Clinical Value of DNA Damage Detection of Peripheral Blood Mononuclear Cells in Diagnosis of Multiple Primary Lung Cancer Patients
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摘要: 目的 本研究旨在探讨外周血单个有核细胞DNA损伤在肺多原发癌和转移癌鉴别诊断中的临床应用价值。方法 应用微量全血单细胞凝胶电泳技术（彗星实验）分别检测28例转移癌、30例肺多原发癌和29例原发肺癌患者外周血单个有核细胞DNA损伤率及损伤程度。结果 转移癌组外周血单个有核细胞损伤阳性率为6.29%（176/2800），显著高于肺多原发癌组3.77%（113/3000），（χ2=18.883,P=0.000）；肺多原发癌组显著高于原发肺癌组2.66%（77/2900），（χ2=5.494, P=0.019）。转移癌组外周血单个有核细胞DNA损伤的尾长、尾矩和椭圆矩分别为（44.19±13.48）μm、（4.53±1.66）和（4.64±1.50） ，显著高于肺多原发癌组[（34.85±8.40）μm、（3.30±1.04）和（3.54±1.03） ]，（P=0.000）；肺多原发癌组显著高于原发肺癌组[（26.25±7.64）μm 、（2.20±0.98） 和（2.37±0.78）]，（P=0.000）。结论 检测外周血单个有核细胞DNA损伤有助于肺多原发癌与转移癌的鉴别诊断。Abstract: Objective To discuss the clinical value of DNA damage detection of peripheral blood mononuclear cells in the diagnosis of multiple primary lung cancer and metastatic carcinoma. Methods By micro-whole blood single cell gel electrophoresis assay (comet experiments), we detected DNA damage rate and damage degree of peripheral blood mononuclear cells of 28 patients with pulmonary metastasis carcinoma, 30 patients with multiple primary lung cancer, and 29 patients with primary lung cancer. Results DNA damage rate of peripheral blood mononuclear cell in pulmonary metastatic carcinoma group was 6.29%(176/2800), which was significantly higher than 3.77%(113/3000) in multiple primary lung cancer group(χ2=18.883, P=0.000); moreover, DNA damage rate of peripheral blood mononuclear cell in multiple primary lung cancer group was also significantly higher than 2.66% (77/2900) in primary lung cancer group(χ2= 5.494, P= 0.019). DNA damage tail length, tail moment and olive moment of patients in pulmonary metastatic carcinoma group[(44.19±13.48)μm, (4.53±1.66), (4.64±1.50)] were significantly higher than those in multiple primary lung cancer group[(34.85±8.40)μm,(3.30±1.04) and (3.54±1.03)](P=0.000); moreover,DNA damage tail length, tail moment and olive moment of patients with multiple primary lung cancer group was significantly higher than those in primary lung cancer group[(26.25±7.64)μm, (2.20±0.98) and (2.37±0.78)](P=0.000). Conclusion The detection of DNA damage in peripheral blood mononuclear cells is helpful to diagnose the difference between multiple primary lung cancer and pulmonary metastatic carcinoma.
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Key words:
- DNA damage /
- Multiple primary neoplasm /
- Lung cancer
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[1] Muela Molinero A, Jorquera Plaza F, Ribas Ariño T, et al. Multiple malignant primary neoplasms in patients with gastric neoplasms in the health district of León[J]. Rev Esp Enferm Dig, 2006, 98 (12): 907-16. [2] Mughal A, Vikram A, Ramarao P, et al. Micronucleus and comet assay in the peripheral blood of juvenile rat: establishment of assay feasibility, time of sampling and the induction of DNA damage[J]. Mutat Res, 2010, 700(1-2): 86-94. [3] Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations[J]. Mol Biotechnol, 2004, 26 (3): 249-61. [4] Che GW, Zhou QH. The new concept about surgical treatment of multiple primary lung cancer[J]. Zhonghua Yi Xue Za Zhi, 2007, 87 (23): 1653-5.[车国卫, 周清华. 肺重复癌外科治疗新观念[J]. 中华医学杂志, 2007, 87(23): 1653-5.] [5] Karahalil B, Bohr VA, Wilson DM 3rd. Impact of DNA polymorphisms in key DNA base excision repair proteins on cancer risk[J]. Hum Exp Toxicol, 2012, 31(10): 981-1005. [6] Letkova L, Matakova T, Musak L，et al. DNA repair genes polymorphism and lung cancer risk with the emphasis to sex differences[J]. Mol Biol Rep, 2013, 40(9): 5261-73. [7] Nogueira A, Assis J, Catarino R, et al. DNA repair and cytotoxic drugs: the potential role of RAD51 in clinical outcome of nonsmall- cell lung cancer patients[J]. Harmacogenomics, 2013, 14(6): 68 9-700. [8] Marabini L, Calò R, Braga PC. Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNAdamage in lung epithelial cells[J]. Arzneimittelforschung, 2011, 61 (12): 700-6. [9] Lee SY, Kang HG, Yoo SS, et al. Polymorphisms in DNA repair and apoptosis-related genes and clinical outcomes of patients with non-small cell lung cancer treated with first-line paclitaxelcisplatin chemotherapy[J]. Lung Cancer, 2013, 82(2): 330-9. [10] Orlow I, Park BJ, Mujumdar U, et al. DNA damage and repair capacity in patients with lung cancer: prediction of multiple primary tumors[J]. J Clin Oncol, 2008, 26(21): 3560-6. [11] Szeto YT, Lee AK, Benzie IF, et al. Optimized noninvasive procedures to measure DNA damage in comet assay[J]. Hum Exp Toxicol, 2012, 31(11): 1144-50. [12] Hussain T, Kotnis A, Sarin R, et al. Genetic susceptibility to multiple primary neoplasms in the upper aero-digestive tract: Genotype Score and phenotype correlation[J]. Cancer Lett, 2013, 33 2(1): 46-54.
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