Effect of Leukemia Inhibitory Factor on Proliferation and Invasion of Non-small Cell Lung Cancer Cells and Related Mechanism

LI Hao; CUI Yong

doi:10.3971/j.issn.1000-8578.2015.06.004

Cancer Research on Prevention and Treatment > 2015 > 42(06): 553-555. > DOI: 10.3971/j.issn.1000-8578.2015.06.004

LI Hao, CUI Yong. Effect of Leukemia Inhibitory Factor on Proliferation and Invasion of Non-small Cell Lung Cancer Cells and Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2015, 42(06): 553-555. DOI: 10.3971/j.issn.1000-8578.2015.06.004

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Effect of Leukemia Inhibitory Factor on Proliferation and Invasion of Non-small Cell Lung Cancer Cells and Related Mechanism

LI Hao,
CUI Yong

Department of Thoracic Surgery, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China

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Received Date: May 27, 2014
Revised Date: September 16, 2014

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Abstract

Abstract

Objective To explore the influence of leukemia inhibitory factor(LIF) on the proliferation and invasion of non-small cell lung cancer(NSCLC) cells. Methods The expression of LIF in human NSCLC tissues and adjacent tissues were detected by fluorescent quantitative PCR and Western blot. After NSCLC cell lines A549 and Z793 were treated with recombinant LIF, we detected cell proliferation by MTT assay, cell invasion by Transwell assay, and ATK/mTOR pathway by Western blot. Results LIF expression was higher in lung cancer tissues than that in the matched adjacent tissues(P=0.0078). After A549 and Z793 cell lines were treated with recombinant LIF, the tumor cells showed a significantly higher proliferation rate and invasiveness in vitro than those in control group(P<0.01); moreover, the phosphorylated AKT level was significantly elevated and the expression of mammalian target of rapamycin(mTOR), the AKT downstream targets, was obviously up-regulated. Conclusion LIF expression is significantly increased in NSCLC cells. In addition, the ectopic expression of LIF promotes cell proliferation and invasion via activating AKT/mTOR pathway.
- Leukemia inhibitory factor(LIF),
- Non-small cell lung cancer(NSCLC),
- Proliferation,
- Invasion,
- AKT

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References

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