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结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义

刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红

刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红. 结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J]. 肿瘤防治研究, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
引用本文: 刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红. 结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J]. 肿瘤防治研究, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
shu
LIU Jie, PENG Wei, WU Zhihui, LIU Huai, SONG Haipeng, DENG Zhenghao, XU Xiaohong. Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
Citation: LIU Jie, PENG Wei, WU Zhihui, LIU Huai, SONG Haipeng, DENG Zhenghao, XU Xiaohong. Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
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结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义

  • 1. 412000 株洲，湖南中医药高等专科学校基础医学部；2. 412000 株洲，株洲市中心医院心胸外科；3. 410000 长沙，中南大学湘雅医学院病理学系

基金项目: 湖南省卫生厅科研基金课题（B2013-067）
详细信息
    作者简介:

    刘洁（1981-），女，硕士，讲师，主要从事消化道肿瘤分子病理研究

  • 中图分类号: R735.3+5

Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance

  • 1. Department of Basic Medical, Hu'nan Traditional Chinese Medical College, Zhuzhou 412000, China; 2. Department of Cardiothoracic Surgery, The Central Hospital of Zhuzhou City, Zhuzhou 412000, China; 3. Department of Pathology, Xiangya School of Medicine,Central South University, Changsha 410000, China

摘要

    摘要
  • 摘要: 目的 探讨结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义。方法 实时荧光定量聚合酶链反应法（real-time quantitative PCR, qRT-PCR）检测结肠癌患者与健康对照组血浆中miR-21 的表达情况，以及与临床病理参数的关系；另外检测结肠癌患者手术前一周、手术后一周和术后一月血浆中miR-21的表达水平变化。 结果 40例结肠癌患者手术前血浆中miR-21表达水平较对照组差异具有统计学意义（P<0.05）；并与肿瘤的分化程度、临床分期、淋巴结转移及远处转移有关（P <0.05），而与年龄和性别无相关性（P>0.05）；与术前相比，术后一周和一月血浆中miR-21的表达明显降低，差异有统计学意义（P<0.05）。 结论 结肠癌患者血浆中miR-21的表达情况，可作为结肠癌潜在的生物标志物，有望成为一种新的有效辅助诊断结肠癌及判断预后的方法。

     

    Abstract: Objective To investigate the expression of plasma miR-21 in patients with colon carcinoma before and after operation and its clinical significance. Methods The plasma miR-21 expression and its relationship with clinical pathological parameters were measured by quantitative real-time PCR(qRT-PCR). In addition, the expression levels of plasma miR-21 in colon cancer patients were measured one week before operation, one week and one month after the operation. Results The plasma miR-21 expression in 40 colon cancer patients before operation had significant difference compared with control group(P<0.05), related to the degree of tumor differentiation, clinical stage, lymph node metastasis and distant metastasis(P<0.05), but age or sex(P>0.05). The plasma miR-21 expression one week and one month after the operation were significantly decreased, compared with that before operation(P<0.05). Conclusion The plasma miR-21 expression may be used as a potential biomarker for colon cancer, to be a new effective auxiliary method for the diagnosis and prognosis of colon cancer patients.

     

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    • 参考文献(12)
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出版历程
  • 收稿日期:  2014-09-08
  • 修回日期:  2015-04-17
  • 刊出日期:  2015-08-24

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