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IWR-1对人肝癌细胞株Hep3B的增殖及Wnt/β-catenin信号通路的抑制作用

时汀, 张建淮

时汀, 张建淮. IWR-1对人肝癌细胞株Hep3B的增殖及Wnt/β-catenin信号通路的抑制作用[J]. 肿瘤防治研究, 2016, 43(3): 207-210. DOI: 10.3971/j.issn.1000-8578.2016.03.008
引用本文: 时汀, 张建淮. IWR-1对人肝癌细胞株Hep3B的增殖及Wnt/β-catenin信号通路的抑制作用[J]. 肿瘤防治研究, 2016, 43(3): 207-210. DOI: 10.3971/j.issn.1000-8578.2016.03.008
SHI Ting, ZHANG Jianhuai. Inhibitory Effects of IWR-1 on Hepatic Carcinoma Hep3B Cells Proliferation and Wnt/β-catenin Signaling Pathway[J]. Cancer Research on Prevention and Treatment, 2016, 43(3): 207-210. DOI: 10.3971/j.issn.1000-8578.2016.03.008
Citation: SHI Ting, ZHANG Jianhuai. Inhibitory Effects of IWR-1 on Hepatic Carcinoma Hep3B Cells Proliferation and Wnt/β-catenin Signaling Pathway[J]. Cancer Research on Prevention and Treatment, 2016, 43(3): 207-210. DOI: 10.3971/j.issn.1000-8578.2016.03.008

IWR-1对人肝癌细胞株Hep3B的增殖及Wnt/β-catenin信号通路的抑制作用

基金项目: 淮安市科技局资助项目(HAP201303)
详细信息
    作者简介:

    时汀(1990-),男,硕士在读,主要从事肝癌的综合治疗研究

    通讯作者:

    张建淮,E-mail: jh_z02@hotmail.com

  • 中图分类号: R735.7

Inhibitory Effects of IWR-1 on Hepatic Carcinoma Hep3B Cells Proliferation and Wnt/β-catenin Signaling Pathway

  • 摘要: 目的 探讨IWR-1对人肝癌细胞株Hep3B细胞增殖的影响及可能的机制。方法 用不同浓度IWR-1(2、4、8、16μmol/L)处理Hep3B细胞,CCK-8法检测细胞生长抑制率;流式细胞仪检测细胞周期变化及细胞凋亡率;Western blot法检测细胞中蛋白表达的变化,实时定量RT-PCR检测β-catenin和c-myc mRNA表达的变化。结果 IWR-1对人肝癌Hep3B细胞的生长抑制作用呈现剂量和时间依赖性(P<0.01)。流式细胞术结果显示,Hep3B细胞的细胞周期呈现明显的G0/G1期阻滞,且细胞凋亡率明显升高(P<0.01),呈现剂量依赖性。IWR-1可引起β-catenin和c-myc mRNA表达下降,β-catenin、c-myc蛋白表达下降,而Axin 1和p-β-catenin蛋白表达上升。结论 IWR-1可以抑制人肝癌细胞株Hep3B的增殖,其机制可能是通过抑制Wnt/β-catenin通路来实现。

     

    Abstract: Objective To investigate the effects of IWR-1 on proliferation and the possible mechanism of human hepatic carcinoma cell lines Hep3B. Methods Hep3B cells were treated with different concentration of IWR-1(2,4,8,16μmol/L). Cell growth inhibition rates were determined by CKK-8 assay. Cell cycle and apoptosis rate were analyzed by flow cytometry(FCM). The expression levels of proteins were detected by Western blotting. β-catenin and c-myc mRNA expression were determined by real time polymerase chain reaction(RTPCR). Results The proliferation rate of Hep3B cells in the IWR-1 treated group decreased in a time- and concentration- dependent manner(P<0.01). FCM results showed that the proportion of cells in G0/G1 phase were increased while the apoptosis rate of Hep3B cells was decreased in a concentration-dependent manner(P<0.01). IWR-1 could down-regulate β-catenin and c-myc mRNA levels, down-regulate β-catenin and c-myc mRNA protein levels, but significantly up-regulate Axin 1 and p-β-catenin protein levels. Conclusion IWR-1 can inhibite proliferation of Hep3B cells through inhibiting Wnt/β-catenin signaling pathway.

     

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出版历程
  • 收稿日期:  2015-07-04
  • 修回日期:  2015-09-24
  • 刊出日期:  2016-03-24

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