Expression of Specific Long Non-coding RNA in Nasopharyngeal Carcinoma and Related Significance
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摘要: 目的 分析长链非编码RNA（long non-coding RNA, lncRNA）在鼻咽癌与慢性鼻咽炎组织中表达水平的变化，寻找可能与鼻咽癌发生发展相关的特异lncRNA。方法 采用lncRNA芯片技术分析lncRNA和mRNA在鼻咽癌与慢性鼻咽炎组织的表达谱，筛选差异表达的lncRNA和mRNA，进一步研究使用了聚类分析、GO分析、pathway分析等生物信息学技术。结果 差异表达的lncRNA（2倍以上变化且P＜0.05）共856条，其中上调的共 425条，下调的共431条；差异表达的mRNA共767条，其中上调的共426条，下调的共341条。利用聚类分析方法将差异表达的lncRNA分成3类：增强子型lncRNA、HOX 基因簇、基因间lncRNA。GO分析将mRNA分成3类：生物学过程类、细胞组分类、分子功能类。Pathway分析显示mRNA共参与28条信号通路，其中参与细胞因子-细胞因子受体相互作用信号通路的上调差异表达的mRNA的富集度最高。结论 与慢性鼻咽炎相比，lncRNA在鼻咽癌组织的表达水平明显改变，提示差异表达的lncRNA可能与鼻咽癌的发生发展有关。
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关键词:
- 鼻咽癌 /
- 长链非编码RNA /
- 长链非编码RNA芯片
Abstract: Objective To explore specific long non-coding RNA(lncRNA) related to the occurrence and development of nasopharyngeal carcinoma(NPC) by analyzing lncRNA expression profile in NPC and chronic nasopharyngitis(CNP) tissues. Methods To screen the differentially expressed lncRNA and mRNA, lncRNA and mRNA expression profiles between NPC and CNP tissues were analyzed by microarrays. Bioinformatic analyses, such as clustering analysis, gene ontology analysis and pathway analysis, were used for further research. Results A total of 856 lncRNAs were differentially expressed with more than two-fold change (P<0.05), 425 were up-regulated and 431 were down-regulated in NPC tissues. A total of 767 mRNAs were differentially expressed, 426 were up-regulated and 341 were down-regulated. These lncRNAs were divided by clustering analysis method into three sub-groups, enhancer-like lncRNA, HOX cluster and lincRNA. GO analysis was performed to clarify the mRNAs into three kinds, biological process, cellular component and molecular function.Pathway analysis indicated that 28 signaling pathways corresponded to up-regulated and down-regulated mRNA. The enrichment score of up-regulated mRNAs participated in the pathway of cytokine-cytokine receptor interaction was the highest. Conclusion lncRNAs are significantly differentially expressed between NPC and CNP tissues. Specific lncRNA may play a key role in the occurrence and development of NPC. -
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