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免疫组织化学法检测肺癌患者EGFR敏感突变的临床研究

杨振华, 叶亮, 方苏榕, 谷伟

杨振华, 叶亮, 方苏榕, 谷伟. 免疫组织化学法检测肺癌患者EGFR敏感突变的临床研究[J]. 肿瘤防治研究, 2016, 43(4): 272-276. DOI: 10.3971/j.issn.1000-8578.2016.04.007
引用本文: 杨振华, 叶亮, 方苏榕, 谷伟. 免疫组织化学法检测肺癌患者EGFR敏感突变的临床研究[J]. 肿瘤防治研究, 2016, 43(4): 272-276. DOI: 10.3971/j.issn.1000-8578.2016.04.007
YANG Zhenhua, YE Liang, FANG Surong, GU Wei. Clinical Significance of Immunohistochemistry in Detecting Epidermal Growth Factor Receptor Mutation in Lung Cancer[J]. Cancer Research on Prevention and Treatment, 2016, 43(4): 272-276. DOI: 10.3971/j.issn.1000-8578.2016.04.007
Citation: YANG Zhenhua, YE Liang, FANG Surong, GU Wei. Clinical Significance of Immunohistochemistry in Detecting Epidermal Growth Factor Receptor Mutation in Lung Cancer[J]. Cancer Research on Prevention and Treatment, 2016, 43(4): 272-276. DOI: 10.3971/j.issn.1000-8578.2016.04.007

免疫组织化学法检测肺癌患者EGFR敏感突变的临床研究

基金项目: 南京市科学技术委员会2013年度面上项目(H201341)
详细信息
    作者简介:

    杨振华(1974-),男,博士,副主任医师,主要从事肺部肿瘤的早期诊断及晚期肺癌的综合治疗

    通讯作者:

    谷伟,E-mail: njguwei@163.com

  • 中图分类号: R734.2

Clinical Significance of Immunohistochemistry in Detecting Epidermal Growth Factor Receptor Mutation in Lung Cancer

  • 摘要: 目的 探讨免疫组织化学法(immunohistochemistry, IHC)检测EGFR突变的应用价值。方法 通过针对delE746-A750、L858R突变的特异性抗体检测肺癌患者EGFR突变状态,并且与DNA直接测序法进行对照。结果 136例标本中IHC评分0分的48例,其中DNA直接测序法检测突变的3例、评分1+的53例中突变的11例、2+的27例中突变的22例、 3+者中的8例均存在EGFR突变。以≤1+为阴性,≥2+为阳性,敏感度为71.43%,特异性为94.68%,阳性预测值(positive predictive value, PPV)为85.71%,阴性预测值(negative predictive value, NPV)为88.12%,κ值为0.683。结论 免疫组织化学检测EGFR突变应以评分≤1+为阴性,≥2+为阳性来评估EGFR突变状态。

     

    Abstract: Objective To explore the clinical value of immunohistochemistry (IHC) in screening the epidermal growth factor receptor(EGFR) mutation in lung cancer patients. Methods The two mutationspecific antibodies targeting the delE746-A750 mutation in exon 19 and L858R mutation in exon 21 were used for IHC, and the method of direct DNA sequencing was served as control. Results In this investigation, we found 3(6.25%) cases scored 0, 11(20.75%) scored 1, 22(81.48%) scored 2, 8(100%) scored 3 assessed by IHC existed EGFR mutation in direct DNA sequencing analysis. When score≤1+ was considered to be negative and score≥2+ to be positive, the agreement between the two different detection methods was highest(κ=0.683), which the sensitivity was 71.43% and specificity was 94.68%. In the same time, the positive predictive value (PPV) was 85.71% and the negative predictive value (NPV) was 88.12%. Conclusion The score of EGFR mutation assessed by IHC≤1 is considered to be negative mutation whereas score≥2 to be positive mutation.

     

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出版历程
  • 收稿日期:  2015-11-22
  • 修回日期:  2016-01-22
  • 刊出日期:  2016-04-24

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