The effect of quercetin on RNA polymerases and cell cycle in human leukemia HL-60 cell
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摘要: 本文旨在探讨槲皮素对人早幼白血病细胞(HL-60)RNA聚合酶活性和细胞周期的影响及其相互关系.分别用4、20和100μmol/L槲皮素处理培养的HL-60X细胞2天或用20μmol/L槲皮素处理0-5天.应用流式细胞仪进行细胞周期分析,并通过体外核转录实验测定总RNA聚合酶活性和RNA聚合酶Ⅱ活性.结果表明,槲皮素处理HL-60细胞后,体外核转录活性降低,总RNA聚合酶活性尤其是RNA聚合酶Ⅱ活性受抑制,并呈时间和剂量依赖关系;细胞周期中G1期细胞增加,并与作用时间和剂量呈依赖关系,提示槲皮素阻断HL-60细胞周期于G1期;结果分析还表明,槲皮素处理的HL-60细胞,其总RNA聚合酶活性尤其是RNA聚合酶Ⅱ活性受抑制程度与G1期细胞所占百分比增加程度呈明显的相关性.提示槲皮素抑制HL-60细胞体外核转录活性尤其抑制RNA聚合酶Ⅱ活性,可能是槲皮素阻断HL-60细胞周期于G1期的影响因素之一.Abstract: To study foe effect and the relationship of queroetin on RNA polymerases and cell cycle in Human leukemia HL-60 cells.Treating of HL-60 cells with 4,20 and 100μmol/L quercetin for 2 days,or wilh 20μmol/L for 1-5 days,the cell cycle were analyzed with flow cytometer.And total RNA polymerases activity and RNA polymerase Ⅱactivity were measured in isolated HL-60 nuclei by nuclear transcription in vitro.After treatment of HL-60 cells with quercetin,total RNA polymerase activity,especially RAN polymerase Ⅱactivity showed inhibitory effects in time-and dose-dependent manner.The cells at G1,phase increased at the same way.This phenomena revealed that quercetin could arrest cell cycle progression of HL-60 at G1phase.These observation also suggest that quercetin treatment of HL-60 cells gave the inhibitory effects of total RNA polylnerases activity and especially RNA polymerase Ⅱ activity,is related to the cell increase at G1phase.It may be one of reason that queroetin could block in G1 phase of HL-60 cell cycle because of its inhibitory effects on nuclear transcripti-on in vtro,especially on RNA polymelase Ⅱ.
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Key words:
- Quercetin /
- Leukemia /
- Promyelocytic /
- Cell Cycle /
- RNA Polymerases
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