Abstract: Objective To observe the curative effect of calf spleen extractive injection combined with docetaxel plus capecitabine chemotherapy on advanced breast cancer patients, adverse reaction and its influence on the immune function. Methods In the trial,106 patients pathologically diagnosed with advanced breast cancer were randomly and equally divided into two groups. The treatment group received docetaxel combined with capecitabine chemotherapy plus compound calf spleen extractive injection; the control group received docetaxel combined with capecitabine chemotherapy alone. Each patient received at least 2 cycles of treatment. Results In the treatment group, the decrease of clinical benefit rate, peripheral white blood cell, haemoglobin and platelet were less than that in the control group. The blood levels of CD3+, CD4+, CD4+/CD8+, NK cell activity in the treatment group after treatment were significantly higher than those before treatment and those in the control group(P＜0. 05; P＜0.01). While in the control group,the blood levels of CD3+, CD4+, CD4+/CD8+, NK cell activity after treatment were significantly lower than those before treatment(P＜0.05). Conclusion The calf spleen extractive injection could attenuate the toxicity of chemical drugs, reduce the myelosuppression, and improve the immunity and QOL of advanced breast cancer patients.