Abstract: Objective To explore the correlation of codon194 and codon399 single nucleotide polymorphisms (SNP) of DNA repair gene X-ray repair cross-complementing gene 1(XRCC1) with the sensitivity to platinum chemotherapy in nasopharyngeal carcinoma(NPC)patients. Methods The biopsy specimens were obtained from NPC patients diagnosed by nasopharyngeal endoscopy in The Fourth Affiliated Hospital of Guangxi Medical University from September 1 2012 to December 31 2013. XRCC1 SNP at codon194 and codon399 in NPC patients were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). After 2 periods of PF(cisplatin+5-fluorouracil) induction chemotherapy, MRI was taken to assess the efficacy according to RECIST 1.1 standard, evaluate the relationship between SNP and the sensitivity of chemotherapy in nasopharyngeal cancer patients. Results The patients with XRCC1 codon399 Gln/Gln polymorphisms were 3.500 times as sensitive to the chemotherapy as the patients with Arg/Arg genotype(P<0.05). The patients with XRCC1 codon399 Gln/Gln polymorphisms were 3.274 times as sensitive to the chemotherapy as the patients with (Arg/Arg+Arg/Gln) genotype(P<0.05). The sensitivity to chemotherapy wasn't significant different among the patients with XRCC1 codon194 Arg/Arg, Arg/Trp,Trp/Trp polymorphisms(P>0.05). Conclusion XRCC1 codon399 SNP might be a useful predictive marker in NPC patients treated with cisplatin-based chemotherapy.