|
| [6] |
Grad JM, Bahlis NJ, Reis I, et al. Ascorbic acid enhances arsenic trioxide-induced cytotoxicity in multiple myeloma cells[J]. Blood, 2001, 98(3): 805-13. doi: 10.1182/blood.V98.3.805
|
| [7] |
Chen G, He Y, Wu X, et al. In vitro and in vivo studies of pirarubicin-loaded SWNT for the treatment of bladder cancer[J]．Braz J Med Biol Res, 2012, 45(8): 77l-6．
|
| [8] |
Zhang X, Jia S, Yang S, et al. Arsenic trioxide induces G2/M arrest in hepatocelhlar carcinoma cells by increasing the tumor suppressor PT'EN expression[J]. J Cell Biochem, 2012, 113(11): 3528-35． doi: 10.1002/jcb.24230
|
| [9] |
袁坦, 姜国忠, 李宏云. 三氧化二砷对肺癌细胞NCI-H520的影响[J]. 中华实验外科杂志, 2014, 31(6): 1292-3.
Yuan T, Jiang GZ, Li HY. Influences of arsenic trioxide on lung cancer cell line NCI-H520[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2014, 31(6): 1292-3.
|
| [10] |
Bürzle M, Suzuki Y, Ackermann D, et al. The sodium dependent ascorbic acid transporter family SLC23[J]. Mol Aspects Med, 2013, 34(2-3): 436-54． doi: 10.1016/j.mam.2012.12.002
|
| [11] |
Yu R, Schellhorn HE. Recent applications of engineered animal antioxidant deficiency models in human nutrition and chronic disease[J]. J Nutr, 2013, 143(1): 1-11. doi: 10.3945/jn.112.168690
|
| [12] |
曾锦荣, 蒋碧佳, 王绩英. 三氧化二砷联合维生素C对耐药性肺腺癌裸鼠移植瘤caspase-3、survivin活性的影响[J].中国老年学杂志, 2011, 31(24): 4827-31. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLXZ201124042.htm
Zeng JR, Jiang BJ,Wang JY. Arsenic Trioxide(AS2O3) combined with vitamin C(Vit C) affect the activity of effect of arsenic trioxide(AS2O3) combined with vitamin C on caspase-3 and surviving in the subcutaneously transplanted tumor of drug resistant human lung carcinoma in nude mice[J]. Zhongguo Lao Nian Xue Za Zhi, 2011, 31(24): 4827-31. http://www.cnki.com.cn/Article/CJFDTOTAL-ZLXZ201124042.htm
|
| [13] |
Haga N, Fujita N, Tsuruo T. Involvement of mitochondrial aggregation in arsenic trioxide(AS2O3)-induced apoptosis in human glioblastoma cells[J]. Cancer Sci, 2005, 96(11): 825-33. doi: 10.1111/cas.2005.96.issue-11
|
| [14] |
Li X, Guan X, Li F, et al. Chemosensitivity enhancement toward arsenic trioxide by inhibition of histone deacetylase in NB4 cell line[J]. J Int Med Res, 2016, 44(4): 882-92. doi: 10.1177/0300060516646238
|
| [15] |
Beauchamp EM, Kosciuczuk EM, Serrano R, et al. Direct Binding of Arsenic Trioxide to AMPK and Generation of Inhibitory Effects on Acute Myeloid Leukemia Precursors[J]. Mol Cancer Ther, 2015, 14(1): 202-12. doi: 10.1158/1535-7163.MCT-14-0665-T
|
| [16] |
杨建萍, 李晟磊, 赵志华, 等. 雷帕霉素及mTOR siRNA对宫颈癌HeLa细胞mTOR和4EBP1表达的影响[J]. 肿瘤防治研究, 2014, 41(1): 40-5. http://www.zlfzyj.com/CN/article/searchArticleResult.do#
Yang JP, Li SL, Zhao ZH, et al. Effects of mTOR siRNA and Rapamycin on mTOR and 4EBP1 Expressions in Human HeLa Cells[J]. Zhong Liu Fang Zhi Yan Jiu, 2014, 41(1): 40-5. http://www.zlfzyj.com/CN/article/searchArticleResult.do#
|
| [17] |
梁小燕, 王丕龙, 陶小红. LKB1通过AMPK信号通路抑制Hela细胞增殖的机制研究[J]. 重庆医科大学学报, 2013, 38(1): 45-7. http://www.cnki.com.cn/Article/CJFDTOTAL-ZQYK201301012.htm
Liang XY, Wang PL, Tao XH. Inhibition of Hela cell proliferation by LKB1 through AMPK signaling pathway[J]. Chongqing Yi Ke Da Xue Xue Bao, 2013, 38(1): 45-7. http://www.cnki.com.cn/Article/CJFDTOTAL-ZQYK201301012.htm
|
| [18] |
高飞, 易静, 史桂英, 等. 维生素C增强氧化砷联用二甲萘醌诱导的U937细胞凋亡及其机制[J]. 中华血液学杂志, 2002, 23(1): 9-11. http://www.cnki.com.cn/Article/CJFDTOTAL-ZHXY200201001.htm
Gao F, Yi J, Shi GY, et al. Ascorbic acid enhances the apoptosis of U937 cells induced by arsenic trioxide in combination with DMNQ and its mechanism[J]. Zhonghua Xue Ye Xue Za Zhi, 2002, 23(1): 9-11. http://www.cnki.com.cn/Article/CJFDTOTAL-ZHXY200201001.htm
|
| [19] |
Fritz H, Flower G, Weeks L, et al. Intravenous Vitamin C and Cancer: A Systematic Review[J]. Integr Cancer Ther, 2014, 13(4): 280-300. doi: 10.1177/1534735414534463
|
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