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HOXB7对人非小细胞肺癌A549细胞增殖的影响

李劲松, 韩亮, 于洋, 刘勇

李劲松, 韩亮, 于洋, 刘勇. HOXB7对人非小细胞肺癌A549细胞增殖的影响[J]. 肿瘤防治研究, 2015, 42(11): 1119-1123. DOI: 10.3971/j.issn.1000-8578.2015.11.013
引用本文: 李劲松, 韩亮, 于洋, 刘勇. HOXB7对人非小细胞肺癌A549细胞增殖的影响[J]. 肿瘤防治研究, 2015, 42(11): 1119-1123. DOI: 10.3971/j.issn.1000-8578.2015.11.013
LI Jinsong, HAN Liang, YU Yang, LIU Yong. HOXB7 Regulates Proliferation of Non-small Cell Lung Cancer Cell A549[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1119-1123. DOI: 10.3971/j.issn.1000-8578.2015.11.013
Citation: LI Jinsong, HAN Liang, YU Yang, LIU Yong. HOXB7 Regulates Proliferation of Non-small Cell Lung Cancer Cell A549[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1119-1123. DOI: 10.3971/j.issn.1000-8578.2015.11.013

HOXB7对人非小细胞肺癌A549细胞增殖的影响

基金项目: 徐州市科技项目(XM13B053、XZZD1237)
详细信息
    作者简介:

    李劲松(1970-),男,硕士,副主任医师,主要从事恶性肿瘤的研究

    通讯作者:

    刘勇,E-mail:lyly.7011@163.com

  • 中图分类号: R730.5

HOXB7 Regulates Proliferation of Non-small Cell Lung Cancer Cell A549

  • 摘要: 目的 研究HOXB7在人非小细胞肺癌组织及细胞系中的表达水平,HOXB7表达水平改变对非小细胞肺癌细胞增殖的影响。方法 通过Real-time PCR、免疫组织化学技术检测人非小细胞肺癌及细胞系中HOXB7的表达水平,根据病理资料初步分析HOXB7与非小细胞肺癌预后的关系。通过转染si-RNA抑制HOXB7的表达水平,并通过定量PCR检测转染效率。利用流式细胞术检测抑制HOXB7对A549细胞增殖能力的影响。结果 相对正常肺组织及细胞,在非小细胞肺癌组织和细胞系中HOXB7的表达出现了显著上调,高表达HOXB7的NSCLC患者整体生存率较差。转染si-RNA能显著抑制HOXB7表达水平和人非小细胞肺癌A549细胞的增殖能力。结论 非小细胞肺癌组织及细胞系中HOXB7的表达显著上调。

     

    Abstract: Objective To investigate the expression level of HOXB7 in non-small cell lung cancer (NSCLC) tissues and cell lines, and the effect of HOXB7 expression on the proliferation of NSCLC cells A549. Methods Real-time quantitative PCR and immunohistochemical method were performed to detect the relative expression of HOXB7 in NSCLC tissues and cell lines. Si-HOXB7 were transfected into A549 cells to downregulate HOXB7 expression, and qPCR was used to test the transfection efficiency. Pathological data were used to analyze the relationship between HOXB7 expression and the prognosis of NSCLC patients. MTT and flow cytometry analysis were performed to detect the effect of HOXB7 on the proliferation of NSCLC cells. Results HOXB7 were highly expressed both in NSCLC tissues and cell lines, compared with their corresponding normal tissues and cell lines. NSCLC patients with high HOXB7 expression showed poor overall survival. si-RNA transfection could significantly decreased HOXB7 expression in NSCLC cells A549, and down-regulating of HOXB7 expression could inhibit NSCLC cells A549 proliferation. Conclusion Upregulated HOXB7 could promote NSCLC cells proliferation.

     

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出版历程
  • 收稿日期:  2014-11-19
  • 修回日期:  2015-05-19
  • 刊出日期:  2015-11-24

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