Transcriptional Levels of Mitochondrial DNA ATPase6 and ATPase8 in Breast Invasive Ductal Carcinoma and Related Significance
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摘要: 目的 检测线粒体DNA（mitochondrial DNA, mtDNA）在乳腺浸润性导管癌中的转录情况，探讨其在肿瘤发生及发展中的作用。方法 采用反转录-聚合酶链反应（RT-PCR），分别对25例乳腺浸润性导管癌患者的癌组织及癌旁组织的mtDNA ATPase6 与ATPase8 mRNA进行检测，并观察其差异以及与相关临床病理参数的关系。结果 mtDNA ATPase6 mRNA的相对表达量在癌组织中明显高于癌旁组织（0.77±0.12 vs. 0.32±0.07, P<0.01）；mtDNA ATPase8 mRNA的相对表达量在癌组织中明显高于癌旁组织（0.82±0.12 vs. 0.54±0.06, P<0.01）；淋巴结转移阳性组的ATPase6和ATPase8 mRNA相对表达量均高于淋巴结转移阴性组，分别为（0.88±0.21）vs.（0.67±0.11）和（0.91±0.10）vs.（0.72±0.08），差异均有统计学意义（P均<0.05）；TNM分期Ⅱ期组的ATPase6和ATPase8 mRNA相对表达量均高于Ⅰ期组，分别为（0.72±0.24）vs.（0.51±0.13）和（0.79±0.15）vs.（0.54±0.09），差异均有统计学意义（P均<0.05）；Ⅲ期组的ATPase6和ATPase8 mRNA相对表达量均明显高于Ⅰ期组，分别为（0.79±0.18）vs.（0.51±0.13）和（0.87±0.12）vs.（0.54±0.09），差异均有统计学意义（P<0.01）。结论 乳腺浸润性导管癌组织中mtDNA ATPase6与ATPase8的转录水平上调，并且可能在肿瘤的发生及转移过程起到一定的作用。
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关键词:
- 乳腺浸润性导管癌 /
- 线粒体DNA /
- 反转录-聚合酶链反应
Abstract: Objective To detect the transcriptional levels of mitochondrial DNA(mtDNA) ATPase6 and ATPase8 in breast invasive ductal carcinoma and to investigate their significance in the occurrence and development of breast invasive ductal carcinoma. Methods The 25 cases of breast invasive ductal carcinoma tissues and adjacent normal tissues were involved in this investigation. The mtDNA ATPase6 and ATPase8 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR), to observe the differences and their association with the clinical parameters. Results The mtDNA ATPase6 mRNA expression levels in cancer tissues were higher than those in adjacent normal tissues [(0.77±0.12) vs. (0.32±0.07), P<0.01]. The mtDNA ATPase8 mRNA expression levels in cancer tissues were higher than those in adjacent normal tissues [(0.82±0.12) vs. (0.54±0.06), P<0.01]. The mtDNA ATPase6 and ATPase8 mRNA expression levels in the lymph node metastasis positive group were higher than those in lymph node metastasis negative group [(0.88±0.21) vs. (0.67±0.11), P<0.05 and (0.91±0.10) vs. (0.72±0.08), P<0.05]; those in TNM stageⅡ group were higher than those in TNM stageⅠgroup [(0.72±0.24) vs. (0.51±0.13), P<0.05 and (0.79±0.15) vs. (0.54±0.09), P<0.05]; those in TNM stageⅢ group were higher than those in TNM stageⅠgroup[(0.79±0.18) vs. (0.51±0.13), P<0.01 and (0.87±0.12) vs. (0.54±0.09), P<0.01]. Conclusion The transcriptional levels of mitochondrial DNA ATPase6 and ATPase8 are increased in breast invasive ductal carcinoma tissues, and could play certain roles in the occurrence and metastasis of carcinoma. -
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