高级搜索

卵巢癌细胞及组织中P38MAPK信号通路调控uPA蛋白的表达及临床意义

盛梅, 张海燕, 宋冬冬, 邹存华

盛梅, 张海燕, 宋冬冬, 邹存华. 卵巢癌细胞及组织中P38MAPK信号通路调控uPA蛋白的表达及临床意义[J]. 肿瘤防治研究, 2015, 42(08): 789-793. DOI: 10.3971/j.issn.1000-8578.2015.08.008
引用本文: 盛梅, 张海燕, 宋冬冬, 邹存华. 卵巢癌细胞及组织中P38MAPK信号通路调控uPA蛋白的表达及临床意义[J]. 肿瘤防治研究, 2015, 42(08): 789-793. DOI: 10.3971/j.issn.1000-8578.2015.08.008
shu
SHENG Mei, ZHANG Haiyan, SONG Dongdong, ZOU Cunhua. P38MAPK Signaling Pathway is Involved in Expression Regulation of uPA Protein in Ovarian Cancer Cells and Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 789-793. DOI: 10.3971/j.issn.1000-8578.2015.08.008
Citation: SHENG Mei, ZHANG Haiyan, SONG Dongdong, ZOU Cunhua. P38MAPK Signaling Pathway is Involved in Expression Regulation of uPA Protein in Ovarian Cancer Cells and Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 789-793. DOI: 10.3971/j.issn.1000-8578.2015.08.008
shu

卵巢癌细胞及组织中P38MAPK信号通路调控uPA蛋白的表达及临床意义

  • 1. 257000东营，胜利油田中心医院妇产科；2. 257000东营，胜利油田石油管理局妇幼保健站；3. 257000东营，胜利油田中心医院肛肠外科

基金项目: 国家自然科学基金资助项目(30970651)；国家重点实验室基金资助项目(SKLZZ200907)
详细信息
    作者简介:

    盛梅（1967-），女，本科，主任医师，主要从事妇产科肿瘤的临床与研究工作

    通讯作者:

    邹存华，E-mail: zxyyzch1116@163.com

  • 中图分类号: R737.31

P38MAPK Signaling Pathway is Involved in Expression Regulation of uPA Protein in Ovarian Cancer Cells and Tissues

  • 1. Department of Gynaecology and Obstetrics, Shengli Oilfield Central Hospital, Dongying 257000, China; 2. Maternal and Child Health Hospital of Shengli Oilfield, Dongying 257000, China; 3. Department of Anorectal Surgery, Shengli Oilfield Central Hospital, Dongying 257000, China

摘要

    摘要
  • 摘要: 目的 探讨P38MAPK信号通路与uPA在卵巢癌细胞及组织中的表达及临床意义。方法 应用免疫组织化学法检测uPA、P38MAPK、ERK、AKT蛋白在49例卵巢癌组织中的表达，Western blot检测HO8910及HO-8910PM细胞系中uPA、P38MAPK蛋白的表达，特异性抑制剂SB203580阻断P38MAPK信号通路后uPA蛋白表达水平的变化。结果 uPA、P38MAPK、ERK、AKT蛋白在卵巢癌组织中表达阳性率分别为61.22%、57.14%、53.06%、55.10%。uPA与p38MAPK蛋白表达呈正相关（r=0.8645，P=0.001），且与卵巢癌组织的临床病理分期、分化、转移程度有关（P均<0.05），而与患者的年龄、组织学类型无明显相关（P>0.05）。ERK、AKT蛋白表达与卵巢癌淋巴结转移、大网膜转移有关（P均<0.05），而与患者的年龄、组织类型、病理分期无明显关系（P>0.05）。HO-8910PM细胞系中uPA蛋白的表达水平明显高于HO8910，SB203580阻断P38MAPK信号通路后可降低uPA蛋白的表达，且随着SB203580 浓度升高，uPA蛋白表达逐渐降低。P38MAPK及uPA蛋白的表达与卵巢癌的预后显著相关（r=3.897, 11.044, P=0.048, 0.001）。结论 卵巢癌组织中P38MAPK信号通路处于激活状态；该通路的激活可上调uPA的表达，促进卵巢癌的恶性进展；P38MAPK信号通路和uPA可能在卵巢癌侵袭和转移的过程中发挥重要作用。P38MAPK和uPA蛋白有望成为卵巢癌预后评估的重要指标之一。

     

    Abstract: Objective To explore the expression of P38 MAPK signaling pathway and uPA in ovarian cancer and related clinical significance. Methods The expression of uPA, AKT, ERK and P38MAPK were detected in 49 cases of ovarian cancer tissues by immunohistochemistry. The expression of uPA and P38MAPK were detected by Western blot in ovarian cancer cell lines HO8910 and HO-8910PM. We detected the change ofuPA protein expression after P38MAPK signal pathway was cut off by SB203580 specific inhibitor. Results Immunohistochemical method results showed that the positive expression rates of uPA, P38MAPK, ERK and AKT protein were 61.22%, 57.14%, 53.06% and 55.10%, respectively. The expression of uPA was positively correlated with P38MAPK(r=0.8645, P=0.001), and was related with clinicopathologic stage, differentiated degree, lymph node metastasis(all P<0.05), but not related with age or histologic type(P>0.05). The expression of AKT and ERK were related with lymph node metastasis and greater omentum metastasis(P<0.05), but not related with age, histologic type or clinicopathologic stage(P>0.05). The expression of uPA in HO-8910PM cell line was higher than that in ovarian cancer cell lines HO8910, and the expression of uPA was decreased when P38MAPK signal pathway was cut off by SB203580, in a concentration-dependent manner.The expression of P38MAPK and uPA were significantly correlated to the prognosis of ovarian cancer patients(r=3.897, 11.044, P=0.048, 0.001). Conclusion P38MAPK signal pathway is activated in ovarian cancer tissues. The activated p38MAPK signal pathway could upregulate the expression of uPA, which may contribute to the development of ovarian cancer. P38MAPK signal pathway and uPA may play an important role in the invasion and metastasis of ovarian cancer. P38MAPK and uPA might help to evaluate the prognosis of ovarian cancer patients.

     

    • HTML全文
    • 参考文献(13)
  • [1] Qin XL, Jing P, Zhao QM. Diagnosis and treatment advances in recyrrent ovarian cancer[J]. Zhong Liu Fang Zhi Yan Jiu, 2010, 37 (2): 236-8. [秦晓黎, 金平, 赵全明. 复发性卵巢癌治疗进[J]. 肿瘤防治研究, 2010, 37(2): 236-8.]
    [2] Liu B, Kuang AR. Genetic aAlterations in MAPK/ERK and PI3K/ Akt signaling pathways and the generation, progression, diagnosis and therapy of thyroid cancer[J]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 2012, 29(6): 1221-5. [刘斌, 匡安仁. MAPK/ERK和 PI3K/Akt信号通道的基因变异与甲状腺癌的发生发展及诊治[J]. 生物医学工程学杂志, 2012, 29(6): 1221-5.]
    [3] Feng B, Xiong GY, Wang Y, et al. Significance of maspin and uPA expression in colon cancer[J]. Zhongguo Lin Chuang Yan Jiu, 20 11, 24(10): 874-6. [冯波, 熊观瀛, 王芸, 等. 结肠癌组织中 maspin及uPA基因的表达与临床意义[J]. 中国临床研究, 2011, 24 (10): 874-6.]
    [4] Wang YQ, Han DL, Bo AH, et al. Significances of EGFR, Survivin and uPA expression and their correlation[J]. Zhongguo Fu You Bao Jian, 2010, 25(35): 5297-9. [王永清, 韩德兰, 博爱华, 等. EGFR、Survivin和uPA在乳腺癌表达的意义及其相关性[J]. 中 国妇幼保健, 2010, 25(35): 5297-9.]
    [5] Cui TT, Xi SH. The relationship betwwen MAPK signal transduction pathway and cancer[J]. Shi Yong Zhong Liu Za Zhi, 20 13, 28(5): 550-2. [崔婷婷, 席淑华. MAPK信号传导通路与肿 瘤的发生[J]. 实用肿瘤杂志, 2013, 28(5): 550-2.]
    [6] Dong H, Lin YP, Ying XX, et al. The expression of uPA and PA-1 in patients with breast cancer and their clinical significance[J]. Zhong Liu, 2013, 33(4): 361-7. [董欢, 林燕苹, 应学翔, 等. 乳 腺癌组织中uPA和PAI-1的表达及其临床意义[J]. 肿瘤, 2013, 33 (4): 361-7.]
    [7] Uhrin P, Breuss JM. uPAR: a modulator of VEGF-induced angiogenesis[J]. Cell Adh Migr, 2013, 7(1): 23-6.
    [8] Chen HF, Deng HY. Cross-talking betwween JAK/STAT3 and MAPK/ERK signaling transduction pathways on invasion and metastasis of the human breast cancer cells[J]. Zhong Liu Fang Zhi Yan Jiu, 2009, 36(4): 293-7. [陈红芳, 邓华瑜. JAK/STAT3和 MAPK/ERK在乳腺癌细胞侵袭转移中的交互作用及意义[J]. 肿瘤防治研究, 2009, 36(4): 293-7.]
    [9] Ge SX, Yao J, Ding ZX, et al. Experssion and relationship of ERβ and ERK1/2 in epithelial ovarian cancer tissues[J]. Zhonghua Zhong Liu Fang Zhi Za Zhi, 2013, 20(3): 206-10. [葛书霞, 姚姣, 丁朝霞, 等. 卵巢上皮性癌组织ERβ和ERK1/2表达及其相关性 分析[J] 中华肿瘤防治杂志, 2013, 20(3): 206-10.]
    [10] Jia MQ, Chen ZY, Ling Y, et al. Key protein expression in the insulin-like growth factor-1 signal pathway involved in the resistance of ovarian cancer to cisplatin[J]. Zhongguo Zhong Liu Lin Chuang, 2014, 41(5): 286-90. [贾美群, 陈曾燕, 施玲燕, 等. IGF1信号通路IGF1 IGF1R及AKT在卵巢癌顺铂耐药中表达的 研究[J] 中国肿瘤临床, 2014, 41(5): 286-90.]
    [11] Yong HY, Kim IY, Kim JS, et al. ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling[J]. Int J Oncol, 2010, 36 (2): 501-7.
    [12] Chou RH, Hsieh SC, Yu YL, et al. Fisetin inhibits migration and invasion of human cervical cancer cells by down-regulating urokinase plasminogen activator expression through suppressing the p38 MAPK-dependent NF-κB signaling pathway[J]. PLoS One, 2013, 8(8): e71983.
    [13] Wang R, Li ZQ, Han X, et al. Integrin β3 and its ligand regulate the expression of uPA through p38 MAPK in breast cancer[J]. APMIS, 2010, 118(12): 909-17.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1649
  • HTML全文浏览量:  378
  • PDF下载量:  479
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-08-24
  • 修回日期:  2014-12-26
  • 刊出日期:  2015-08-24

目录

摘要
HTML全文
    参考文献(13)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量：12445209 今日访问：22432

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭