高级搜索

卵巢癌SKOV3细胞中侧群细胞的生物学特性

钟艳平, 孟泳圳, 李力, 尹富強, 黎丹戎, 张玮

钟艳平, 孟泳圳, 李力, 尹富強, 黎丹戎, 张玮. 卵巢癌SKOV3细胞中侧群细胞的生物学特性[J]. 肿瘤防治研究, 2014, 41(12): 1282-1285. DOI: 10.3971/j.issn.1000-8578.2014.12.006
引用本文: 钟艳平, 孟泳圳, 李力, 尹富強, 黎丹戎, 张玮. 卵巢癌SKOV3细胞中侧群细胞的生物学特性[J]. 肿瘤防治研究, 2014, 41(12): 1282-1285. DOI: 10.3971/j.issn.1000-8578.2014.12.006
shu
ZHONG Yanping, MENG Yongzhen, LI Li, YIN Fuqiang, LI Danrong, ZHANG Wei. Biological Characteristics of Side Population Cells in Ovarian Cancer Cell Line SKOV3[J]. Cancer Research on Prevention and Treatment, 2014, 41(12): 1282-1285. DOI: 10.3971/j.issn.1000-8578.2014.12.006
Citation: ZHONG Yanping, MENG Yongzhen, LI Li, YIN Fuqiang, LI Danrong, ZHANG Wei. Biological Characteristics of Side Population Cells in Ovarian Cancer Cell Line SKOV3[J]. Cancer Research on Prevention and Treatment, 2014, 41(12): 1282-1285. DOI: 10.3971/j.issn.1000-8578.2014.12.006
shu

卵巢癌SKOV3细胞中侧群细胞的生物学特性

  • 1.530021 南宁，广西医科大学医学科学实验中心；2.530021 南宁，广西医科大学肿瘤医院妇瘤科

详细信息
    作者简介:

    钟艳平（1971-），女，硕士，助理研究员，主要从事肿瘤分子标志物研究

  • 中图分类号: R737.31

Biological Characteristics of Side Population Cells in Ovarian Cancer Cell Line SKOV3

  • 1. Medical and Scientific Center of Guangxi Medical University, Nanning 530021, China;2.Department of Gynecological Oncology, Tumor Hospital of Guangxi Medical University, Nanning 530021, China

摘要

    摘要
  • 摘要: 目的 分选人卵巢癌细胞系SKOV3中的侧群（side population, SP）细胞，并探讨其是否具有肿瘤干细胞的生物学特性。方法 流式细胞仪检测、分选经DNA染料Hoechst 33342染色后SKOV3中的SP细胞，并对侧群细胞（SP）与非侧群细胞（non-SP）作细胞生物学鉴定，包括增殖能力、克隆形成能力、侵袭及迁移能力、自我更新能力及细胞周期。结果 SKOV3细胞系中SP细胞比例为（1.12± 0.104）%，SP细胞增殖速度快于non-SP细胞，差异有统计学意义（P<0.05）。接种相同数量的细胞，SP细胞克隆形成率高于non-SP细胞（P<0.05）。Transwell实验显示，SP细胞侵袭与迁移的细胞数与non-SP相比均明显增多（P<0.05）。SP细胞体外能分化为Non-SP细胞，具有自我更新能力。SP细胞大多处于细胞周期的G0/G1期。结论 卵巢癌细胞系SKOV3中的SP细胞具有肿瘤干细胞的生物学特性，SP细胞表型可考虑作为富集卵巢癌干细胞样细胞的有效方法。

     

    Abstract: Objective To identify the existence of side population(SP) cells in human ovarian cancer cell line SKOV3 and to investigate whether SP cells have the biological characteristics of cancer stem cells. Methods SP and non-SP cells from SKOV3 were isolated by fluorescence-activated cell sorting after being stained by DNA-binding dye Hoechst 33342. Biological characteristics of SP and non-SP cells were evaluated, such as proliferation ability, colony-forming efficiency, invasion and migration ability, self-renewal ability and cell cycle. Results SP cells proportion in SKOV3 cell line was (1.12±0.104)%. The proliferation ability of SP cells was obviously greater than that of non-SP cells(P<0.05). The colony-forming efficiency of SP cells was also higher than that of non-SP cells(P<0.05). SP cells had a stronger ability of invasion and migration compared with non-SP cells(P<0.05). SP cells could differentiate into non-SP cells in vitro. Most of SP cells were in the G0/G1 phase. Conclusion SP cells in SKOV3 exhibit biological characteristics of cancer stem cells. The phenotype of SP cells could be used as a potentially useful tool for enriching ovarian cancer stem cells.

     

    • HTML全文
    • 参考文献(11)
  • [1] Wu C, Alman BA. Side population cells in human cancers[J]. Cancer Lett, 2008, 268(1): 1-9.
    [2] Hu H, Wang WH, Jiao F, et al. Update of pancreatic cancer stem cell research[J]. Zhong Liu Fang Zhi Yan Jiu, 2013, 40(8): 80 1-4.[胡海, 汪卫华, 焦锋, 等. 胰腺癌干细胞研究进展[J]. 肿瘤 防治研究, 2013, 40(8): 801-4.]
    [3] Mueller MT, Hermann PC, Witthauer J, et al. Combined target treatment to eliminate tumorigenic cancr stem cells in human pancreatic cancer[J]. Gastroenterology, 2009,137(3): 1102-13.
    [4] Fong MY, Kakar SS. The role of cancer stem cells and the side population in epithelial ovarian cancer[J]. Histol Histopathol, 20 10, 25(1): 113-20.
    [5] Moserle L, Indraccolo S, Ghisi M, et al. The side population of ovarian cancer cells is a primary target of IFN-alpha antitumor effects[J]. Cancer Res, 2008, 68(14): 5658-68.
    [6] Meng YZ, Li L, Zhong YP, et al. Establishment and discussion of a method for sorting ovarian cancer side population through flow cytometry[J].Guangxi Yi Ke Da Xue Xue Bao,2013,30(2):175-80 [孟泳圳, 李力, 钟艳平, 等. 卵巢癌侧群细胞流式分选方法的建 立及探讨[J]. 广西医科大学学报, 2013, 30(2): 175-80.]
    [7] Wang J, Guo LP, Chen LZ, et al. Identification of cancer stem cell- like side population cells in human nasopharyngeal carcinoma cell line[J]. Cancer Res, 2007, 67(8): 3716-24.
    [8] Chiba T, Kita K, Zheng YW, et al. Side population purified from hepatocellular carcinoma cells harbors cancer stem cell-like properties[J]. Hepatology, 2006, 44(1): 240-51.
    [9] Mitsutake N, Iwao A, Nagai K, et al. Characterization of side population in thyroid cancer cell lines: cancer stem-like cells are enriched partly but not exclusively[J]. Endocrinology, 2007, 14 8(4): 1797-803.
    [10] Horsley V, Aliprantis AO, Polak L, et al. NFATc1 balances quiescence and proliferation of skin stem cells[J]. Cell, 2008, 13 2(2): 299-310.
    [11] Kondo T, Setoguchi T, Taga T. Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line[J]. Proc Natl Acad Sci U S A, 2004, 101(3): 781-6.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1478
  • HTML全文浏览量:  281
  • PDF下载量:  811
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-12-15
  • 修回日期:  2014-04-10
  • 刊出日期:  2014-12-24

目录

摘要
HTML全文
    参考文献(11)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量： 今日访问：

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭