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腹腔灌注顺铂治疗胃癌恶性腹水的疗效与Beclin-1 mRNA表达变化的关系

赖敏栋, 张慧卿, 陈文学, 何波, 芦珊, 万以叶

赖敏栋, 张慧卿, 陈文学, 何波, 芦珊, 万以叶. 腹腔灌注顺铂治疗胃癌恶性腹水的疗效与Beclin-1 mRNA表达变化的关系[J]. 肿瘤防治研究, 2015, 42(01): 45-47. DOI: 10.3971/j.issn.1000-8578.2015.01.011
引用本文: 赖敏栋, 张慧卿, 陈文学, 何波, 芦珊, 万以叶. 腹腔灌注顺铂治疗胃癌恶性腹水的疗效与Beclin-1 mRNA表达变化的关系[J]. 肿瘤防治研究, 2015, 42(01): 45-47. DOI: 10.3971/j.issn.1000-8578.2015.01.011
LAI Mindong, ZHANG Huiqing, CHEN Wenxue, HE Bo, LU Shan, WAN Yiye. Changes of Beclin-1 mRNA Expression for Predicting Efficacy of Introperitoneal Cisplatin in Treatment of Gastric Cancer with Malignant Ascites[J]. Cancer Research on Prevention and Treatment, 2015, 42(01): 45-47. DOI: 10.3971/j.issn.1000-8578.2015.01.011
Citation: LAI Mindong, ZHANG Huiqing, CHEN Wenxue, HE Bo, LU Shan, WAN Yiye. Changes of Beclin-1 mRNA Expression for Predicting Efficacy of Introperitoneal Cisplatin in Treatment of Gastric Cancer with Malignant Ascites[J]. Cancer Research on Prevention and Treatment, 2015, 42(01): 45-47. DOI: 10.3971/j.issn.1000-8578.2015.01.011

腹腔灌注顺铂治疗胃癌恶性腹水的疗效与Beclin-1 mRNA表达变化的关系

基金项目: 江西省青年科学基金(20122BAB215029);江西省科技计划项目(20133BBG70040)
详细信息
    作者简介:

    赖敏栋(1983-),男,硕士在读,主要从事消化道肿瘤的相关研究

    通讯作者:

    张慧卿,E-mail:hqzhang888@sina.com

  • 中图分类号: R735.2

Changes of Beclin-1 mRNA Expression for Predicting Efficacy of Introperitoneal Cisplatin in Treatment of Gastric Cancer with Malignant Ascites

  • 摘要: 目的 探讨腹水脱落癌细胞Beclin-1 mRNA表达变化对顺铂腹腔化疗治疗胃癌恶性腹水疗效的预测作用。方法 35例胃癌恶性腹水患者,均接受腹腔内灌注顺铂,60毫克/次,1次/周。采用WHO标准评价疗效,根据疗效不同将患者分成治疗有效组(OR)和无效组(NR)两组;收集化疗前和首次腹腔化疗后第5天的腹水各20 ml,分离腹水中脱落癌细胞,RT-PCR技术检测腹水脱落癌细胞Beclin-1和Bcl-2 mRNA的表达。结果 54.3%(19/35)的患者治疗有效,与NR组患者相比,OR组患者以Beclin-1和Bcl-2 mRNA降低为主(均P<0.05);统计分析显示,Beclin-1 mRNA下降预测化疗有效的敏感度达86.7%,特异性为70.0%。结论 监测腹水脱落癌细胞中Beclin-1 mRNA水平的变化,有助于预测腹腔内灌注顺铂治疗胃癌恶性腹水的近期疗效。

     

    Abstract: Objective To investigate the role of Beclin-1 mRNA expression changes in exfoliative cancer cells in predicting the efficacy of introperitoneal cisplatin chemotherapy in gastric cancer patients with malignant ascites. Methods A total of 35 gastric cancer patients with malignant ascites received the treatment of introperitoneal cisplatin (60 mg, Q1W). WHO criteria was employed to evaluate the objective response of chemotherapy, and then patients were divided into objective response (OR) group and no response (NR) group. 20 ml ascties were collected before chemotherapy and 5 days after chemotherapy, respectively; then exfoliative cancer cells were isolated. Beclin-1 and Bcl-2 mRNA expression were detected by RT-PCR. Results There were 54.3% (19/35) cases acquired objective response, mainly with decreased Beclin-1 and Bcl-2 mRNA expression, compared with NR group (P<0.05). Sensitivity and specificity of decreased Beclin-1 mRNA expression in predicting treatment efficacy were 86.7% and 70.0%, respectively. Conclusion Monitoring the changes of Beclin-1 mRNA expression in exfoliative cancer cells may be effective for predicting the short-term effects of introperitoneal cisplatin on gastric cancer patients with malignant ascites.

     

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出版历程
  • 收稿日期:  2014-02-17
  • 修回日期:  2014-03-03
  • 刊出日期:  2015-01-24

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