Effect of Gemzar Combined with Electron Ray on Tumor Apoptosis in Nude Mice Xenograft of Human Pancreatic Carcinoma

ObjectiveTo investigate the effect of gemzar combined with electron ray on tumor apoptosis in nude mice xenograft of human pancreatic carcinoma. Methods36 xenograft models of human pancreatic carcinoma were established.They were randomly divided into control group(A) radiation group (B) Gemzar 25 mg/kg group (C) Gemzar 50mg/kg group (D) combined treated group1 (E) and combined treated group2(F). Were injected with normal saline B treated with 6MeV electron ray on the tumor; C and D were injected with Gemzar 25 mg/kg and 50mg/kg respectively and E & F were injected with Gemzar 25 mg/kg and 50mg/kg respectively before treated with additional 6Mev electron ray on the tumor. The method of immunohistochemistry was used to estimate the expression of apoptosis inhibitory protein Bcl-2 and apoptosis associated protein Bax，while the apoptosis of tumor cell was detected by TUNEL to determine apoptotic index(AI). Results Compared with the control group the apoptosis of tumor cell in treated groups was notably raised and the statistical differences of the apoptotic index(AI) among treated group were significant（P＜0.05）.Immunochemical showed that as compared with the control group,expressions of Bcl-2 was down-regulated while Bax was up-regulated in all the treated groups to some extent（both P＜0.05）.Also significant difference was also shown in comparison of the two indices between the combined treated group and group B to D（P＜0.05）. Conclusion Gemzar combined with electron ray effectively induces apoptosis of tumor cells in the xenografts.