高级搜索

血浆抗凝血酶Ⅲ、绒毛蛋白1和凝集素含量在维吾尔族妇女宫颈病变进程中的动态变化及意义

郭 霞, 玛依拉·卡米力江, 伊力努尔·沙比提, 刘开江, 阿仙姑·哈斯木, 哈丽丹·热依木, Abulizi·Abudula

郭 霞, 玛依拉·卡米力江, 伊力努尔·沙比提, 刘开江, 阿仙姑·哈斯木, 哈丽丹·热依木, Abulizi·Abudula. 血浆抗凝血酶Ⅲ、绒毛蛋白1和凝集素含量在维吾尔族妇女宫颈病变进程中的动态变化及意义[J]. 肿瘤防治研究, 2014, 41(06): 618-621. DOI: 10.3971/j.issn.1000-8578.2014.06.024
引用本文: 郭 霞, 玛依拉·卡米力江, 伊力努尔·沙比提, 刘开江, 阿仙姑·哈斯木, 哈丽丹·热依木, Abulizi·Abudula. 血浆抗凝血酶Ⅲ、绒毛蛋白1和凝集素含量在维吾尔族妇女宫颈病变进程中的动态变化及意义[J]. 肿瘤防治研究, 2014, 41(06): 618-621. DOI: 10.3971/j.issn.1000-8578.2014.06.024
GUO Xia, Mayila·Kamilijiang, Yilinuer·Shabiti, LIU Kaijiang, Axiangu·Hasimu, Halidan·Reyimu, Abulizi·Abudula. Correlation of Dynamic Changes of Candidate Plasma Proteins Expression with Development of Cervical Carcinogenesis in Uyghur Women[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 618-621. DOI: 10.3971/j.issn.1000-8578.2014.06.024
Citation: GUO Xia, Mayila·Kamilijiang, Yilinuer·Shabiti, LIU Kaijiang, Axiangu·Hasimu, Halidan·Reyimu, Abulizi·Abudula. Correlation of Dynamic Changes of Candidate Plasma Proteins Expression with Development of Cervical Carcinogenesis in Uyghur Women[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 618-621. DOI: 10.3971/j.issn.1000-8578.2014.06.024

血浆抗凝血酶Ⅲ、绒毛蛋白1和凝集素含量在维吾尔族妇女宫颈病变进程中的动态变化及意义

基金项目: 国家自然科学基金资助项目(81260380);新疆维吾尔自治区重点实验室基金项目(XJDX0208-2008-03)
详细信息
    作者简介:

    郭霞(1980-),女,博士,助理研究员,主要从事宫颈癌的发病机制与预警研究

    通讯作者:

    Abulizi·Abudula,E-mail: abulizi_de@126.com

  • 中图分类号: R737.33

Correlation of Dynamic Changes of Candidate Plasma Proteins Expression with Development of Cervical Carcinogenesis in Uyghur Women

More Information
    Corresponding author:

    Abulizi·Abudula,E-mail: abulizi_de@126.com

  • 摘要: :目的 根据前期工作中运用血浆蛋白质组学研究筛选出的一系列血浆候选差异蛋白指标,探索血浆中AT Ⅲ、VIL1和CLU表达含量在维吾尔族妇女宫颈病变进程中动态变化。方法 收集82例新疆地区维吾尔族宫颈病变血浆样本(宫颈炎22例,CINⅡ~Ⅲ20例,宫颈癌早期20例,宫颈癌晚期20例),用酶联免疫法检测血浆中ATⅢ、VIL1和CLU的表达含量,分析其与宫颈病变进程的关系及相关性。结果 ATⅢ的表达含量随着宫颈病变进程逐渐下降,CLU和VIL1的表达含量随着宫颈病变的进程逐渐升高(P<0.05)。以宫颈炎组作为对照组,ATⅢ、VIL1和CLU的表达含量与CINⅡ~Ⅲ组、宫颈癌早期组比较差异均有统计学意义(P<0.05)。ATⅢ、VIL1和CLU表达含量之间的动态变化关联性分析差异无统计学意义。结论 维吾尔族妇女宫颈癌发生发展与ATⅢ、VIL1和CLU的表达含量动态变化密切相关,可以成为宫颈癌早期诊断的血浆候选预警蛋白标志物。

     

    Abstract: Objective To explore the relationships of the dynamic changes of candidate plasma proteins expression with the development of cervical carcinogenesis in Uyghur women based on the previous plasma proteomic research. Methods We collected 82 cases of plasma samples from Uyghur women, 20 cases with cervicitis (CV), 20 cases with cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ, 20 cases with early stage (Ⅰ-Ⅱ) and 20 cases with advanced stage (ⅡB-Ⅳ) cervical squamous cell carcinoma, quantitatively determined the content of plasma proteins ATⅢ, VIL1 and CLU by enzyme linked immunosorbent assay (ELISA) and statistical analysis, and evaluated the specifi city and sensitivity of candidate plasma protein markers. Results With the development of cervicitis to CIN Ⅱ-Ⅲ, early stage or advanced stage cervical cancer, the changes of these plasma proteins altered gradually, with remarkably decrease in the content of ATⅢ, and increase of VIL1 and CLU, with statistical differences (P<0.05). Compared with cervicitis group, there were signifi cant differences of the content of plasma proteins ATⅢ, VIL1 and CLU in CINⅡ-Ⅲ, early stage or advanced stage cervical cancer group (P<0.05). Correlation analysis of these three protein indicators showed no correlation (P>0.05). By the detection of ATⅢ, VIL1 and CLU in the cervical lesions, generally the sensitivity, specifi city and accuracy of the ATⅢ protein were the highest (73.0%, 72.0%, 73.0 %), VIL1 were the next (65.0%, 57.0%, 54.0%), and CLU were the lowest (60.0%, 59.0%, 59.0%). Conclusion The content of plasma proteins ATⅢ, VIL1 and CLU is an important symbol of the process of cervical lesions, and has high predictive value. It is expected to be the early diagnosis of cervical cancer.

     

  • [1] Liu KJ, Liu JW, Li XR, et al. Epidemiology study of risk factors on Uigur and Han cervical cancer in Xinjiang[J]. Xinjiang Yi Ke Da Xue Xue Bao, 2008, 31(10):1335-8. [刘开江, 刘继文, 李晓荣, 等. 新疆维, 汉妇女宫颈癌发病危险因素的流行病学研究[J]. 新 疆医科大学学报, 2008, 31(10):1335-8.]
    [2] Lalai•Suzuke,Peng YH,Zhou K,et al.The analysis of pathogenetic tendency of cervical cancer in various ethnic women in Xinjiang[J].Xinjiang Yi Ke Da Xue Xue Bao,2006,29 (7):569-71. [拉莱•苏祖克, 彭玉华, 周康, 等. 新疆不同民族宫颈癌发病趋 势分析[J]. 新疆医科大学学报, 2006, 29(7):569-71.]
    [3] Zhang GQ, LIU KJ, Lai XJ, et al. Distribution of malignant tumor patients in hospital from 1989 to 2002 in the Affiliated Tumor Hospital of Xinjiang Medical University[J]. Xinjiang Yi Ke Da Xue Xue Bao, 2003, 26(4):393-4. [张国庆,刘开江,赖小军,等.新 医大附属肿瘤医院1989~2002年住院病人恶性肿瘤分布[J].新 疆医科大学学报, 2003, 26(4):393-4.]
    [4] Hao Z,Liu HM,Haimiti•Abudulimu.Expression of HSP90 and HPV16 in cervical cancer in Urghur women[J].Zhong Liu Fang Zhi Yan Jiu,2010,37(1):74-6,80.[郝治,刘红梅,海米提•阿布都力 木.维吾尔族妇女宫颈癌组织中HSP90的表达及其与HPV16的 相关性[J].肿瘤防治研究,2010,37(1):74-6,80.]
    [5] Sölétormos G,Duffy MJ,Hayes DF, et al.Design of tumor biomarker-monitoring trials:a proposal by the European Group on tumor markers[J].Clin Chem,2013,59(1):52-9.
    [6] Zhang L,Tang HQ.Changes and clinical signifi cance of coagulation markers in patients with gastric cancer[J]. Jian Yan Yi Xue Yu Lin Chuang,2012, 9(2):166-7,169. [张琳, 唐沪强. 胃癌患者凝血指 标变化及临床意义[J]. 检验医学与临床, 2012,9(2):166-7,169. ]
    [7] Rickles FR.Mechanisms of cancer-induced thrombosis in cancer[J]. Pathophysiol Haemost Thromb,2006, 35(1-2):103-10.
    [8] Chen N, Xu K. Application and prospect of Heparin antithrombin in cancer therapy[J]. Chongqing Yi Xue,2011,40(20):2062-4.[陈 楠, 徐昆. 肝素抗凝血酶III在肿瘤治疗中的应用与展望[J]. 重庆 医学, 2011,40(20):2062-4.]
    [9] Lee S,Hong SW,Min BH,et al.Essential role of clusterin in pancreas regeneration[J].Dev Dyn,2011,240(3):605-15.
    [10] Ekici S,Ero?lu A,Do?an Ekici AI,et al.Clusterin immunoreactivity as a predictive factor for progression of non-muscle-invasive bladder carcinoma[J]. Urol Int,2011,86(1):31-5.
    [11] Zhang JJ,Yu YL,Du Y.The research progression of clusterin in tumor[J].Neimenggu Yi Xue Za Zhi,2011,43(4):445-8.[张建举, 于跃利,杜颖.Clusterin在肿瘤中的研究进展[J].内蒙古医学杂 志,2011,43(4):445-8.]
    [12] Nakamura E,Iwakawa M,Furuta R,et al. Villin1, a novel diagnostic marker for cervical adenocarcinoma[J].Cancer Biol Ther,2009, 8( 12):1146-53.
    [13] Zavros Y,Eaton KA,Kang W,et al.Chronic gastritis in the hypochlorhydric gastrin-deficient mouse progresses to adenocarcinoma[J].Oncogene,2005,24(14):2354-66.
    [14] Kennedy MT,Jordan RC,Berean KW,et al.Expression pattern of CK7, CK20, CDX-2, and villin in intestinal-type sinonasal adenocarcinoma[J]. J Clin Pathol,2004,57(9):932-7.
计量
  • 文章访问数:  1304
  • HTML全文浏览量:  336
  • PDF下载量:  612
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-05-13
  • 修回日期:  2013-10-16
  • 刊出日期:  2014-06-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭