Abstract: Objective To investigate the effi cacy and safety of rendezvous chemotherapy combined with threedimensional conformal radiotherapy in the treatment for post-operative malignant glioma. Methods A total of 99 patients with full resection of malignant glioma by microsurgery and confi rmed by histopathology were collected. 21 patients were performed three-dimensional conformal radiotherapy alone (RT group), 24 patients received nimustine interstitial chemotherapy combined with RT (ACNU +RT group), 23 patients were treated with temozolomide chemotherapy concurrent combined with RT (TMZ+RT group), and 31 patients received nimustine interstitial chemotherapy rendezvous with temozolomide chemotherapy concurrent combined with RT (ACNU+TMZ+RT group). All patients were followed up for observation of median survival time, 1-, 2-, 3-year survival rates and safety. Results The survival rates of 1-, 2- and 3-year of ACNU+TMZ+RT group were 80.6%(25/31), 48.4%(15/31) and 25.8%(8/31), and the median survival time was 29.0（39.8-18.6）months. The survival time of 1-, 2- and 3-year in ACNU +TMZ+RT group were signifi cantly longer than RT, ACNU +RT and TMZ+RT group（χ_L²=21.045, 22.385, P=0.043 and 0.045）. The KPS≥60 rates of 1-, 2-and 3-year in ACNU+TMZ+RT group were 77.4%(24/31), 48.4%(15 /31), 22.8%(8/31),respectively. The life quality of 1-, 2-year in ACNU +TMZ+RT group were signifi cantly superior to RT, ACNU +RT and TMZ+RT group (χ²=8.199,P=0.042), (χ²=7.864,P=0.049). Conclusion With full resection of malignant glioma by microsurgery, rendezvous chemotherapy combined with three-dimensional conformal radiotherapy in the treatment for post-operative malignant glioma is the optimum comprehensive treatment plan, which could signifi cantly extend patient's survival time and improve the life quality.