Correlation Analysis of miR-20a Expression and Relapse in Formalin-fixed Paraffinembedded Tissue of Urothelial Carcinoma of Bladder

REN Hailin; LI Xiaona; CHEN Guojun; SUO Jiming; SUN Yan; WANG Jian

doi:10.3971/j.issn.1000-8578.2014.06.025

Cancer Research on Prevention and Treatment > 2014 > 41(06): 622-625. > DOI: 10.3971/j.issn.1000-8578.2014.06.025

REN Hailin, LI Xiaona, CHEN Guojun, SUO Jiming, SUN Yan, WANG Jian. Correlation Analysis of miR-20a Expression and Relapse in Formalin-fixed Paraffinembedded Tissue of Urothelial Carcinoma of Bladder[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 622-625. DOI: 10.3971/j.issn.1000-8578.2014.06.025

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Correlation Analysis of miR-20a Expression and Relapse in Formalin-fixed Paraffinembedded Tissue of Urothelial Carcinoma of Bladder

1. Department of Urology, Affi liated Hospital of Qinghai University, Xining 810001, China;2. Department of Clinical Laboratory, The People's Hospital of Qinghai;3. Tianjin Institute of Urinary Surgery

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Received Date: April 01, 2013
Revised Date: July 16, 2013

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Abstract

Abstract

Objective To investigate the feasibility of extracting microRNA from formalin-fixed paraffinembedded(FFPE) tissue of bladder urothelial carcinoma(UC), and analyze the correlation among miR-20a expression, clinicopathologic feature and the relapse. Methods The expression levels of miR-20a in freshly frozen(FF) and FFPE tissues from 50 cases of bladder UC were detected by quantitative real-time PCR. The expression levels of miR-20a in FFPE tissue from 181 cases of bladder UC and its correlation with the relapse in different years were analyzed. Results The expression of miR-20a were closely correlated in FFPE and FF tissue(r=0.792,P＜0.001). The expression levels of miR-20a in FFPE tissue in different years were stable (P＞0.05). The expression of miR-20a was closely correlated with clinicopathological features. The relapse rate of patients with high expression of miR-20a was signifi cantly higher(P＜0.05). Conclusion The expression levels of miR-20a in FF and FFPE tissue of bladder UC were consistency and reliable, hence, it is feasible to extract and study the microRNA in FFPE tissues. The role of miR-20a was correlated with its expression level, and a high expression level is the independent factor of postoperative relapse.
- Bladder neoplasms,
- miR-20a,
- Formalin-fi xed paraffi n-embedded tissue,
- Relapse

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