Abstract: Objective To investigate whether Graphene Oxide (GO) absorbed chemotherapeutic drug and immune stimulators are bioactive in vitro. Methods Graphene Oxide was used to absorb 5-Fu, IFNγ and anti-IL10R antibodies respectively. The biological characteristics of absorbed 5-Fu, IFNγ and anti-IL10R antibodies were examined by microscope, ELISA and Western blot, etc. in vitro. Results GO itself does not kill tumor cells, and does not promote CD11c+ dendritic cells upgrade CD86 expression on their cell membrane. GO absorbed 5-Fu is able to kill tumor cells in vitro, and the killing of tumor cells is dose dependent. GO absorbed 5-FU kills tumor cells more effi ciently than free 5-Fu. GO absorbed IFNγ and anti-IL10R antibodies are able to enhance IL12 secretion by CD11c+ dendritic cells stimulated by LPS. Conclusion Graphene oxide absorbed chemotherapeutic drug and immune stimulators are bioactive in vitro and may provide a new way for cancer therapy.