高级搜索

益气除痰方对A549细胞增殖抑制并诱导凋亡的研究

宋妮, 周京旭, 林丽珠

宋妮, 周京旭, 林丽珠. 益气除痰方对A549细胞增殖抑制并诱导凋亡的研究[J]. 肿瘤防治研究, 2014, 41(11): 1200-1204. DOI: 10.3971/j.issn.1000-8578.2014.11.009
引用本文: 宋妮, 周京旭, 林丽珠. 益气除痰方对A549细胞增殖抑制并诱导凋亡的研究[J]. 肿瘤防治研究, 2014, 41(11): 1200-1204. DOI: 10.3971/j.issn.1000-8578.2014.11.009
SONG Ni, ZHOU Jingxu, LIN Lizhu. Yiqi Chutan Decoction Inhibits Proliferation and Induces Apoptosis of A549 Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(11): 1200-1204. DOI: 10.3971/j.issn.1000-8578.2014.11.009
Citation: SONG Ni, ZHOU Jingxu, LIN Lizhu. Yiqi Chutan Decoction Inhibits Proliferation and Induces Apoptosis of A549 Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(11): 1200-1204. DOI: 10.3971/j.issn.1000-8578.2014.11.009

益气除痰方对A549细胞增殖抑制并诱导凋亡的研究

基金项目: 广东省自然科学基金资助项目(K2070048)
详细信息
    作者简介:

    宋妮(1984-),女,硕士,主治医师,主要从事中西医结合治疗肿瘤的基础及临床研究

    通讯作者:

    林丽珠,E-mail:lizhulin903@21cn.com

  • 中图分类号: R734.2

Yiqi Chutan Decoction Inhibits Proliferation and Induces Apoptosis of A549 Cells

  • 摘要: 目的 研究益气除痰方含药血清对肺腺癌A549细胞的增殖抑制作用和诱导凋亡的影响。方法 益气除痰方汤剂给大鼠灌胃,制备含药血清;通过MTT比色法观察A549细胞增殖,并确定血清添加量;通过琼脂糖凝胶电泳和流式细胞仪检测A549细胞凋亡。结果 益气除痰方含药血清对A549 细胞活性具有抑制性,且有时间和浓度依赖性,与空白组比较差异有统计学意义(P<0.05),最终确定10%、20%、30%为实验浓度;琼脂糖凝胶电泳中高剂量组及阳性组可见明显凋亡DNA Ladder 条带,而阴性组、低剂量组、中剂量组均未明显特征性凋亡条带出现;流式细胞仪检测结果提示中低、中、高剂量组、阳性组中A549细胞凋亡率分别为(8.40±1.67)%、(15.80±2.95)%、(27.4± 0.96)%、(39.00±1.11)%,均高于阴性组,与阴性组比较差异有统计学意义(P<0.01)。结论 益气除痰方含药血清对A549细胞具有明显的抑制增殖和诱导凋亡的作用。

     

    Abstract: Objective To investigate the effect of medicated serum of Yiqi Chutan Decoction on the proliferation and apoptosis of human lung carcinoma cells A549. Methods Rats were given Yiqi Chutan Decoction by gavage to get serum containing herb. The proliferation of A549 cells were detected by MTT assay, and the additive amount of serum was determined. The apoptosis of A549 cells was observed by agarose gel electrophoresis and flow cytometry. Results Medicated serum of Yiqi Chutan Decoction could inhibit the activity of A549 cells in a time and concentration dependent manner, with significant difference compared with the blank group (P<0.05). Final concentrations of serum for the experiment were 10% (low dose), 20% (medium dose), and 30% (high dose). Agarose gel electrophoresis results showed obvious apoptotic DNA Ladder bands were found in medium and high dose groups and positive group, while no obvious apoptotic DNA Ladder bands was found in negative group, low or medium dose group. FCM showed the apoptosis of A549 cells in low, medium, high dose groups and positive group were (8.40 ± 1.67)%, (15.80 ± 2.95)%, (27.4 ± 0.96)% and (39.00 ± 1.11)%, which were all higher than that in negative group (P<0.01). Conclusion Medicated serum of Yiqi Chutan Decoction could inhibit the proliferation and induce the apoptosis of A549 cells.

     

  • [1] Zhou DH, Lin LZ, Zhou YQ, et al. The role of TCM method for invigorating Qi and removing the phlegm in prolongating median survival time in the patient with non-small cell lung cancer[J]. Zhong Yi Za Zhi, 2005, 46(8): 600-2.[周岱翰, 林丽珠, 周宜强, 等. 益气除痰法延长非小细胞肺癌中位生存期的作用[J]. 中医 杂志, 2005, 46(8): 600-2.]
    [2] Chen CM,Li YH. Analyses of traditional Chinese medicine decoction method[J]. Xian Dai Zhong Xi Yi Jie He Za Zhi,2006,15(4): 488-9.[陈春梅, 李勇华. 中药煎服法浅析[J]. 现 代中西医结合杂志, 2006, 15(4): 488-9.]
    [3] Chen Q. Study on the method of traditional Chinese Medicine Pharmacology[M]. Third edition. Beijing:People's Medical Publishing House, 2011: 1262-4.[陈奇. 中药药理研究方法学 [M] 第3版. 北京:人民卫生出版社, 2011: 1262-4.]
    [4] Li ZG, Wang JJ. The thinking on the methodology of serum pharmacology on Chinese materia medica[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2002, 9(2): 5-6.[李振光, 王净净. 关于中药血 清药理学方法的思考[J]. 中国中医药信息杂志, 2002, 9(2): 5-6.]
    [5] Hu XM, Zhang PT, Yang ZY, et al. A study on distribution of traditional Chinese medicine syndromes in patients with advanced non-small cell lung cancer[J]. Zhongguo Zhong Liu,2007,16(1): 51 -3.[ 胡小梅,张培彤,杨宗艳,等. 中晚期非小细胞肺癌患者中 医证型分布规律研究[J]. 中国肿瘤, 2007,16(1): 51-3.]
    [6] Chen T,Chen MH. Statistical analysis of clinical types of primary bronchogenic carcinoma of lung in TCM literature[J]. Shizhen Guo Yi Guo Yao, 2006, 17(5): 854-5.[陈涛,陈茂华. 原发性支 气管肺癌临床证型的文献统计分析[J]. 时珍国医国药, 2006, 17 (5): 854-5.]
    [7] Sun LL, Lin LZ. Theoretical study and clinical application of TCM method for invigorating Qi and removing the phlegm in curing lung cancer[J].Zhong Yi Yan Jiu,2011,24(10): 1-3.[孙玲玲, 林丽珠.益气除痰法治疗肺癌理论探讨及临床应用[J].中医研 究, 2011, 24(10): 1-3.]
    [8] Zhang EX. Effect of clinical study on improving life quality of patients suffering lung cancer of non-small cell carcinoma at advanced stage with treating methodology of nourishing qi dissipating phlegm[J]. Zhong Yi Yao Xue Bao,2006,34(5): 20 -1.[张恩欣. 益气除痰法提高晚期非小细胞肺癌患者生存质 量的临床研究[J]. 中医药学报, 2006, 34(5): 20-1.]
    [9] Zhang EX. Effect of invigorating qi and expelling phlegm method and radiofrequency ablation on living quality of patients with medium and advanced lung cancer[J].Fujian Zhong Yi Yao,2009,40(5):16-7.[张恩欣.益气除痰法结合射频消融对中晚 期肺癌患者生存质量的影响[J].福建中医药, 2009, 40(5): 16-7.]
    [10] Chen XZ,Wang YJ,Tian HQ,et al. The clinical curative effect of TCM method for invigorating Qi and removing the phlegm combining with chemotherapy in curing advanced non small cell lung cancer [J].Zhongguo Yi Yao Zhi Nan, 2012,10(19): 332-3. [陈 学彰,王艳杰,田华琴,等.益气除痰法结合化疗治疗中晚期非小 细胞肺癌的临床疗效观察[J].中国医药指南, 2012,10(19):332-3.]
    [11] Iwama H, Amagaya S, Ogihara Y. Effect of shosaikoto, a Javanese and Chinese herbal medicinal mixture on the mitogenic activity of lipopolysaccharide, a new pharmacological testing method[J]. J Ethnohparmacol, 1987, 21(1): 45-53.
    [12] Zhao WH, Cao YX, Yuan ZF. The methodology exploration of serum pharmacology on Chinese materia medica[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 2002, 13(2): 122-4.[赵万红,曹永 孝,袁泽飞.中药血清药理学的方法学探讨[J].中药新药与临床 药理, 2002, 13(2): 122-4.]
    [13] Zhang HM,Xi e CG,Chen SW. An As s e s sment on the Pharmacological Test of Drug Contained Serum[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2004, 24(8): 741-5.[张红敏, 谢春光, 陈世伟.含药血清体外药理试验的评价[J]. 中国中西医结合杂 志,2004, 24(8): 741-5.]
    [14] Shi C,Wan LG. Detection method of cell proliferation [J]. Shi Yan Yu Jian Yan Yi Xue, 2012, 30(2): 153-5, 168. [石淙,万腊根. 细胞 增殖的检测方法[J]. 实验与检验医学, 2012, 30(2): 153-5, 168.]
计量
  • 文章访问数:  1145
  • HTML全文浏览量:  317
  • PDF下载量:  440
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-08-19
  • 修回日期:  2014-01-23
  • 刊出日期:  2014-11-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭