高级搜索

乳腺浸润性导管癌组织中ALDH1+/CD133+干细胞样细胞与血管生成的关系

宋一民, 王英姿, 吕新全, 薛金慧, 李惠翔

宋一民, 王英姿, 吕新全, 薛金慧, 李惠翔. 乳腺浸润性导管癌组织中ALDH1+/CD133+干细胞样细胞与血管生成的关系[J]. 肿瘤防治研究, 2015, 42(07): 706-711. DOI: 10.3971/j.issn.1000-8578.2015.07.014
引用本文: 宋一民, 王英姿, 吕新全, 薛金慧, 李惠翔. 乳腺浸润性导管癌组织中ALDH1+/CD133+干细胞样细胞与血管生成的关系[J]. 肿瘤防治研究, 2015, 42(07): 706-711. DOI: 10.3971/j.issn.1000-8578.2015.07.014
SONG Yimin, WANG Yingzi, LV Xinquan, XUE Jinhui, LI Huixiang. Relationship of ALDH1+/CD133+ Stem Cell-like Cells with Angiogenesis in Breast Invasive Ductal Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 706-711. DOI: 10.3971/j.issn.1000-8578.2015.07.014
Citation: SONG Yimin, WANG Yingzi, LV Xinquan, XUE Jinhui, LI Huixiang. Relationship of ALDH1+/CD133+ Stem Cell-like Cells with Angiogenesis in Breast Invasive Ductal Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 706-711. DOI: 10.3971/j.issn.1000-8578.2015.07.014

乳腺浸润性导管癌组织中ALDH1+/CD133+干细胞样细胞与血管生成的关系

详细信息
    作者简介:

    宋一民(1979-),男,本科,实验师,主要从事肿瘤病理的实验技术工作

    通讯作者:

    李惠翔,E-mail:lihuixiang19@126.com

  • 中图分类号: R737.9

Relationship of ALDH1+/CD133+ Stem Cell-like Cells with Angiogenesis in Breast Invasive Ductal Carcinoma Tissues

  • 摘要: 目的 探讨乳腺浸润性导管癌组织中肿瘤干细胞标志物ALDH1、CD133的表达及其与肿瘤血管生成的关系。方法 应用免疫组织化学双染法检测120例乳腺浸润性导管癌组织中ALDH1+/CD133+ 干细胞样细胞,单染法检测血管性标记CD34、CD105及VEGF的表达情况。统计ALPH1+/CD133+干细胞样细胞与临床病理因素;CD34、CD105与VEGF的关系。结果 25.83%(31/120)的病例存在ALDH1+/CD133+干细胞样细胞,ALDH1+/CD133+干细胞样细胞与ER、VEGF的表达及MVD均相关(P<0.05),但与年龄、肿瘤直径、PR、Her-2、组织学分级及淋巴结转移均无关(P>0.05)。结论 乳腺浸润性导管癌组织中ALDH1+/CD133+干细胞样细胞可能通过调节VEGF的表达促进肿瘤新生血管的生成。

     

    Abstract: Objective To explore the expression of cancer stem cell markers, ALDH1 and CD133, in breast invasive ductal carcinoma tissues and elucidate their relationship with angiogenesis. Methods The expression levels of ALDH1/CD133, CD34, CD105, and VEGF were detected by immunohistochemical double and single staining methods in 120 specimens of breast invasive ductal carcinoma. Results The expression rate of ALDH1+/CD133+ cells in the cancer tissues was 25.83%(31/120). It was closely associated with microvessel density(MVD), VEGF and ER expression (P<0.05), while no significant association with age, tumor size, PR, Her-2, histological grade or lymph node metastasis(P>0.05). Conclusion In breast invasive ductal carcinoma tissues, ALDH1+/CD133+ stem cells-like cells may promote angiogenesis by improving the expression of VEGF.

     

  • [1] Ginestier C, Hur MH, Charafe-Jauffret E, et al. ALDH1 is a marker of normal and malignant human mammary stem cells and apredictor of poor clinical outcome[J]. Cell Stem Cell, 2007, 1(5): 55 5-67.
    [2] Wang R, Chadalavada K, Wilshire J, et al. Glioblastoma stem-like cells give rise to tumor endothelium[J]. Nature, 2010, 468(7325): 82 9-33.
    [3] Hui YZ. Ackerman’s Surgical Pathology Volume Two[M]. Peking University Medical Press, 2006:1825. Rosai J original. [回允中. 阿 克曼外科病理学下册[M]. 北京大学医学出版社, 2006, 1825. [罗 塞(Rosai.J.) 原著.]
    [4] Shimizu M, Saitoh Y, Itoh H. Immunohistochemical staining of Ha-ras ongene product in normal, benign, and malignant Human pancreatic tissues[J]. Hum Pathol, 1990, 21(6): 607-12.
    [5] Gil J, Stembslska A, Pesz KA, et al. Cancer stem cells: the theory and perspectives in cancer therapy[J]. J Appl Genet, 2008, 49(2): 19 3-9.
    [6] Wright MH, Calcagno AM, Salcido CD, et al. Brcal breast tumors contain distinct CD44+CD24- and CD133+ cells with cancer stem cell characteristics[J]. Breast Cancer Res, 2008, 10(1): R10.
    [7] Yang ZF, Ngai P, Ho DW, et al. Identification of local and circulating cancer stem cells in human liver cancer[J]. Hepatology, 20 08, 47(3): 919-28.
    [8] O’Brien CA, Pollett A, Gallinger S, et al. A human colon cancer cell capable of initiating tumor growth in immunodeficient mice[J]. Nature, 2007, 445(7123): 106-10.
    [9] Moeller BJ, Cao Y, Li CY, et al. Radiation activates HIF-1to regulate vascular radiosensitivity in tumors role of reoxygenation, free radicals, and stress granules[J]. Cancer Cell, 2004, 5(5): 42 9-41.
    [10] Platet N, Liu SY, Atifi ME, et al. Influence of oxygen tension on CD133 phenotype in human glioma cell cultures[J]. Cancer Lett, 20 07, 258(2): 286-90.
    [11] Bao S, Wu Q, Sathornsumetee S, et al. Stem cell-like glioma cells promote tumor angiogenesis through vascular endothelial growth factor[J]. Cancer Res, 2006, 66(16): 7843-8.
    [12] Panchision DM. The role of oxygen in regulating neural stem cells in development and disease[J]. J Cell Physiol, 2009, 220(3): 56 2-8.
    [13] Soeda A, Park M, Lee D, et al. Hypoxia promotes expansion of the CD133-positive glioma stem cells through activation of HIF-1 alpha[J]. Oncogene, 2009, 28(45): 3949-59.
    [14] Ping YF, Yao XH, Jiang JY, et al. The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumor angiogenesis via PI2K/AKT signaling[J]. J Pathol, 2011, 224(3): 344-54.
    [15] Silva IA, Bai S, McLean K, et al. Aldehyde dehydrogenase in combination with CD133 defines angiogenic ovarian cancer stem cells that portend poor patient survival[J]. Cancer Res, 2011, 71 (11): 3991-4001.
    [16] Morimoto K, Kin SJ, Tanei T, et al. Stem cell marker aldechyde dehydroenase 1-positive breast cancers are characterized by negative estrogen receptor, positive human epidermal growth factor receptor type 2, and high Ki67 expression[J]. Cancer Sci, 20 09, 100(6): 1062-8.
    [17] Liu XB, Yuan ZY, Li RY, et al. Analysis of the clinicopathologic characteristics and prognosis of breast cancer patients with ER or PR single-positive status[J]. Zhongguo Zhong Liu Lin Chuang, 20 10, 37(7): 381-4. [刘晓斌, 袁智勇, 李瑞英, 等. ER PR单阳性 表达乳腺癌患者临床病理特征和预后分析[J]. 中国肿瘤临床, 20 10, 37(7): 381-4.]
    [18] Fanelli M, Locopo N, Gattuso D, et al. Assessment of tumor vaseularlzation: immunohistochemieal and non-invasive methods[J]. Int J Biol Markers, 1999, 14(4): 218-31.
    [19] Pufe T, Harde V, Petersen W, et al. Vascular endothelial growth factor(VEGF) induces matrix metalloproteinase in immortalized chondrocytes[J]. J Pathol, 2004, 202(3): 367-74.
    [20] Takase Y, Kai K, Masuda M, et al. Endoglin (CD105) expression and angiogenesis status in small cell lung cancer[J]. Pathol Res Pract, 2010, 206(11): 725-30.
计量
  • 文章访问数:  1164
  • HTML全文浏览量:  291
  • PDF下载量:  543
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-08-03
  • 修回日期:  2015-02-27
  • 刊出日期:  2015-07-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭