Objective To explore the molecular mechanism of Jinlong capsule reversing paclitaxel resistance of paclitaxel-resistant lung adenocarcinoma cell line A549 by system biology technology.
Methods Human Genome U133 Plus 2.0 genechip was used to detect the genes of samples which included A549 paclitaxel-resistant lung adenocarcinoma cell line A549 with Jinlong capsule’s intervention and control group. Then differentially expressed genes were identified based on fold change between the two groups. The topologically significant genes were obtained by one-step overconnectivity test and muti-steps hidden nodes algorithm; the biological function was analyzed by enrichment analysis; the causal network was constructed on Meta Core platform.
Results Compared with the control group, 203 differentially expressed genes had been identified in Jinlong capsule-treated group(ratio>2). The connection analysis identified 416 topologically significant genes. The causal network model revealed that the target of Jinlong capsule might be associated with down-regulation of the cell cycle genes and up-regulation of immune response.
Conclusion Jinlong capsule could reverse paclitaxel resistance in paclitaxel-resistant lung adenocarcinoma cell line by A549 regulating cell cycle and immune response.
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