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阿司匹林对人结肠癌细胞株SW480、HT29中VEGF、β-catenin表达的影响

周元昆, 赵逵, 金海, 刘玉娟

周元昆, 赵逵, 金海, 刘玉娟. 阿司匹林对人结肠癌细胞株SW480、HT29中VEGF、β-catenin表达的影响[J]. 肿瘤防治研究, 2015, 42(07): 671-675. DOI: 10.3971/j.issn.1000-8578.2015.07.007
引用本文: 周元昆, 赵逵, 金海, 刘玉娟. 阿司匹林对人结肠癌细胞株SW480、HT29中VEGF、β-catenin表达的影响[J]. 肿瘤防治研究, 2015, 42(07): 671-675. DOI: 10.3971/j.issn.1000-8578.2015.07.007
ZHOU Yuankun, ZHAO Kui, JIN Hai, LIU Yujuan. Effects of Aspirin on VEGF, β-catenin Expression in Colon Cancer Cell Lines SW480 and HT-29[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 671-675. DOI: 10.3971/j.issn.1000-8578.2015.07.007
Citation: ZHOU Yuankun, ZHAO Kui, JIN Hai, LIU Yujuan. Effects of Aspirin on VEGF, β-catenin Expression in Colon Cancer Cell Lines SW480 and HT-29[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 671-675. DOI: 10.3971/j.issn.1000-8578.2015.07.007

阿司匹林对人结肠癌细胞株SW480、HT29中VEGF、β-catenin表达的影响

详细信息
    作者简介:

    周元昆(1980-), 男,硕士,主治医师, 主要从事胃肠道肿瘤的临床工作和研究

    通讯作者:

    赵逵,E-mail: kuizhao95868@msn.com

  • 中图分类号: R735.3+5

Effects of Aspirin on VEGF, β-catenin Expression in Colon Cancer Cell Lines SW480 and HT-29

  • 摘要: 目的 探讨阿司匹林对人结肠癌细胞株HT-29、SW480生长增殖的影响及其β-catenin、VEGF蛋白和mRNA的表达,并为结直肠癌的预防和治疗提供新的实验和理论依据。方法 采用MTT法检测阿司匹林对SW480、HT-29细胞生长增殖的影响;Real-time PCR和Western blot法检测HT-29、SW480细胞中VEGF、β-catenin mRNA及蛋白的表达情况。结果 在SW480、HT-29细胞的生长增殖过程中,阿司匹林对其有着显著的抑制作用,且具有量效、时效关系;除6 h干预组外,余各组阿司匹林均可抑制VEGF、β-catenin蛋白和mRNA的表达(P<0.05)。结论 阿司匹林对人结直肠癌细胞株SW480、HT-29的生长增殖具有显著的抑制作用,并对SW480、HT-29中VEGF、β-catenin mRNA和蛋白质的表达具有显著的下调效应;而阿司匹林通过抑制β-catenin的表达,同时调节VEGF抑制肿瘤血管的生成可能是其预防和治疗结直肠癌的作用机制之一。

     

    Abstract: Objective To investigate the effects of aspirin on the growth and proliferation of human colon cancer cell lines HT-29 and SW480, and the protein and mRNA expression of vascular endothelial growth factor(VEGF) and β-catenin in those cell lines, in order to provide the experimental and theoretical basis for clinical prevention and treatment of colon cancer. Methods We applied aspirin on cultured human colon cancer cell lines HT-29 and SW480, MTT colorimetric assay was used to detect the growth and proliferation of HT-29 and SW480 cell lines. Real-time PCR and Western blot were applied to detect the mRNA and protein expression of VEGF and β-catenin. Results Aspirin had significant inhibitory effects on the growth and proliferation of HT-29 and SW480 cell lines in a dose- and time- dependent manner. Compared with the control group, except 6h intervention group, the mRNA and protein expression of VEGF and β-catenin in other intervention groups were significantly reduced in cultured HT-29 and SW480 cell lines (P<0.05). Conclusion Aspirin has significant inhibitory effects on the growth and proliferation of human colon cancer cell lines HT-29 and SW480, and could reduce the expression of VEGF and β-catenin mRNA and protein. Aspirin inhibiting the expression of β-catenin and VEGF, thereby inhibiting the angiogenesis may be one of the prevention and treatment mechanism of colon cancer.

     

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出版历程
  • 收稿日期:  2014-09-18
  • 修回日期:  2014-12-03
  • 刊出日期:  2015-07-24

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