Expression of Costimulator B7-H1 Protein in Gallbladder Carcinoma Tissues and Its Relationship with Prognosis
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摘要: 目的 观察B7-H1蛋白在胆囊癌中的表达；探讨其在胆囊癌的发生发展中的作用及其与预后的关系。方法 用免疫组织化学SP法检测B7-H1蛋白在60例胆囊癌组织中的表达；另取癌旁非肿瘤组织20例及胆囊腺瘤性息肉20例作为对照组。结果 胆囊癌组中B7-H1蛋白的表达明显高于癌旁非肿瘤组织组（P=0.003）和胆囊腺瘤性息肉组（P=0.035）；B7-H1蛋白在Nevin（Ⅳ+Ⅴ）期、TNM（Ⅲ+Ⅳ）期、有淋巴结转移组中的表达明显高于Nevin（Ⅰ+Ⅱ+Ⅲ）期（P=0.041）、TNM（Ⅰ+Ⅱ）期（P=0.049）、无淋巴结转移组（P=0.000）；与性别、年龄及肿瘤分化程度无关（P>0.05） 。B7-H1蛋白阳性表达组的三年生存期明显短于阴性表达者（P=0.029）。结论 B7-H1蛋白在胆囊癌中高表达导致预后不良，提示B7-H1蛋白在胆囊癌的发生发展和浸润转移中起作用。Abstract: Objective To investigate the expression of B7-H1 protein in gallbladder carcinoma tissues and to explore its role in the carcinogenesis and progression of gallbladder carcinoma. Methods The expression of B7-H1 protein in 60 cases of gallbladder carcinoma tissues were detected by immunohistochemistry(SP) method; 20 cases of corresponding non-tumor tissues and 20 cases of gallbladder adenoma polyp were served as control groups. Results The expression of B7-H1 protein in gallbladder carcinoma group was significantly higher than that in corresponding non-tumor tissues group(P=0.003) and gallbladder adenoma polyp group(P=0.035); the B7-H1 protein expression levels in Nevin(Ⅳ+Ⅴ) stage, TNM(Ⅲ+Ⅳ) stage and lymph node metastasis group were significantly higher than those in Nevin(Ⅰ+Ⅱ+Ⅲ) stage (P=0.041), TNM(Ⅰ+ Ⅱ) stage(P=0.049) and lymph node metastasis-free group(P=0.000); but the expression of B7-H1 protein had no relationship with age, gender or tumor differentiation(P>0.05). The three-year survival time of the patients with positive expression of B7-H1 protein was significant lower than those with negative expression of B7-H1 protein(P=0.029). Conclusion The overexpression of B7-H1 protein results in poor prognosis, indicating that B7-H1 protein plays role in the development, invasion and metastasis of gallbladder carcinoma.
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Key words:
- B7-H1 protein /
- Gallbladder carcinoma /
- Immunohistochemistry /
- Prognosis
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