高级搜索

雪灵芝提取物对H22肝癌荷瘤小鼠免疫功能的影响

杨歆睿, 王刚, 吉立宾, 王海燕

杨歆睿, 王刚, 吉立宾, 王海燕. 雪灵芝提取物对H22肝癌荷瘤小鼠免疫功能的影响[J]. 肿瘤防治研究, 2015, 42(07): 662-665. DOI: 10.3971/j.issn.1000-8578.2015.07.005
引用本文: 杨歆睿, 王刚, 吉立宾, 王海燕. 雪灵芝提取物对H22肝癌荷瘤小鼠免疫功能的影响[J]. 肿瘤防治研究, 2015, 42(07): 662-665. DOI: 10.3971/j.issn.1000-8578.2015.07.005
shu
YANG Xinrui, WANG Gang, JI Libin, WANG Haiyan. Effects of Arenaria Kansuensis Aqueous Extract on Immune Function of H22 Tumorbearing Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 662-665. DOI: 10.3971/j.issn.1000-8578.2015.07.005
Citation: YANG Xinrui, WANG Gang, JI Libin, WANG Haiyan. Effects of Arenaria Kansuensis Aqueous Extract on Immune Function of H22 Tumorbearing Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(07): 662-665. DOI: 10.3971/j.issn.1000-8578.2015.07.005
shu

雪灵芝提取物对H22肝癌荷瘤小鼠免疫功能的影响

  • 810001 西宁，青海大学医学院病理学教研室

基金项目: 国家自然科学基金地区基金（81060369）；青海省应用基础研究项目（2010-Z-722）
详细信息
    作者简介:

    杨歆睿（1986-），女，硕士在读，主要从事肿瘤病理学研究

    通讯作者:

    王刚，E-mail: wanggangqh@163.com

  • 中图分类号: R735.7

Effects of Arenaria Kansuensis Aqueous Extract on Immune Function of H22 Tumorbearing Mice

  • Department of Pathology, Medical College of Qinghai University, Xi'ning 810001, China

摘要

    摘要
  • 摘要: 目的 探讨雪灵芝水提物（arenaria kansuensis aqueous extract, AKAE）对H22荷瘤小鼠免疫功能的影响。方法 建立小鼠肝癌H22实体瘤模型，将小鼠随机分为5组：对照组、环磷酰胺组、AKAE高、中、低剂量组，各组连续给药10 d。断头处死小鼠，称重胸腺和脾脏并计算胸腺、脾脏指数；MTT法检测荷瘤小鼠脾淋巴细胞增殖活性及CTL细胞杀伤活性；采用双抗体夹心ELISA法检测H22荷瘤小鼠血清中IL-2、IFN-γ水平。结果 AKAE处理各组小鼠后，AKAE高、中、低剂量组胸腺指数分别为（21.39±14.3）mg/g、（17.52±7.7）mg/g、（30.57±10.2）mg/g；脾脏指数分别为（38.34±12.7）mg/g、（50.38±29.2）mg/g、（61.63±35.2）mg/g，各剂量组与环磷酰胺组比较具有显著差异，AKAE低剂量组与对照组比较差异具有统计学意义（P＝0.013）；AKAE高剂量组脾淋巴细胞增殖刺激指数（SI）与AKAE低剂量组比较差异具有统计学意义（P＝0.019），而各组CTL杀伤活性无明显差异；AKAE高剂量组IL-2水平较对照组有所升高，而AKAE低剂量组IFN-γ水平明显升高（P＝0.015）。结论 AKAE可以促进H22荷瘤小鼠脾淋巴细胞增殖及Th1型细胞因子的表达。

     

    Abstract: Objective To investigate the effects of Arenaria Kansuensis aqueous extract(AKAE) on the immune function of H22 tumor-bearing mice. Methods Mice bearing H22 solid tumor were randomly divided into five groups: control group, cyclophosphamide(CTX) treatment group and three AKAE treatment groups (high, middle and low dosage). Each group was taken drug through administration by gavage for 10 days. After the mice were killed, the thymus and spleen were weighted respectively to calculate thymus and spleen indexes. The lymphocyte proliferation activity and CTL toxicity were detected by MTT method. The levels of IL-2 and IFN-γ in H22 tumor-bearing mice serum were detected by ELISA. Results After AKAE treatment, the thymus index were (21.39±14.3)mg/g, (17.52±7.7)mg/g, and (30.57±10.2)mg/g in the high, middle and low dosage AKAE group; the spleen index were (38.34±12.7)mg/g, (50.38±29.2)mg/g, and (61.63±35.2)mg/g, respectively. There was significant difference between CTX treatment groups and AKAE treatment groups. There was statistical difference between the low-dose AKAE group and control group(P=0.013). There was statistical difference in the stimulation index(SI) of spleen lymphocyte between high and low-dose AKAE groups(P=0.019). While, there was no significant difference in CTL killing activity among all groups. ELISA results showed that IL-2 expression in the high-dose AKAE group was higher than that in the control group, and IFN-γ expression in low-dose AKAE group was increased obviously compared with the control group(P=0.015). Conclusion AKAE could enhance the proliferation of spleen lymphocytes and promote Th1 cytokines expression in H22 tumor-bearing mice.

     

    • HTML全文
    • 参考文献(12)
  • [1] Jiang SH, Zhou YQ. Mechanism and experimental study of chinese medicine prevention of liver cancer[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2003, 9(4): 69-71. [蒋时红, 周宜强. 中医药 防治肝癌机理及实验研究[J]. 中国中医基础医学杂志, 2003, 9( 4): 69-71.]
    [2] Fu X, Chen W, Duan XQ, et al. Free radical scavenging and antilipoperoxidant properties of Arenaria kansuensis extracts[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2010, 17(7): 35-6. [付翔, 陈薇, 段小群, 等. 雪灵芝提取物清除羟自由基和抑制脂质过氧 化作用[J]. 中国中医药信息杂志, 2010, 17(7): 35-6.]
    [3] Yao SY, Zhao P, Liu RZ, et al. Experiment study on effect of Tibetan A. kansuensis facilitating digestion[J]. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 2007, 23(3): 332-3, 364. [姚思宇, 赵 鹏, 刘荣珍, 等. 西藏雪灵芝促进消化作用实验研究[J]. 中国应 用生理学杂志, 2007, 23(3): 332-3, 364.]
    [4] Zhao P, Yao SY, Wang YW, et al. Effect of A. kansuensis on inhibiting liver cancer induced by diethylnitrosamine in rats[J]. Zhongguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu, 20 07, 11(38): 7553-5. [赵鹏, 姚思宇, 王彦武, 等. 雪灵芝对二乙 基亚硝胺诱导大鼠肝癌组织细胞的抑制作用[J].中国组织工程 研究与临床康复, 2007, 11(38): 7553-5.]
    [5] Wang G, Zhang H, Yang XR, et al. Effects of arenaria kansuensis aqueous extract on proliferation and cell cycle of human gastric cancer cell line MGC-803[J]. Zhong Liu Fang Zhi Yan Jiu, 2013, 40 (9): 821-5. [王刚, 张华, 杨歆睿, 等. 雪灵芝水提物对人胃癌 MGC-803细胞增殖及细胞周期的影响[J]. 肿瘤防治研究, 2013, 40 (9): 821-5.]
    [6] Rao ZL, Zheng JL, Huang W, et al. Immune function indicators and effect of cyclophosphamide on H22 solid tumor-bearing mice[J]. Shi Yan Dong Wu Ke Xue, 2013, 30(3): 10-5, 18, back insert 2. [饶子亮, 郑佳琳, 黄威, 等. H22实体瘤小鼠的免 疫功能指标及环磷酰胺的影响[J]. 实验动物科学, 2013, 30(3): 10 -5, 18, 后插页2.]
    [7] Hu WJ, Liu BR, Qian XP, et al. Effects of chonglou fufang(CLFF) on anti-tumor and immune regulation action in bearing H22 neoplasm mice[J]. Xian Dai Zhong Liu Yi Xue, 2011, 19(11): 21 74-8. [胡文静, 刘宝瑞, 钱晓萍, 等. 重楼复方对荷H22小鼠抑 瘤及免疫功能的影响[J]. 现代肿瘤医学, 2011, 19(11): 2174-8.]
    [8] Wei J, Chen X, Li Q, et al. ELR-CXC chemokine antagonism and cisplatin co-treatment additively reduce H22 hepatoma tumor progression and ameliorate cisplatin-induced nephrotoxicity[J]. Oncol Rep, 2014, 31(4): 1599-604.
    [9] Wang G, Wang HY, Zhang H, et al. Effects of arenaria kansuensis aqueous extract on lymphocytes proliferation and Th1 cytokine production[J]. Zhongguo Mian Yi Xue Za Zhi, 2013, 29(9): 90 5-9. [王刚, 王海燕, 张华, 等. 雪灵芝水提物对淋巴细胞增殖 及Th1型细胞因子表达的影响[J]. 中国免疫学杂志, 2013, 29(9): 90 5-9.]
    [10] Lei N, Du SS, Li L, et al. Chemical constituents from a Tibetan medicine Arenariakansuensis Ⅰ[J]. Zhongguo Zhong Yao Za Zhi, 20 07, 32(10): 918-20. [雷宁, 杜树山, 李林, 等. 藏药甘肃蚤缀的 化学成分研究Ⅰ[J]. 中国中药杂志, 2007, 32(10): 918-20.]
    [11] Liu XX, Wan ZJ, Deng XB, et al. In vitro bioactivity of different extracts from arenaria kansuensis maxim[J]. Ying Yong Yu Huan Jing Sheng Wu Xue Bao, 2010, 16(5): 642-5. [刘笑笑, 万振江, 邓小波, 等. 雪灵芝不同提取物的生物活性[J]. 应用与环境生物 学报, 2010, 16(5): 642-5.]
    [12] Li FW, Zhao P, Liu RZ, et al. Experimental study of arenaria kansuensis regulate immune function in mice[J].Zhongguo Re Dai Yi Xue, 2007, 7(11): 1999-2000, 2020. [李凤文, 赵鹏, 刘荣珍, 等. 雪灵芝调节小鼠免疫作用的实验研究[J]. 中国热带医学, 20 07, 7(11): 1999-2000, 2020.]
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1278
  • HTML全文浏览量:  343
  • PDF下载量:  466
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-12-15
  • 修回日期:  2015-01-21
  • 刊出日期:  2015-07-24

目录

摘要
HTML全文
    参考文献(12)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量：11971810 今日访问：8113

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭