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阿霉素对不同转移能力肝癌细胞及肝癌干细胞相关基因表达的影响

袁鹏, 岳天华, 肖艳华, 朱梁军, 李晟, 陈宝安, 王传伟

袁鹏, 岳天华, 肖艳华, 朱梁军, 李晟, 陈宝安, 王传伟. 阿霉素对不同转移能力肝癌细胞及肝癌干细胞相关基因表达的影响[J]. 肿瘤防治研究, 2015, 42(12): 1192-1197. DOI: 10.3971/j.issn.1000-8578.2015.12.005
引用本文: 袁鹏, 岳天华, 肖艳华, 朱梁军, 李晟, 陈宝安, 王传伟. 阿霉素对不同转移能力肝癌细胞及肝癌干细胞相关基因表达的影响[J]. 肿瘤防治研究, 2015, 42(12): 1192-1197. DOI: 10.3971/j.issn.1000-8578.2015.12.005
YUAN Peng, YUE Tianhua, XIAO Yanhua, ZHU Liangjun, LI Sheng, CHEN Baoan, WANG Chuanwei. Influence of Adriamycin on Human Hepatocellular Carcinoma Cells with Different Metastatic Abilities and Expression of Cancer Stem Cell Related Genes[J]. Cancer Research on Prevention and Treatment, 2015, 42(12): 1192-1197. DOI: 10.3971/j.issn.1000-8578.2015.12.005
Citation: YUAN Peng, YUE Tianhua, XIAO Yanhua, ZHU Liangjun, LI Sheng, CHEN Baoan, WANG Chuanwei. Influence of Adriamycin on Human Hepatocellular Carcinoma Cells with Different Metastatic Abilities and Expression of Cancer Stem Cell Related Genes[J]. Cancer Research on Prevention and Treatment, 2015, 42(12): 1192-1197. DOI: 10.3971/j.issn.1000-8578.2015.12.005

阿霉素对不同转移能力肝癌细胞及肝癌干细胞相关基因表达的影响

基金项目: 四川省卫生厅科研课题(120058)
详细信息
    作者简介:

    袁鹏(1973-),男,硕士,副主任医师,主要从事肿瘤的化疗、介入等综合治疗

    通讯作者:

    陈宝安,E-mail: cba8888@hotmail.com

  • 中图分类号: R965;R735.7

Influence of Adriamycin on Human Hepatocellular Carcinoma Cells with Different Metastatic Abilities and Expression of Cancer Stem Cell Related Genes

  • 摘要: 目的 探讨阿霉素(adriamycin, ADM)对不同转移能力肝癌细胞及肝癌干细胞相关基因表达的影响。方法 利用MTT法检测ADM对人肝癌细胞系MHCC97-L、HCCLM3的抑制作用,计算各自的半数致死浓度(median lethal dose, LD50);Western blot法检测ADM作用下人肝癌细胞系MHCC97-L、HCCLM3中干细胞基因Nanog、Oct-4、Sox2、ARID1A、Wnt5b的表达,并分析干细胞基因在两种细胞中表达变化的差异。结果 ADM浓度越高,对人肝癌细胞的抑制作用越明显,其对MHCC97-L和HCCLM3的半数致死浓度分别为0.4212 μmol/L和0.5249 μmol/L;随着ADM(LD50)作用时间的延长,MHCC97-L和HCCLM3中Wnt5b的表达水平先升高后降低,Nanog蛋白的表达先降低后升高,Sox2在HCCLM3中表达水平逐渐升高。Wnt5b和Nanog 4 h内在低转移能力肝癌细胞系MHCC97-L中的表达变化均值均小于其在高转移能力肝癌细胞系HCCLM3时的表达变化均值,差异有统计学意义(P<0.05)。结论 ADM可促进MHCC97-L和HCCLM3细胞的凋亡,且对MHCC97-L的作用强于HCCLM3;干细胞相关基因Wnt5b与肝癌细胞的恶性程度呈负相关;Nanog和Sox2均与肿瘤的转移密切相关,且Nanog和Sox2可能与肝癌耐药密切相关。

     

    Abstract: Objective To investigate the influence of Adriamycin (ADM) on human hepatocellular carcinoma(HCC) cells with different metastatic abilities and related gene expressions of HCC stem cell. Methods MTT was used to detect the inhibition effect of ADM on human HCC cell lines MHCC97-L and HCCLM3, to compute the LD50 concentration. Western blot was used to detect the expression levels of Nanog, Oct-4, Sox2, ARID1 and Wnt5b in human HCC cell lines MHCC97-L and HCCLM3, and to analyze the difference of their expression levels between the two different cell lines. Results With the increased ADM level, the inhibition effect was improved gradually, and the LD50 to MHCC97-L and HCCLM3 cell lines were 0.4212 and 0.5249μmol/L, respectively. As the action time of ADM (LD 50) prolonged, the level of Wnt5b was raised at first and then declined, while the level of Nanog was declined at first and then raised in both MHCC97-L and HCCLM3 cell lines. The level of Sox2 was improved in HCCLM3 cells in a time-dependent manner. The levels of Wnt5b and Nanog were lower in MHCC97-L cells than those in HCCLM3 cells within 4h (P<0.05). Conclusion ADM could promote the apoptosis rate of MHCC97-L and HCCLM3 cell lines, and the influence was much better in MHCC97-L cells than that in HCCLM3 cells; Wnt5b is negatively correlated with the tumors malignancy degree; Nanog and Sox2 are closely related with the metastasis of the tumor, which may related with the drug resistance of HCC.

     

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出版历程
  • 收稿日期:  2014-11-26
  • 修回日期:  2014-11-26
  • 刊出日期:  2015-12-24

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