Effect of Ammonium Molybdate on Cell Proliferation and Cycle of Hepatoma and Colon Cancer
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摘要: 目的 观察钼酸氨对体外培养人肝癌和结肠癌细胞的增殖抑制和诱导凋亡作用。方法 采用不同浓度钼酸氨（0.101、0.202、0.404 mmol·L-1）分别对人肝癌细胞株HepG2和结肠癌细胞株HCT-116作用 24、48、72 h后，利用显微镜观察细胞形态学变化，CCK-8法检测细胞增殖抑制，流式细胞仪分析细胞周期变化。结果 随着钼酸氨浓度的增加和作用时间的延长，其对HepG2细胞和HCT-116细胞的增殖抑制作用和诱导凋亡作用愈加明显，存在着显著的量-效和时-效关系，且低浓度（0.101 mmol·L-1）HepG2细胞受抑制显著于HCT-116细胞，高浓度（0.404 mmol·L-1）则相反，差异有统计学意义(P<0.05)；钼酸氨不仅阻止HCT-116细胞由G1/G0期向S期移行，而且阻止HCT-116细胞由G2/M期向G1/G0期移行,将细胞阻滞在G1/G0期和G2/M期,而对HepG2细胞仅将细胞特异性地阻滞在G1/G0 期。钼酸氨可促进HepG2细胞和HCT-116细胞凋亡，并且细胞调亡和坏死率随钼酸氨浓度增加和作用时间延长而逐渐升高。结论 钼酸氨对结肠癌和肝癌细胞均有增殖抑制、诱导凋亡和改变细胞周期作用，且高浓度钼酸氨对结肠癌细胞作用强于肝癌细胞，钼酸盐具有抗癌活性。Abstract: Objective To observe the effect of ammonium molybdate on inhibiting cell proliferation and inducing cell apoptosis of human hepatoma and colon cancer. Methods After human hepatoma cell line HepG2 and colon cancer cell line HCT-116 were treated respectively with 0.101, 0.202 and 0.404 mmol·L-1 of ammonium molybdate for 24, 48 and 72 h, the morphological change was observed by optical microscope, the proliferation inhibition were detected by CCK-8 method, and the change of cell cycle was detected by flow cytometry. Results With the increased concentration and action time of ammonium molybdate, its effect on inhibiting proliferation and inducing apoptosis of HepG2 cells and HCT-116 cells was significantly enhanced, in a significant dose- and time-dependent manner. And HepG2 proliferation was inhibited more obviously than HCT-116 at low concentration of 0.101 mmol·L-1, while it was opposite at high concentration of 0.404 mmol·L-1, with statistical significance(P<0.05). Ammonium molybdate prevented not only the migration of HCT-116 cells from G1/G0 to S period, but also from G2/M to G1/G0 period. Cell cycle of HCT-116 was retarded in G1/G0 and G2/M periods and that of HepG2 was retarded in G1/G0 period. Ammonium molybdate could induce the apoptosis of HepG2 and HCT-116 cells, and the rates of apoptotic and necrotic cells were increased gradually with the increased concentration of ammonium molybdate and prolonged action time. Conclusion Ammonium molybdate has antitumor activity and could inhibit the proliferation, induce the apoptosis and change the cell cycle of colon cancer and hepatoma cells. And the effect of ammonium molybdate at high concentration on colon cancer cells is greater than that on hepatoma cells.
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Key words:
- Ammonium molybdate /
- Colon cancer /
- Hepatoma /
- Proliferation inhibition /
- Cell cycle /
- Cell apoptosis
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