Abstract: Objective 　To study the correlation of bcl-2 gene expression level with the prognosis in human glioma. Methods 　The expression of bcl-2 protein and bcl-2 mRNA were determined by immunohistochemical staining method and in situ hybridization in 61 samples of human glioma tissues, as well as 20 normal brain tissues. Results 　The positive rates of bcl22 protein (90. 2 %) and bcl-2 mRNA (95. 1 %) in glioma group was significantly higher than those in human normal brain tissues ( P < 0. 01), and a significant correlation was found between bcl22 protein and bcl-2 mRNA expression level, between bcl-2 expression level and pathological grading ( P < 0. 01) . Conclusion 　High bcl-2 expression was prevalent in malignant glioma tissure, and the bcl-2 expression level was a close correlation with the clinical prognosis.