高级搜索

结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义

刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红

刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红. 结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J]. 肿瘤防治研究, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
引用本文: 刘洁, 彭微, 吴稚晖, 刘怀, 宋海鹏, 邓征浩, 徐筱红. 结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J]. 肿瘤防治研究, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
shu
LIU Jie, PENG Wei, WU Zhihui, LIU Huai, SONG Haipeng, DENG Zhenghao, XU Xiaohong. Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
Citation: LIU Jie, PENG Wei, WU Zhihui, LIU Huai, SONG Haipeng, DENG Zhenghao, XU Xiaohong. Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 794-796. DOI: 10.3971/j.issn.1000-8578.2015.08.009
shu

结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义

  • 1. 412000 株洲，湖南中医药高等专科学校基础医学部；2. 412000 株洲，株洲市中心医院心胸外科；3. 410000 长沙，中南大学湘雅医学院病理学系

基金项目: 湖南省卫生厅科研基金课题（B2013-067）
详细信息
    作者简介:

    刘洁（1981-），女，硕士，讲师，主要从事消化道肿瘤分子病理研究

  • 中图分类号: R735.3+5

Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance

  • 1. Department of Basic Medical, Hu'nan Traditional Chinese Medical College, Zhuzhou 412000, China; 2. Department of Cardiothoracic Surgery, The Central Hospital of Zhuzhou City, Zhuzhou 412000, China; 3. Department of Pathology, Xiangya School of Medicine,Central South University, Changsha 410000, China

摘要

    摘要
  • 摘要: 目的 探讨结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义。方法 实时荧光定量聚合酶链反应法（real-time quantitative PCR, qRT-PCR）检测结肠癌患者与健康对照组血浆中miR-21 的表达情况，以及与临床病理参数的关系；另外检测结肠癌患者手术前一周、手术后一周和术后一月血浆中miR-21的表达水平变化。 结果 40例结肠癌患者手术前血浆中miR-21表达水平较对照组差异具有统计学意义（P<0.05）；并与肿瘤的分化程度、临床分期、淋巴结转移及远处转移有关（P <0.05），而与年龄和性别无相关性（P>0.05）；与术前相比，术后一周和一月血浆中miR-21的表达明显降低，差异有统计学意义（P<0.05）。 结论 结肠癌患者血浆中miR-21的表达情况，可作为结肠癌潜在的生物标志物，有望成为一种新的有效辅助诊断结肠癌及判断预后的方法。

     

    Abstract: Objective To investigate the expression of plasma miR-21 in patients with colon carcinoma before and after operation and its clinical significance. Methods The plasma miR-21 expression and its relationship with clinical pathological parameters were measured by quantitative real-time PCR(qRT-PCR). In addition, the expression levels of plasma miR-21 in colon cancer patients were measured one week before operation, one week and one month after the operation. Results The plasma miR-21 expression in 40 colon cancer patients before operation had significant difference compared with control group(P<0.05), related to the degree of tumor differentiation, clinical stage, lymph node metastasis and distant metastasis(P<0.05), but age or sex(P>0.05). The plasma miR-21 expression one week and one month after the operation were significantly decreased, compared with that before operation(P<0.05). Conclusion The plasma miR-21 expression may be used as a potential biomarker for colon cancer, to be a new effective auxiliary method for the diagnosis and prognosis of colon cancer patients.

     

    • HTML全文
    • 参考文献(12)
  • [1] Sun JB, Xia L. Detection of plasma circulating miR-21 of colorectal carcinoma patients by a quantitative real-time PCR[J]. Lin Chuang Xue Ye Xue Za Zhi (Shu Xue Yu Jian Yan Ban), 20 11, 24(4): 444-7. [孙建斌, 夏琳. 实时荧光定量检测大肠癌患 者血浆miR-21的表达[J]. 临床血液学杂志（输血与检验版）, 20 11, 24(4): 444-7.]
    [2] Slaby O, Svoboda M, Fabian P, et al. Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer[J]. Oncology, 2007, 72(5-6): 39 7-402.
    [3] Faltejskova P, Besse A, Sevcikova S, et al. Clinical correlations of miR-21 expression in colorectal cancer patients and effects of its inhibition on DLD1 colon cancer cells[J]. Int J Colorectal Dis, 20 12, 27(11): 1401-8.
    [4] Scott GK, Goga A, Bhaumik D, et al. Coordinate suppression of ERBB2 and ERBB3 by enforced expression of micro-RNA miR- 12 5b[J]. J Biol Chem, 2007, 282(2): 1479-86.
    [5] Chan SH, Wu CW, Li AF, et al. miR-21 microRNA expression in human gastric carcinomas and its clinical association[J]. Anticancer Res, 2008, 28(2A): 907-11.
    [6] Lu X, Su J, Gao W, et al. Expression of microRNA in NSCLC with drug-resistant cell line A549 /DDP[J]. Zhonghua Zhong Liu Fang Zhi Za Zhi, 2010, 17(9): 659-63. [陆晓, 孙婧, 高雯, 等. 非小细 胞肺癌耐药细胞株A549/DDP中microRNA表达的初步研究[J]. 中华肿瘤防治杂志, 2010, 17(9): 659-63.]
    [7] Selcuklu SD, Donoghue MT, Spillane C. miR-21 as a key regulator of oncogenic processes[J]. Biochem Soc Trans, 2009, 37(4): 918-25.
    [8] Zhong Q, Fang JG, Huang ZG, et al. Expression of miRNA in laryngeal squamous cell carcinoma tissues[J]. Zhonghua Zhong Liu Fang Zhi Za Zhi, 2010, 17(14): 1073-6. [钟琦, 房居高, 黄志 刚, 等. 喉鳞状细胞癌组织miRNA表达的初步研究[J]. 中华肿 瘤防治杂志, 2010, 17(14): 1073-6.]
    [9] Liang Y, Wu JX. Progress of miR-21 and tumor[J]. Zhonghua Zhong Liu Fang Zhi Za Zhi, 2012, 19(12): 949-52. [梁燕, 吴建新. miR-21与肿瘤研究进展[J]. 中华肿瘤防治杂志, 2012, 19(12): 94 9-52.]
    [10] Basati G, Emami Razavi A, Abdi S, et al. Elevated level of microRNA-21 in the serum of patients with colorectal cancer[J]. Med Oncol, 2014, 31(10): 205.
    [11] Masuda S, Izpisua Belmonte JC. Serum miR-21 as a diagnostic and prognostic biomarker in colorectal cancer[J]. J Natl Cancer Inst, 2014, 106(3): djt457.
    [12] Wang B, Zhang Q. The expression and clinical significance of circulating microRNA-21 in serum of five solid tumors[J]. J Cancer Res Clin Oncol, 2012, 138(10): 1659-66.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1304
  • HTML全文浏览量:  338
  • PDF下载量:  451
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-09-08
  • 修回日期:  2015-04-17
  • 刊出日期:  2015-08-24

目录

摘要
HTML全文
    参考文献(12)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量：12442490 今日访问：19713

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭