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Wang Cheng, Sui Chunhong, Liu Wei, Xu Ye, Yu Yang, Liu Shibing, Lv Shijie, Pan Wengan. Effect of Microwave Radiation on Proliferation and Differentiation of Human Neuroblastoms SH-SY5Y Cells[J]. Cancer Research on Prevention and Treatment, 2012, 39(06): 671-675. DOI: 10.3971/j.issn.1000-8578.2012.06.014
Citation: Wang Cheng, Sui Chunhong, Liu Wei, Xu Ye, Yu Yang, Liu Shibing, Lv Shijie, Pan Wengan. Effect of Microwave Radiation on Proliferation and Differentiation of Human Neuroblastoms SH-SY5Y Cells[J]. Cancer Research on Prevention and Treatment, 2012, 39(06): 671-675. DOI: 10.3971/j.issn.1000-8578.2012.06.014

Effect of Microwave Radiation on Proliferation and Differentiation of Human Neuroblastoms SH-SY5Y Cells

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  • Received Date: August 07, 2011
  • Revised Date: October 17, 2011
  • Objective The influence of microwave radiation in the proliferation and differentiation of human neuroblastoma SH-SY5Y cells were investigated. Methods The SH-SY5Y cells were radiated for 5 minutes by 0(the control group),10,30,50 mW/cm2 of microwave .The prolife ration rates were counted by trypan blue staining.After the cells were induced by 20 μmol/L all-trans retinoic acid(ATRA) in the dark 72 h,the differentiation rates were calculated under the light microscope.The cell cycles were analyzed by flow cytometry(FCM).The expressions of NF-H proteins were detected by Western blotting. Results The proliferation rates of SH-SY5Y cells were reduced gradually with the increases of microwave radiation intensity.Compared with control group,the proliferation of SH-SY5Y cells were significantly inhibited in 10,30,50 mW/cm2 radiation group respectively at 72,48 and 12 h after microwave radiation.After the cells were induced 72 h by 20 μmol/L ATRA,the numbers of differentiated cells of 30,50 mW/cm2 radiation groups were less than control group,and the cell axons of various radiation groups were shorter than control group.With the increase of radiation intensity,microwave increased the proportion of SH-SY5Y cells in G0/G1 phase,and decreased the proportion in the S phase.The proportion of SH-SY5Y cells in G2+M phase cells was unchanged.After microwave radiation,the expressions of NF-H protein were not changed,but the expressions of phosphorylated NF-H proteins were decreased gradually. Conclusion The proliferation and differentiation of SH-SY5Y cells can be inhibited by microwave radiation in an obvious dose-dependent manner.Cell cycle progression was blocked in the G0/G1 phase.Microwave had no effect on the expression of NF-H in SH-SY5Y cells,but could inhibit NF-H phosphorylation significantly.
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