Advanced Search
Radiation-Induced Mitotic Apoptosis in Human lung Adenocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 1999, 26(6): 465-466.
Citation: Radiation-Induced Mitotic Apoptosis in Human lung Adenocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 1999, 26(6): 465-466.

Radiation-Induced Mitotic Apoptosis in Human lung Adenocarcinoma Cells

More Information
  • Objective To investigat the mode of cell death after irradiation in one human lung adenocarcinoma cell line and a human normal lung fibroblast cell line. Methods The morphology of dead cells and dou-ble strands break in apoptotic cells were observed by HE stain and comet assay.The variety of cell cycle and emerging time of apoptosis after irradiation were examined by flow cytometry analysis. Results Apoptotic cells emerged in the lung adenocarcinoma cells after irradiation and appeared in G1 arrest subsequent to overcoming G2 arrest after irradiation.Only was necrosis observed in the fibroblast cells. Conclusion Apoptosis is one of the ultimate forms of cell death via mitotic failure caused by irradiation in lung adenocarcinoma cells.The advent of mitotic apoptosis depended on the radiation doses and incubation time. The fact that mitotic mecrosis might be the major cause of fibroblast death demonstrated that mitotic death modes were complex and various in different lines.
  • 1 Shinohara K and Nakano H, Interphase death and reproductive death in X-irradiation Molts cells. Radial ftes, 1993, 135, 197一205
    2 Olive P L, Banath J P and Durand R E, Heterogeneity in radiationinduced DNA damage and repair in tumor and nomjal cells rtreasured using the Comet assay. Radial Res, 1990. 122, 86一94
    3 左连富主编.流式细胞术样品制备技术.北京:华夏出版社,1991,49一50
    4 Yanagihara K, Nii M, Numoto M, et al. Radiation-induced apol folic cell death in human gastric epithelial tumour cell; correlation between mitotic death and apoptosis. Int Radial Biol, 1995, 67,677一685
    5 Waters R L. Radiation-induced apoptosis in a marine T-cell hybridoma. Cancer Res, 1992, 52, 883一890
    6 Radford 1 R, Muphy T K, Radley J M, et al. Radiation response of mouse lymphoid and myeloid cell lines part JI..4 Apoptotic death is shown by all lines examined. Int J Radial Biol 1994, 65, 217一227
    7 Tauchi H and Sawada S, Analysis of mitotic cell death caused radiation in mouse leukemia 1789 cells: apoptosis is the ultimate form of cell death following mitotic failure. Int J Radial Bid, 1994,65, 449一455
    8 Afanasyev V N, KoroeeBA, Mantsygin PA, et al. Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death. FEBS Letters, 1986, 194, 347一350
    9 Miura M, Zhu-H, Rotello R, et al. Induction of apoptosis in fidro blasts by ICE a mammalian homolog of the C. elegans cell death gene ced-3. Cell, 1993, 75, 653一660

Catalog

    Article views (1015) PDF downloads (305) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return