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硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究

毛华杰, 朱燕, 卢淑华, 李诚城, 江昊宇, 黄玉梅, 章迪, 王晓春

毛华杰, 朱燕, 卢淑华, 李诚城, 江昊宇, 黄玉梅, 章迪, 王晓春. 硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究[J]. 肿瘤防治研究, 2015, 42(06): 582-586. DOI: 10.3971/j.issn.1000-8578.2015.06.011
引用本文: 毛华杰, 朱燕, 卢淑华, 李诚城, 江昊宇, 黄玉梅, 章迪, 王晓春. 硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究[J]. 肿瘤防治研究, 2015, 42(06): 582-586. DOI: 10.3971/j.issn.1000-8578.2015.06.011
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MAO Huajie, ZHU Yan, LU Shuhua, LI Chengcheng, JIANG Haoyu, HUANG Yumei, ZHANG Di, WANG Xiaochun. Association of Selenoprotein S G-105A, G-254A Polymorphisms with Genetic Susceptibility to Gastric Cancer in Hu'nan Han Population[J]. Cancer Research on Prevention and Treatment, 2015, 42(06): 582-586. DOI: 10.3971/j.issn.1000-8578.2015.06.011
Citation: MAO Huajie, ZHU Yan, LU Shuhua, LI Chengcheng, JIANG Haoyu, HUANG Yumei, ZHANG Di, WANG Xiaochun. Association of Selenoprotein S G-105A, G-254A Polymorphisms with Genetic Susceptibility to Gastric Cancer in Hu'nan Han Population[J]. Cancer Research on Prevention and Treatment, 2015, 42(06): 582-586. DOI: 10.3971/j.issn.1000-8578.2015.06.011
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硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究

  • 1. 410013 长沙，中南大学湘雅医学院医学检验系；2. 410013 长沙，湖南省肿瘤医院检验科；3. 410013 长沙，中南大学湘雅三医院检验科

基金项目: 中南大学研究生自主探索创新项目（2014zzts328）
详细信息
    作者简介:

    毛华杰（1988-），男，硕士在读，主要从事临床生物化学与分子诊断学的研究

    通讯作者:

    王晓春，E-mail: 810768205@qq.com

  • 中图分类号: R735.2；R394.3

Association of Selenoprotein S G-105A, G-254A Polymorphisms with Genetic Susceptibility to Gastric Cancer in Hu'nan Han Population

  • 1. Department of Laboratory Medicine, Xiangya Medical School, Central South University, Changsha 410013, China; 2. Department of Clinical Laboratory Medicine, Hu'nan Provincial of Tumor, Changsha 410013, China; 3. Department of Clinical Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha 410013, China

摘要

    摘要
  • 摘要: 目的 探讨湖南汉族人群硒蛋白S（SelS）基因G-105A、G-254A位点多态性与胃癌遗传易感性的相关研究。方法 采用聚合酶链反应—限制性片断长度多态性（PCR-RFLP）方法，检测113例湖南汉族胃癌患者及111例健康对照者基因型和等位基因频率，分析胃癌患者与健康对照者G-105A、G-254A位点等位基因频率及基因型频率的差异。结果 G-105A位点GG、GA和AA基因型在胃癌组和健康对照组间分别为100.0%、0.0%、0.0%和100.0%、0.0%、0.0%；G、A等位基因频率在胃癌组和健康对照组分别为100.0%、0.0%和100.0%、0.0%，胃癌组和健康对照组基因型和等位基因频率分布无差异。G-254A位点CC、CT和TT基因型在胃癌和健康对照组间分别为50.0%、37.2%、12.8%和65.8%、28.8%、5.4%；C、T等位基因频率在胃癌组和健康对照组分别为69.0%、31.0%和80.2 %、19.8%。胃癌组和健康对照组基因型和等位基因频率分布差异有统计学意义（P＜0.05）。湖南汉族人群中携带T等位基因的个体患胃癌的风险是CC基因型个体的1.89倍（OR=1.89,95%CI:1.10～3.23）。结论 SelSG-254A位点多态性与胃癌的发病具有相关性，T等位基因可能是湖南汉族人群胃癌发病的危险因素之一；湖南汉族人群可能无G-105A这一位点的多态性分布。

     

    Abstract: Objective To investigate the association between selenoprotein S(SelS) gene G-105A,G-254A polymorphisms and genetic susceptibility to gastric cancer in Hu'nan Han population. Methods Polymerase chain reaction restriction fragment length of polymorphism (PCR-RFLP) was taken to detect the genotypes and allele frequencies of the SelS in 113 gastric cancer patients in Hu'nan Han population and 111 agematched healthy controls, to analyze the differences of allele and genotype frequencies of G-105A and G-254A. Results Genotype frequencies of GG, GA, AA in SelS G-105A loci were 100.0%, 0.0%, 0.0% in gastric cancer group, and 100.0%, 0.0%, 0.0% in control group respectively. Allele frequencies of G, A were 100.0%, 0.0% in gastric cancer group and 100.0%, 0.0% in control group respectively. There was no difference of genotype and allele frequencies of SelS G-105A polymorphism between gastric cancer group and control group. Genotype frequencies of CC, CT, TT in SelS G-254A were 50.0%, 37.2%, 12.8% in gastric cancer group and 65.8%, 28.8%, 5.4% in control group respectively. Allele frequencies of C, T were 69.0%, 31.0 in gastric cancer group and 80.2%, 19.8% in control group respectively. There were significant differences of genotype and allele frequencies of SelS G-254A polymorphism between gastric cancer group and control group (P<0.05). The relative risk of suffering from gastric cancer in Hu'nan Han population with T allele was 1.89 times of those with CC genotype (OR=1. 89, 95%CI:1.10-3.23). Conclusion SelS G-254A polymorphism is associated with the risk of gastric cancer. T allele may be a risk factor for gastric cancer in Hu'nan Han population. There may be no G-105A polymorphism distribution in Hu'nan Han population.

     

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出版历程
  • 收稿日期:  2014-09-25
  • 修回日期:  2014-12-15
  • 刊出日期:  2015-06-24

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