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磁性纳米颗粒介导基因治疗乳腺癌实验研究

韦卫中, 吴 华, 李 芳

韦卫中, 吴 华, 李 芳. 磁性纳米颗粒介导基因治疗乳腺癌实验研究[J]. 肿瘤防治研究, 2005, 32(08): 473-475. DOI: 10.3971/j.issn.1000-8578.3294
引用本文: 韦卫中, 吴 华, 李 芳. 磁性纳米颗粒介导基因治疗乳腺癌实验研究[J]. 肿瘤防治研究, 2005, 32(08): 473-475. DOI: 10.3971/j.issn.1000-8578.3294
WEI Wei-zhong, WU Hua, LI Fang. Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy —A Study in Vitro on Breast Cancer[J]. Cancer Research on Prevention and Treatment, 2005, 32(08): 473-475. DOI: 10.3971/j.issn.1000-8578.3294
Citation: WEI Wei-zhong, WU Hua, LI Fang. Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy —A Study in Vitro on Breast Cancer[J]. Cancer Research on Prevention and Treatment, 2005, 32(08): 473-475. DOI: 10.3971/j.issn.1000-8578.3294

磁性纳米颗粒介导基因治疗乳腺癌实验研究

详细信息
    通讯作者:

    韦卫中

  • 中图分类号: R737. 9

Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy —A Study in Vitro on Breast Cancer

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    Corresponding author:

    WEI Wei-zhong

  • 摘要: 目的 探讨新型基因载体PEI包裹的磁性纳米颗粒polyMAG-1000介导TRAIL基因治疗乳腺癌的可行性,观察TRAIL基因转染乳腺癌细胞后的治疗作用。方法 以polyMAG-1000为基因载体,联结TRAIL质粒DNA后转染人乳腺癌细胞株MCF-7细胞,用Tunel法检测细胞的凋亡,用流式细胞仪检测细胞的凋亡率,以空载体作为阴性对照;脂质体转染TRAIL基因作阳性对照。结果 Tunel法可见MCF-7细胞经polyMAG1000和脂质体转染TRAIL基因后均可见发生凋亡的细胞,流式细胞仪检测polyMAG-1000转染的细胞凋亡率为25.11%±2.85%,脂质体转染的细胞凋亡率为18.31%±2.44%(P〈0.05)。结论 TRAlL对乳腺癌细胞MCF-7具有凋亡效应,PEI包裹的磁性纳米颗粒介导的TRAIL基因治疗在肿瘤的基因治疗中具有应用前景。

     

    Abstract: Objective  To study the feasibility of using TRAIL gene to treat breast cancer mediated by PEI coated magnetic iron oxide nanoparticles (polyMA G-1000) . The therapeutic efficacy was also be evaluated. Methods  PEI coated magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMA G-1000 without TRAIL gene was t ransfected into the tumor cells as negative cont rol and liposome mediated TRAIL gene t ransfection was taken as positive cont rol. The apoptosis of cells were detected by TUNEL method. The apoptosis ratio of tumor cells were measured with flow cytometry ( FCM) . Results  The apoptosis of cells was demonst rated after transfecting TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMA G-1000 as gene carrier were 25. 11 % ±2. 85 %, whereas it was 5. 06 % ±1. 05 % in the cont rol group with polyMA G-1000 ( P < 0. 01) . The lower apoptosis ratio of 18. 31 % ±2. 44 % was showed in the group with liposome as gene carrier ( P < 0. 05, compared with the group with polyMA G-1000 as gene carrier) . Conclusion  TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The PEI coated magnetic iron oxide nanoparticles may be a potential gene carrier with high transfection efficacy in cancer gene therapy.

     

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出版历程
  • 收稿日期:  2004-10-24
  • 修回日期:  2005-01-25
  • 刊出日期:  2005-08-04

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