Abstract: Abstract：Objective To investigate the expression of miR-96 in lung cancer tissues and demonstrate the regulative effects of miR-96 ASO on the invasion and migration of lung cancer cells in vitro. Methods The expression of miR-96 in 116 cases of lung cancer tissues and their adjacent tissues were detected by fl uorogenic quantitative PCR method. The effects of miR-96 ASO on the invasion and migration ability of lung cancer cells were measured by transwell assay and wound healing assay. Invasion-related protein expression was analyzed by Western blot. Results In 116 cases of lung cancer, the expression of miR-96 in 63.80%(74/116) of lung cancer tissues was significantly higher than that in adjacent tissues(P<0.05). miR-96 expression in lung cancer cells in miR-96 ASO transfection group was significantly lower than that in MOCK and NC group(P<0.05). Transwell and wound healing assay results showed that the invasion and migration ability was decreased greatly after transfected with miR-96 ASO, furthermore, down -regulation of miR-96 resulted in obvious inactivation of MMP2 and MMP9(P<0.05). Conclusion miR-96 was up-regulated in human lung cancerous tissue. Reducing the expression of miR-96 can effectively inhibit the invasion and migration of lung cancer cells. miR-96 may become a new target for regulating the invasion and migration ability in lung cancer.