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miR-200c增强肺癌A549细胞对紫杉醇及吉非替尼的敏感度及相关机制

胡丽丽, 尹燕军, 钟文娟, 邱峰

胡丽丽, 尹燕军, 钟文娟, 邱峰. miR-200c增强肺癌A549细胞对紫杉醇及吉非替尼的敏感度及相关机制[J]. 肿瘤防治研究, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003
引用本文: 胡丽丽, 尹燕军, 钟文娟, 邱峰. miR-200c增强肺癌A549细胞对紫杉醇及吉非替尼的敏感度及相关机制[J]. 肿瘤防治研究, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003
HU Lili, YIN Yanjun, ZHONG Wenjuan, QIU Feng. miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003
Citation: HU Lili, YIN Yanjun, ZHONG Wenjuan, QIU Feng. miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003

miR-200c增强肺癌A549细胞对紫杉醇及吉非替尼的敏感度及相关机制

详细信息
    作者简介:

    胡丽丽(1989-),女,硕士,医师,主要从事胸部肿瘤的基础和临床研究

    通讯作者:

    邱峰,E-mail:lukeqiubmu@163.com

  • 中图分类号: R734.2

miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism

  • 摘要: 目的 观察上调肺癌A549细胞中miR-200c的表达对紫杉醇、吉非替尼敏感度及两药联合作用的影响及其作用机制。方法 用miR-200c转染肺癌A549细胞,Real-time PCR检测miR-200c表达,MTT法和流式细胞仪检测转染miR-200c后A549细胞对紫杉醇、吉非替尼单药及两药联合的敏感度和细胞凋亡的影响,Western blot检测TUBB3蛋白、EGFR、Akt、MAPK磷酸化蛋白及总蛋白的表达。结果 上调miR-200c表达可增强A549细胞对紫杉醇及吉非替尼敏感度并促进细胞凋亡,对两药联合无明显作用。Western blot显示转染miR-200c联合紫杉醇可下调A549细胞中TUBB3蛋白表达,联合吉非替尼下调EGFR和Akt磷酸化蛋白表达,与两药联合对EGFR和Akt磷酸化蛋白表达无明显影响。 结论 miR-200c可增强肺癌A549细胞对紫杉醇、吉非替尼的敏感度,可能分别与抑制TUBB3表达、抑制EGFR和Akt磷酸化蛋白表达有关,而对两药联合无协同抗肿瘤作用。

     

    Abstract: Objective To observe the influence of up-regulating miR-200c expression on the sensitivity of lung cancer cells A549 to paclitaxel, gefitinib and the combination of two drugs and related mechanism. Methods miR-200c was transfected into lung cancer cells A549 using Lipofectamine 2000. The expression of miR-200c was detected by Real-time PCR. Drug sensitivity and cell apoptosis were analyzed with MTT assay and flow cytometry. Western blot was used to detect the expression of TUBB3 protein, EGFR, Akt, MAPK expression of phosphorylated proteins and total proteins. Results The sensitivity of A549 cells to paclitaxel and gefitinib and cell apoptosis were increased after up-regulation of miR-200c expression, but there was no obvious effect to the combination of two drugs. Western blot showed that miR-200c combined with paclitaxel inhibited the expression of TUBB3 protein; miR-200c combined with gefitinib inhibited the expression of EGFR, Akt phosphorylation protein, but there was no obvious difference when miR-200c combined with the combination of two drugs. Conclusion miR-200c could increase the sensitivity of lung cancer cells A549 to paclitaxel and gefitinib, which maybe respectively related to the inhibition of TUBB3 and EGFR, Akt phosphorylation protein, while there is no synergistic antitumor effect on the combination of two drugs.

     

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出版历程
  • 收稿日期:  2014-10-13
  • 修回日期:  2015-01-27
  • 刊出日期:  2015-08-24

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