建立裸小鼠人胃癌原位移植模型的一种新方法
Establishment of an model of human gastic cancer in nude mice via orthotopic transplantation
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摘要: 建立人胃癌裸小鼠原位移植模型, 并对不同方法进行比较。 方法:BALB/Cnu/un裸小鼠18只, 随机分为三组, 胃囊法组通过手术将SGC－7901人胃癌组织块移植到裸小鼠胃壁缝制的粘膜小囊内; 缝挂法组以缝挂方法作对比; 第三组为对照组。 待荷瘤鼠濒临死亡时处死进行解剖检查。 结果:胃囊法组及缝挂法组原位成瘤率及局部浸润发生率均为100％，胃囊法组肝脏转移率为66.7％(4/6), 显著P<0.01高于挂法组(33.3％), 且幽门梗阻及癌性腹水发生率亦明显提高(分别为83.33％ vs 16．6％P<0.01; 66.7％vs16.6％, P<0.001)。 结论:胃囊法建立的模型能更好地再现人胃癌的生长特性。Abstract: Objective: to establish an orthotopic transplant model of human stomach cancer and compare two different approaches. Methap: Eighteen BAL/C-nu/nu nude nudce were randomly devided into three groups: for the first group, SGC-7901 human gastric tu-mor tissue pieces were transpianted into a small "pouch" in stomach wall; for the secondgroup,tumor tissue pieces were fixed in the stomach wall directly; and the third groupwas the control. All the tunlor - bearing mice underwent autopsy, Results: Both ap-proaches yielded 100% tunror take rate, 100 % local invasiveness however, liver metasta-sis were higher in the first group than the second one (66.7% vs 33.3%, P<0.00l )and so were pylorochesis and ascites(83.33% vs 16.6%, P<0.00l; 66.7% vs 16.6%, P<0.001, reSPectively). Conclusion: the medel grew more "patient-like" when tunortissue pieces were implanted into the small artificial pouch.
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Key words:
- Gastric cancer /
- Nude mice /
- Disease model /
- Orthotopic implantation
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[1] Liotta, LA, Steeg PS, Stetler-Steveson WG, Cancer metastasis and angiogenesis:an imbalance of positive and negative regulation. Cell, 1991, 64:327. [2] Furukawa T, Fu, X, Kuboba T, et al. Nude mouse metastatic model of human stomach cancer constructed using orthotopic implantanion of histologic intact tissue. Cancer Res, 1993, 53:1204. [3] 汪芳裕, 李绍白, 林菊生, 等. NK活性与裸鼠体内移植肿瘤生长的关系. 中国免疫学杂志, 1995, 11(增刊):492. [4] Giavazzi R. Campblell DE, Jessup JM, et al. Metastatic be-havior of tumor cells isolated from primary and metastatic human colorectal carcinoma implanted into different sites in nude mice. Cancer Res, 1986, 46:1928. [5] Stephenson RA, Dinney CPN, Gohji NG, et al. Metastatic model for human prostate cancer using orthotopic implan-tation in nude mice. J natl Cancer Inst, 1992, 84:951. [6] Fidler IJ. Critical factors in biology of human cancer metas-sis:twenty-eighth G, H, A, Clowes memorial award lec-ture. Cancer Res, 1990, 50:6130. [7] Morikawa K, Walker SM, Nakajima M, et al. Influence of organ environment on the growth selection and metastasis of human colon carcinoma cells in nude mice, Cancer Res, 1988, 48:6863. [8] 孙肪宪, 汤钊猷, 刘康达, 等. 裸鼠人肝癌原位移植模型的生长特性及转移潜能. 中华医学杂志, 1995, 75:673.
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