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胃癌组织中间隙连接超微结构改变及间隙连接蛋白-43的表达及其意义

李春辉, 潘理会, 徐贝贝, 刘海旺, 程玉

李春辉, 潘理会, 徐贝贝, 刘海旺, 程玉. 胃癌组织中间隙连接超微结构改变及间隙连接蛋白-43的表达及其意义[J]. 肿瘤防治研究, 2015, 42(07): 697-701. DOI: 10.3971/j.issn.1000-8578.2015.07.012
引用本文: 李春辉, 潘理会, 徐贝贝, 刘海旺, 程玉. 胃癌组织中间隙连接超微结构改变及间隙连接蛋白-43的表达及其意义[J]. 肿瘤防治研究, 2015, 42(07): 697-701. DOI: 10.3971/j.issn.1000-8578.2015.07.012
LI Chunhui, PAN Lihui, XUN Beibei, LIU Haiwang, CHENG Yu. Clinical Significance of Gap Junction Ultrastructure Change and Connexin-43 Expression in Gastric Cancer Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 697-701. DOI: 10.3971/j.issn.1000-8578.2015.07.012
Citation: LI Chunhui, PAN Lihui, XUN Beibei, LIU Haiwang, CHENG Yu. Clinical Significance of Gap Junction Ultrastructure Change and Connexin-43 Expression in Gastric Cancer Tissues[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 697-701. DOI: 10.3971/j.issn.1000-8578.2015.07.012

胃癌组织中间隙连接超微结构改变及间隙连接蛋白-43的表达及其意义

基金项目: 2 014年度河北省医学科学研究重点课题(ZL20140103);2014年承德市科学技术研究与发展计划(20142030)
详细信息
    作者简介:

    李春辉(1963-),男,博士,主任医师,主要从事胃癌的基础与临床研究

  • 中图分类号: R735.2

Clinical Significance of Gap Junction Ultrastructure Change and Connexin-43 Expression in Gastric Cancer Tissues

  • 摘要: 目的 观察胃癌组织中间隙连接结构的改变,并检测Cx43蛋白及mRNA在组织中的表达,为胃癌的发病机制研究提供新的思路,同时也为药物治疗提供潜在的作用靶点。方法 通过透射电子显微镜技术观察正常胃与胃癌组织中间隙连接的变化;利用Western blot和 RT-PCR技术检测Cx43在正常胃与胃癌组织中的表达水平。结果 胃癌组织超微结构中细胞间隙连接破坏,分化越差破坏越严重,在正常胃组织中Cx43蛋白及mRNA的表达高于胃癌组织中的表达(P<0.05),在胃癌组织中高-中分化者Cx43蛋白及mRNA表达高于低分化者(P<0.05),胃癌组织中TNM分期Ⅰ/ⅡCx43蛋白及mRNA表达高于Ⅲ/Ⅳ期的表达(P<0.05),胃癌组织中浸润深度未侵及浆膜层者Cx43蛋白及mRNA的表达高于侵及浆膜层者(P<0.05),胃癌组织中无淋巴结转移者Cx43蛋白及mRNA的表达高于有淋巴结转移者(P<0.05),Cx43蛋白及mRNA在不同年龄和性别组中的表达差异均无统计学意义(P>0.05)。结论 间隙连接超微结构的改变同Cx43蛋白及mRNA的异常表达与胃癌的发生、发展有关,这为胃癌的发病及靶向治疗提供了新的方向。

     

    Abstract: Objective To observe the changes of gap junctions structure in gastric cancer tissues, and to investigate the protein and mRNA expression of Cx43 in these tissues, then to provide new idea for the research on the mechanism of gastric cancer and new target for the drug treatment. Methods Transmission election microscope(TEM) was used to observe the changes of gap junctions in both normal tissues and gastric cancer tissues. We also used Western blot and PT-PCR technology to detect the expression of Cx43 in normal tissues and gastric cancer tissues. Results The gap junction ultrastructure of gastric cancer tissues had been destructed, and it was more serious with the worse differentiation. The expression of Cx43 protein and mRNA in normal tissues were higher than those in gastric cancer tissues(P<0.05). The expression of Cx43 protein and mRNA in well-moderately differentiated gastric cancer tissues were higher than those in poorly differentiated tissues(P<0.05). The expression of Cx43 protein and mRNA in stage Ⅰ/Ⅱgastric cancer tissues were higher than those in stage Ⅲ/Ⅳ(P<0.05). The expression of Cx43 protein and mRNA in gastric cancer tissues whose depth of invasion couldn't infiltrate serosa were higher than those could infiltrate serosa(P<0.05). The expression of Cx43 protein and mRNA in gastric cancer tissues without lymph node metastasis were higher than those with lymph node metastasis(P<0.05). The expression of Cx43 protein and mRNA had no relationship with age or gender(P>0.05). Conclusion The change of gap junction ultrastructure and the abnormal expression of Cx43 protein and mRNA have relationship with the development of gastric cancer, which provide a new direction for target therapy.

     

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出版历程
  • 收稿日期:  2014-11-30
  • 修回日期:  2015-03-02
  • 刊出日期:  2015-07-24

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