Abstract: Objective To detect the transcriptional levels of mitochondrial DNA(mtDNA) ATPase6 and ATPase8 in breast invasive ductal carcinoma and to investigate their significance in the occurrence and development of breast invasive ductal carcinoma. Methods The 25 cases of breast invasive ductal carcinoma tissues and adjacent normal tissues were involved in this investigation. The mtDNA ATPase6 and ATPase8 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR), to observe the differences and their association with the clinical parameters. Results The mtDNA ATPase6 mRNA expression levels in cancer tissues were higher than those in adjacent normal tissues (0.77±0.12) vs. (0.32±0.07), P<0.01. The mtDNA ATPase8 mRNA expression levels in cancer tissues were higher than those in adjacent normal tissues (0.82±0.12) vs. (0.54±0.06), P<0.01. The mtDNA ATPase6 and ATPase8 mRNA expression levels in the lymph node metastasis positive group were higher than those in lymph node metastasis negative group (0.88±0.21) vs. (0.67±0.11), P<0.05 and (0.91±0.10) vs. (0.72±0.08), P<0.05; those in TNM stageⅡ group were higher than those in TNM stageⅠgroup (0.72±0.24) vs. (0.51±0.13), P<0.05 and (0.79±0.15) vs. (0.54±0.09), P<0.05; those in TNM stageⅢ group were higher than those in TNM stageⅠgroup(0.79±0.18) vs. (0.51±0.13), P<0.01 and (0.87±0.12) vs. (0.54±0.09), P<0.01. Conclusion The transcriptional levels of mitochondrial DNA ATPase6 and ATPase8 are increased in breast invasive ductal carcinoma tissues, and could play certain roles in the occurrence and metastasis of carcinoma.