Galangin Induces Apoptosis on Lung Cancer A549 Cells

Objective To investigate apoptosis induced by Galangin in lung cancer A549 cells. Methods Cell inhibition ratio was assayed by MTT method. Morphologic assessment of apoptosis was performed with a fluorescence microscope,and cell cycle and mitochondrial membrane potential were analyzed by flow cytometry.The expression levels of proteins were measured by Western blot. ResultsA549 cells were inhibited by Galangin in a dose-dependent manner.IC50 of Galangin was 30.15 mg/L. The apoptosis appeared in 24 hours after treated by Galangin. Morphologic changes including nuclear chromatin condensation fluorescence strength was observed through fluorescence microscope.Caspases were activated.The expression of Bcl-2 and Bcl-XL decreased，while the expression of p53,Bax and Bid increased. ConclusionGalangin could induce A549 cell apoptosis through mitochondria pathway.