Abstract: Objective To investigate the role of high-risk human papilloma virus(HR-HPV) positive infection in evaluating the cervical lesions in general population, as well as the value of HPV infection in early cervical cancer screening. Methods The women aged 20-60 years-old without cervical cancer screening within one year in Gongshu District, Hangzhou City were detected by Cervista HR-HPV testing in 2013, and HR-HPV positive patients further undergone TCT examination and colposcopy. Results In 879 female residents with HR-HPV positive, 234(26.6%) cases were A5/6 positive, 119(13.5%) cases were A7 positive, 336(38.2%) cases were A9 positive. The most common type of HPV was A9 type in all age groups, the proportions of A9 HR-HPV in 20-30, ≥30-40, ≥40-50 and ≥50 age groups were 35.2%, 42.2%, 37.6% and 36.7% respectively. In 879 female residents with HR-HPV positive, 813(92.5%) residents were found with normal TCT, 66(7.5%) residents were with abnormal TCT. TCT abnormal rates were 6.8%, 5.9% and 9.8% in A5/6, A7 and A9 HPV groups, respectively. Conclusion Positive HR-HPV is not equal as cervical cancer or precancerous lesions. The person with positive A9 HR-HPV should be closely followed up. Whether Cervista HPV-DNA detection could replace cervical cytology, to be a new generation of routine screening method of cervical lesions, needs to accumulate more clinical research data to confirm the feasibility.