Abstract: Objective To detect the anti-tumor effect of polypeptide vaccine-FAPτ ?from fibroblast activation protein α. Methods The antigen FAPτ?was mixed uniformly with Freund's incomplete adjuvant and then immunized in multiple locations on the back of mice on day 1, 14 and 21. Tumor cells were implanted into the mice on day 7. Curves of tumor volume change and mice survival were recorded. Murine serum and splenic cells were collected on day 22 after the implantation. Antibody titer of FAPτ ?was detected by ELISA. IFN-?γ expression in CD8⁺T cells was detected by flow cytometer. Results The tumor growth rate was reduced while the survival rate was increased in mice immunized with FAPτ ?vaccine compared that in with PBS control group. Similarly, high level of antibody titer and IFN-γ expression in CD8⁺T cells were detected in mice immunized with FAPτ. Conclusion By activating humoral immune response and CD8⁺T cell immune response effectively, FAPτ?has strong anti-tumor activity.